Nanostructured lipid systems modified with waste material of propolis for wound healing: Design, in vitro and in vivo evaluation

Rosseto, Hélen Cássia; Toledo, Lucas de Alcântara Sica de; Francisco, Lizziane Maria Belloto de; Esposito, Elisabetta; Lim, Yunsook; Valacchi, Giuseppe; Cortesi, Rita; Bruschi, Marcos Luciano

doi:10.1016/j.colsurfb.2017.07.029

Cited by 61 publications

(27 citation statements)

References 80 publications

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“…The rapid evaporation of ethanol should be also considered. Moreover, the polarity and chemical composition of PE enable good retention and permeation ( de Toledo et al, 2015 , 2016 ; Rosseto et al, 2017 ). Therefore, PE may constitute a good final formulation to be administered to the nail.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, there is concern about the use of over-the-counter and natural remedies for onychomycosis ( Halteh et al, 2016 ), considering the need for standardization and quality control, among other requirements, to produce reliable compounds. These concerns have been allayed for propolis, because the products containing propolis as the active principle are registered, regulated, and approved for use in several pharmaceutical dosage forms in South America (e.g., Brazil and Argentina), the United States, Europe, and Asia (e.g., Japan) ( de Toledo et al, 2015 , 2016 ; Rosseto et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

et al. 2018

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Onychomycosis is a chronic fungal infection of nails, commonly caused by dermatophyte fungi, primarily species of Trichophyton. Because of the limited drug arsenal available to treat general fungal infections and the frequent failure of onychomycosis treatment, the search for new therapeutic sources is essential, and topical treatment with natural products for onychomycosis has been encouraged. Propolis, an adhesive resinous compound produced by honeybees (Apis mellifera), has shown multiple biological properties including significant antifungal and anti-biofilm activities in vitro. In spite of promising in vitro results, in vivo results have not been reported so far. This study assessed an ethanol propolis extract (PE) as a topical therapeutic option for onychomycosis, including its characterization in vitro and its applicability as a treatment for onychomycosis (from bench to clinic). The in vitro evaluation included analysis of the cytotoxicity and the antifungal activity against the planktonic cells and biofilm formed by Trichophyton spp. We also evaluated the capacity of PE to penetrate human nails. Patients with onychomycosis received topical PE treatments, with a 6-month follow-up period. The results of the in vitro assays showed that PE was non-toxic to the cell lines tested, and efficient against both the planktonic cells and the biofilm formed by Trichophyton spp. The results also showed that PE is able to penetrate the human nail. The results for PE applied topically to treat onychomycosis were promising, with complete mycological and clinical cure of onychomycosis in 56.25% of the patients. PE is an inexpensive commercially available option, easy to obtain and monitor. Our results indicated that PE is a promising natural compound for onychomycosis treatment, due to its ability to penetrate the nail without cytotoxicity, and its good antifungal performance against species such as Trichophyton spp. that are resistant to conventional antifungals, both in vitro and in patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

et al. 2018

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“…That is why propolis is commonly used as a liquid ethanolic extract [ 7 ]. Ethanolic extracts are convenient for certain purposes of external use [ 8 ]. However, strong and unpleasant taste as well as irritating solvent limits peroral administration capacity of this extract, does not allow to use it for ophthalmic and pediatric cases and decreases the applicability in pharmaceutical and cosmetics industry.…”

Section: Introductionmentioning

confidence: 99%

Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating properties

Kubilienė

Jekabsone

Žilius

et al. 2018

BMC Complement Altern Med

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BackgroundPropolis is multicomponent substance collected by honeybees from various plants. It is known for numerous biological effects and is commonly used as ethanolic extract because most of active substances of propolis are ethanol-soluble. However, water-based propolis extracts could be applied more safely, as this solvent is more biocompatible. On the other hand, water extracts has significantly smaller range and quantity of active compounds. The extraction power of water could be enhanced by adding co-solvent which increases both solubility and penetration of propolis compounds. However, variation of solvents results in different composition of active substances that might have distinct effects. The majority of biological effects of propolis are attributed to the antioxidant properties of its active compounds. Antioxidant effect might be a result of either direct scavenging of ROS or modulation of ROS producing organelle activity. Therefore, the aim of this study was to investigate and compare chemical composition, antioxidant properties and effects on mitochondrial respiration of aqueous (AqEP), polyethylene glycol-aqueous (Pg-AqEP) and ethanolic (EEP) propolis extracts.MethodsChemical composition of propolis extracts was determined using HPLC and Folin-Ciocalteu method. Ability to neutralize H2O2 and intracellular ROS concentration in C6 glioma cells were determined fluorometrically by using 10-acetyl-3,7-dihydroxyphenoxazine and 2′,7′-dichlorofluorescein diacetate, respectively. Mitochondrial superoxide generation was assessed under fluorescent microscope by using MitoSOX Red. Oxygen uptake rates of mitochondria were recorded by high-resolution respirometer Oxygraph-2 k.ResultsOur data revealed that phenolic acids and aldehydes make up 40–42% of all extracted and identified compounds in AqEP and Pg-AqEP and only 16% in EEP. All preparations revealed similar antioxidant activity in cell culture medium but Pg-AqEP and EEP demonstrated better mitochondrial superoxide and total intracellular ROS decreasing properties. At higher concentrations, AqEP and EEP inhibited mitochondrial respiration, but Pg-AqEP had concentration-dependent mitochondria-uncoupling effect.ConclusionsAqueous and non-aqueous propolis extracts differ by composition, but all of them possess antioxidant properties and neutralize H2O2 in solution at similar efficiency. However, both Pg-AqEP and EEP were more effective in decreasing intracellular and intramitochondrial ROS compared to AqEP. At higher concentrations, these preparations affect mitochondrial functions and change energy production in C6 cells.

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“…Noteworthy is the use of honey as a wound-healing agent since it promotes a multifaceted and safe healing activity for all skin wounds [13]. Moreover, either ethanolic [19] or aqueous [20] extract of propolis accelerates wound healing and have synergistic action with honey [21,22].…”

Section: Resultsmentioning

confidence: 99%

Apitherapy of Septic Metacarpal and Metatarsal Wounds (An Experimental Study on Donkeys)

Hamouda¹,

Kuraa²,

El-Samieh³

et al. 2019

CMR

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Non-healed wounds are a significant problem in the healthcare system of the distal portions of the limbs. Nine types of honey were tested for wound healing judged by clinical gross healing parameters, rate of wound contraction and sings of epithelization histopathologically. Rounded full thickness skin wounds (1.5cm diameter) were performed at the distal portion of limbs. The wounds were infected by standard dose of MRSA (methicillin resistance Staph. aureus) for 72 hours. The tested types of honey were tested for MIC & MBC against clinical MRSA strain. Different types of honey were applied unprocessed as daily topical wound dressing for the infected wounds. Propolis with black seed honey followed by Hadramout sidr honey, then Egyptian sidr honey proved to have the most antimicrobial activity. Clinical healing appearance revealed that propolis with black seed honey, Egyptian sidr honey and Hadramout sidr honey showed the most preferable clinical parameters (gross appearance and rate of wound contraction), and histopathologically achieved the best healing epithelization 28 days after treatment. The study concluded that among different nine honey types retailed in Egyptian markets, all tested honey types had wound healing activities but with different potencies. Propolis with black seed, Egyptian sidr honey and Hadramout honey were the most potent with highly significance differences (P < 0.01), since achieving the most wound contraction rates, rapid epithelization post 28 days rather than the least MICs & MBCs values. While the marjoram and clover honey were the least effective.

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Nanostructured lipid systems modified with waste material of propolis for wound healing: Design, in vitro and in vivo evaluation

Cited by 61 publications

References 80 publications

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating properties

Apitherapy of Septic Metacarpal and Metatarsal Wounds (An Experimental Study on Donkeys)

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