Nanostructured Layer-by-Layer Polyelectrolyte Containers to Switch Biofilm Fluorescence

Abstract

The development of stimuli-responsive nanocontainers is an issue of utmost importance for many applications such as targeted drug delivery, regulation of the cell and tissue behavior, making bacteria have useful functions and here converting light. The present work shows a new contribution to the design of polyelectrolyte (PE) containers based on surface modified mesoporous titania particles with deposited Ag nanoparticles to achieve chemical light upconversion via biofilms. The PE shell allows slowing down the kinetics of a release of loaded l-arabinose and switching the bacteria luminescence in a certain time. The hybrid TiO2/Ag/PE containers activated at 980 nm (IR) illumination demonstrate 10 times faster release of l-arabinose as opposed to non-activated containers. Fast IR-released l-arabinose switch bacteria fluorescence which we monitor at 510 nm. The approach described herein can be used in many applications where the target and delayed switching and light upconversion are required.

Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

“…Thus, CuO NPs are toxic enough for bacteria and have shown significant effects against biofilms. TiO2 NPs under irradiation can generate electron-hole pairs with photoexcitation, initiate cascade oxidation-reduction reactions on the surface of TiO2, and, consequently, produce ROS for further reactions [50,51]. Thus, TiO2 NPs inhibit bacterial growth by lipid peroxidation in the membranes, DNA damage, nucleotide and amino acid oxidation, or destruction of protein-catalytic sites by photocatalysis [52].…”

Nanoparticles in Polyelectrolyte Multilayer Layer-by-Layer (LbL) Films and Capsules—Key Enabling Components of Hybrid Coatings

“…Photocatalytic reduction [107] of noble metal particles on titania core [95] leads to dual-wavelength responsiveness: in the UV to near-IR regions of electromagnetic spectrum. It is possible to regulate photocatalytically (auto-catalytic) waves of enzymatic reactions [108], switch biofilm fluorescence [109], build a platform for a chemical logic device [110], and perform sustainable diagnostics [111][112][113]. The trend in the field of encapsulated systems is to combine different functions in one capsule matrix.…”

Shannon entropy associated with electrochemically generated ion concentration gradients

“…The LbL approach provides a versatile instrument for multilayer formation with the incorporation of variously charged compounds [8,9]. Polyelectrolyte multilayers are widely used in the design of biomaterial surface coatings [10,11], membranes for separation, as well as cargoes for drug encapsulation and delivery [12].…”