Nanothymoquinone, a novel hepatotargeted delivery system for treating CCl4 mediated hepatotoxicity in rats

Verma, Shashi Kant; Rastogi, Shweta; Javed, Kalim; Akhtar, Mohd; Arora, Indu; Samim, Mohd

doi:10.1039/c3tb20379d

Cited by 10 publications

(12 citation statements)

References 39 publications

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“…In the FTIR study, we showed that BSO is enriched with various components and functional groups such as C=O, C-O, C-H, CH 3 , and CH 2 chemical constituents. Other researchers believed that the wide application of black seed oil in medicine was ascribed to the existence of TQ constituents [58–60]. It is well reported that Nigella sativa has been broadly used since very ancient times as a food additive or natural medicine for a large variety of diseases, but the mechanism of its action is still being studied [61].…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil

Mohammed

Amin

Aziz

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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This study was aimed to investigate the structure of bioactive components of black seed oil (BSO) and their antimicrobial and cytotoxic effects. Initially, the structural examination was conducted using various spectroscopic techniques, such as FTIR, TLC, and UV-visible spectroscopy, which are important in determining substituents, functional groups, and the presence of conjugated double bonds in BSO. From the FTIR spectra, a variety of sharp, strong, and weak peaks were specified relating to the main components of thymoquinone (TQ), dithymoquinone, thymohydroquinone, and thymol in BSO. The results of UV-visible spectroscopy confirmed the presence of thymoquinone as a major compound, and conjugated double bonds were also found. In addition, qualitative TLC analysis was used to identify thymoquinone from the methanol-extracted layer in BSO, by calculating the retention factor (Rf) value. Furthermore, antimicrobial activity of BSO was studied against various types of bacteria. Strong bacterial inhibitory effects were observed, especially against Bacillus subtilis, with an average inhibition zone of 15.74 mm. Moreover, through the use of the MTT assay in vitro, it was shown that BSO does not exhibit any cytotoxicity towards human peripheral blood mononuclear cells (PBMCs). It was also found from the structural characterization of BSO that the existence of TQ is responsible for potential antibacterial activity without any cytotoxic effects. The main observation of this work is that BSO has antimicrobial activity even against methicillin-resistant Staphylococcus aureus (MRSA).

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Section: Resultsmentioning

confidence: 99%

Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil

Mohammed

Amin

Aziz

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Drug was encapsulated into the core of the nanopolymer . Briefly, 5 mg riluzole was dissolved in 1 mL DMSO, and it was slowly added into the above dialyzed solution with vortexing.…”

Section: Methodsmentioning

confidence: 99%

“…Drug was encapsulated into the core of the nanopolymer. 23 Briefly, 5 mg riluzole was dissolved in 1 mL DMSO, and it was slowly added into the above dialyzed solution with vortexing. It was then dialyzed for 8 h, and distilled water was changed continuously every 2 h to remove DMSO.…”

Section: ■ Introductionmentioning

confidence: 99%

Enhancement in the Neuroprotective Power of Riluzole Against Cerebral Ischemia Using a Brain Targeted Drug Delivery Vehicle

Verma

Arora

Javed

et al. 2016

ACS Appl. Mater. Interfaces

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Riluzole is the only available drug for motor neuron diseases quite well-known for its neuroprotective activity. But its poor aqueous solubility, short half-life with some side-effects at higher concentration poses a limitation to its use as a therapeutic agent. The present study was performed to investigate the therapeutic potential of nanoriluzole (NR), i.e., riluzole encapsulated in nanoparticles against cerebral ischemia (stroke) at three different concentrations [10 (NRL), 20 (NRM), and 40 (NRH) μg/kg body weight intraperitoneally (i.p.)]. Chitosan conjugated NIPAAM (N-isopropylacrylamide) nanoparticles coated with tween80 were synthesized through free radical polymerization. The particles were characterized with Transmission Electron Microscopy, Dynamic Light Scattering, and Fourier Transform Infrared spectroscopy and were found to have size of ∼50 nm. Cerebral ischemia was induced by Middle Cerebral Artery Occlusion (MCAO) model for 1 h and NR was given intraperitoneally after 1 h of MCAO. Animals were dissected after a reperfusion period of 24 h for evaluation of various parameters. Triphenyl tetrazolium chloride staining shows substantial reduction in infarct size in all three treated groups. It was also supported by histopathological results, biochemical parameters, and behavioral studies. Immunological parameters like NOS-2, NF-kB, and COX-2 also show profound reduction in expression in NR treated groups. Thus, the present work clearly demonstrated that the nanoparticle was good enough to carry large amount of drug across the Blood Brain Barrier which results in significant neuroprotection even at a very low concentration. It also substantially lowered the required concentration by overcoming the poor aqueous solubility; hence hardly leaving any scope for side-effects.

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“…One recent study has reported the synthesis of PAG coated NIPAAM nanoparticles that are encapsulated with TQ for direct hepato-targeting. NIPAAM is a thermosensitive nanopolymer which is widely used as a successful drug delivery system against various diseases and PAG is a galactosylated moiety that targets the liver by interacting with asialoglycoprotein receptor (ASGP-R) present on the surface of hepatocytes and delivers the drug directly to the liver (Verma et al 2013 ). The toxicity of the nanocarrier (NIPAAM) at this concentration is almost negligible and due to the size of the nanoparticle being smaller than the already reported nanothymoquinone.…”

Section: Chemistry Of the Alkaloids And Tq In Nigella Sativmentioning

confidence: 99%

Chemical composition of Nigella sativa Linn: Part 2 Recent advances

Khan

Afzal

2016

Inflammopharmacol

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The black cumin or Nigella sativa L. seeds have many acclaimed medicinal properties such as bronchodilatory, hypotensive, antibacterial, antifungal, analgesic, anti-inflammatory and immunopotentiating. This review article is an update on the previous article published on Nigella sativa L. in this journal in 1999. It covers the medicinal properties and chemical syntheses of the alkaloids isolated from the seeds of the herb.

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Nanothymoquinone, a novel hepatotargeted delivery system for treating CCl4 mediated hepatotoxicity in rats

Cited by 10 publications

References 39 publications

Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil

Structural Characterization, Antimicrobial Activity, and In Vitro Cytotoxicity Effect of Black Seed Oil

Enhancement in the Neuroprotective Power of Riluzole Against Cerebral Ischemia Using a Brain Targeted Drug Delivery Vehicle

Chemical composition of Nigella sativa Linn: Part 2 Recent advances

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