Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing

Kim, Dong-Yoon; Le, Quoc‐Viet; Wu, Yina; Park, Jin-Won; Oh, Yu‐Kyoung

doi:10.3390/pharmaceutics12121233

Cited by 26 publications

(21 citation statements)

References 183 publications

(217 reference statements)

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“…This demonstrates the possibility of using tube particles to transport specialized proteins to nanoparticles that could influence exosome development. In addition, a previous study reported direct engineering of a fluorescent substance into exosomes to confirm trafficking in human cells (Figure 1d(i)) [48,49].…”

Section: Strategies Of Exosome Applications 41 Development Of Exosome Trafficking Systemmentioning

confidence: 93%

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Park

2021

OHBM

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Recently, studies on the mechanism and clinical application of stem cell-derived exosomes have increased. Although the number of patients with hearing loss is increasing, there is no ideal therapy for the recovery of auditory cells of an independent organ in humans. In this review, we proposed the use of stem cell-derived exosomes for treating hearing loss and summarized the exosome research strategy platform for preclinical studies. It is necessary to select a research direction to assess direct or indirect effects on recipients based on the physiological mechanisms of exosomes that deliver useful molecules (called payloads) to recipient cells or tissues. To apply exosomes in the auditory field, researchers should select a model for assessing the toxicity to the auditory cells and analyzing their mechanisms in the recipient tissue. Such in vitro, ex vivo, and in vivo models have been designed and reported in previous studies. The analytical strategies in various models can evaluate the mechanism of exosomes based on exosome surface markers or the payload, thus helping the researchers in finding evidence regarding the efficacy of exosomes. Here, we propose three strategies for exosome application research in the auditory field.

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Section: Strategies Of Exosome Applications 41 Development Of Exosome Trafficking Systemmentioning

confidence: 93%

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Park

2021

OHBM

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“…This was demonstrated by evaluating the cancer-targeting ability of targeted exosomes in tumor transplant animal models and evaluating the anticancer efficacy of romidepsin [110]. CRISPR-Cas9 technology, which is widely used in cell and animal experiments, presents obstacles to its clinical application [113]. The researchers confirmed that the CRISPR-Cas9-sgRNA and Cas9 protein can be packaged into EVs that exist as sgRNA and Cas9 protein complexes of sgRNA: Cas9 ribonucleoprotein [111,112].…”

Section: Ev Engineering To Develop a Targeted Drug Delivery Systemmentioning

confidence: 96%

“…To target destinations, studies reported through engineering modifications of proteins such as CRISPR/CAS9 or aptamers have been introduced [112][113][114]. To verify the efficiency of targeted drug delivery, the histone deacetylase inhibitor lomidepsin was loaded into mAb-exosomes, which showed increased anticancer toxicity in vitro.…”

Section: Ev Engineering To Develop a Targeted Drug Delivery Systemmentioning

confidence: 99%

Engineering of Extracellular Vesicles Based on Payload Changes for Tissue Regeneration

Seo

2021

Tissue Eng Regen Med

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In the field of tissue regeneration and tissue engineering, many years ago, various nano to macroscopic-sized materials have been used to reduce inflammation and restore damaged tissue. Whether it is safe to study the regeneration of all tissues based on the biological mechanisms of an organism composed of cells is still debated, and studies using extracellular vesicles derived from cells have become popular in the past decade. It has been reported that exosomes with a size of 100 nm or less, which plays an important role in cell-cell communication, contain various factors, such as proliferation, anti-inflammatory, and growth factors. In addition, the payload of exosomes varies depending on the parent cell and the recipient cell, and a technology to differentiate the selective payload must treat specific diseases. In this review, we examined the current trends in research using exosomes derived from cells or tissues and analyzed various research reports on factors that can affect tissue regeneration.

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“…Thus, it is essential to improve various aspects of nanovesicles [ 148 ]. For such nanomaterials, techniques and gene delivery approaches continue to be developed, and the key challenge is to balance transfection efficiency, targeting specificity, particle size, biodegradability, and cytotoxicity, as well as their short- and long-term fates in the environment [ 149 ].…”

Section: Future Prospectsmentioning

confidence: 99%

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

Alghuthaymi

Ahmad

Khan

et al. 2021

IJMS

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Rapid developments in the field of plant genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems necessitate more detailed consideration of the delivery of the CRISPR system into plants. Successful and safe editing of plant genomes is partly based on efficient delivery of the CRISPR system. Along with the use of plasmids and viral vectors as cargo material for genome editing, non-viral vectors have also been considered for delivery purposes. These non-viral vectors can be made of a variety of materials, including inorganic nanoparticles, carbon nanotubes, liposomes, and protein- and peptide-based nanoparticles, as well as nanoscale polymeric materials. They have a decreased immune response, an advantage over viral vectors, and offer additional flexibility in their design, allowing them to be functionalized and targeted to specific sites in a biological system with low cytotoxicity. This review is dedicated to describing the delivery methods of CRISPR system into plants with emphasis on the use of non-viral vectors.

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Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing

Cited by 26 publications

References 183 publications

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Engineering of Extracellular Vesicles Based on Payload Changes for Tissue Regeneration

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

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