Nanotoxicity of TiO2 nanoparticles to erythrocyte in vitro

Li, Shi-Qiang; Zhu, Rongrong; Hong, Zhu; Meng, Xue; Sun, Xiaoyu; Yao, Side; Wang, Shilong

doi:10.1016/j.fct.2008.09.012

Cited by 214 publications

(160 citation statements)

References 33 publications

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“…Indeed, it was recently reported that MTX incorporated in liposomal formulations displayed higher hemoreactivity than its free solution [47]. The agglutination induced by the NPs at the highest concentration tested may be caused by increased binding of the NPs to the erythrocyte membrane, which might deformed cells and hence decrease the repulsion among them [48].…”

Section: Discussionmentioning

confidence: 99%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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Nanoparticles with pH-sensitive behavior may enhance the success of chemotherapy in many cancers by efficient intracellular drug delivery. Here, we investigated the effect of a bioactive surfactant with pH-sensitive properties on the antitumor activity and intracellular behavior of methotrexate-loaded chitosan nanoparticles (MTX-CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included the surfactant derived from N α ,N ε -dioctanoyl lysine with an inorganic lithium counterion. The pH-sensitive behavior of NPs allowed accelerated release of MTX in an acidic medium, as well as membrane-lytic pH-dependent activity, which facilitated the cytosolic delivery of endocytosed materials. Moreover, our results clearly proved that MTX-CS-NPs were more active against the tumor HeLa and MCF-7 cell lines than the free drug. The feasibilty of using NPs to target acidic tumor extracellular pH was also shown, as cytotoxicity against cancer cells was greater in a mildly acidic environment. Finally, the combined physicochemical and pH-sensitive properties of NPs generally allowed the entrapped drug to induce greater cell cycle arrest and apoptotic effects. Therefore, our overall results suggest that pH-sensitive MTX-CS-NPs could be potentially useful as a carrier system for tumor and intracellular drug delivery in cancer therapy.

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Section: Discussionmentioning

confidence: 99%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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“…With frequent exposure to dispersed nanoparticles from the composite products or workplaces, there is an increased chance for nanoparticles or nano composites to enter human body and to relocate in metabolism-active organs (Song et al 2009). Thus, studying the toxicity of nanomaterials is of importance to provide the guidance to occupational health and safety (Li et al 2008;Lanone et al 2009;Turkez et al 2013a). The discussion about safety concerns associated with small particles is ongoing for many decades and, to a large extent, is related to the potential risks following inhalative, oral, parenteral or dermal exposure ).…”

Section: Introductionmentioning

confidence: 99%

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Türkez

Sönmez

Turkez

et al. 2014

Braz. arch. biol. technol.

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“…13 In vitro, nanoparticles can penetrate the cell membrane by diffusion or adhesion, 36 and the size of these particles could be an important factor. The reaction between the particles and the cell membrane results in generation of ROS, and the oxidative stress generated might cause breakdown of the membrane lipids, 37,38 which might lead to fusion of the cell membrane and result in multinucleation. Therefore, increased cellular ROS after exposure to silica could be one of the reasons for cell fusion and subsequent cell multinucleation.…”

Section: Apoptosis Of L-02 Cellsmentioning

confidence: 99%

Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line

Wang¹,

Li²,

Liu³

et al. 2013

IJN

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Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.

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Nanotoxicity of TiO2 nanoparticles to erythrocyte in vitro

Cited by 214 publications

References 33 publications

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line

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