Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection

Kaushik, Ajeet; Solanki, Pratima R.; Kaneto, Keiichi; Kim, C. G.; Ahmad, Sharif; Malhotra, Bansi D.

doi:10.1002/elan.200900468

Cited by 49 publications

(47 citation statements)

References 37 publications

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“…The surface concentration of the redox species (G) for NanoZrO 2 /ITO and ssDNA/NanoZrO 2 /ITO bioelectrode has been found to be as 5.52 10 À9 mol/cm 2 and 6.01 10 À9 mol/cm 2 , respectively, using the Lavirons equation [20]. These results reveal that the more redox species are present onto ssDNA/NanoZrO 2 /ITO bioelectrode as compared to that of NanoZrO 2 /ITO electrode resulting in stronger interaction between the oxygen molecules of zirconium species and phosphate group of DNA backbone.…”

Section: à6mentioning

confidence: 99%

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

et al. 2011

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O1 gene based 24-mer single stranded deoxyribonucleic acid probe (ssDNA) has been immobilized onto sol-gel derived nanostructured zirconium oxide (NanoZrO 2 ) film fabricated onto indium-tin-oxide (ITO) coated glass plate to detect Vibrio cholerae. The X-ray diffraction and Atomic Force Microscopy techniques have been used to characterize the nanostructured ZrO 2 (particle size of~30-40 nm) and the ssDNA/ZrO 2 bioelectrode. The hybridization of ssDNA/ZrO 2 bioelectrode with the complementary and genomic DNA has been investigated using differential pulse voltammetry. The results of electrochemical studies suggest that electro-active and cationic NanoZrO 2 provides an effective surface to bind with the phosphate group of DNA resulting in enhanced electron transport. The ssDNA/NanoZrO 2 bioelectrode shows a detection range from 1 10 À8 to 10 nM of complementary DNA of V. cholerae within 60 s of hybridization time at 25 8C using methylene blue as an electroactive indicator. This O1 gene based metal oxide (ZrO 2 ) sensor exhibits sensitivity for ssDNA/NanoZrO 2 /ITO bioelectrode as 0.48 mA/nM cm À2 for complementary DNA and 2.34 mA/nM cm À2 for genomic DNA with regression coefficients (R) of 0.991 and 0.995, respectively. This DNA bioelectrode is stable for about 15 weeks when stored at 4 8C.

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Section: à6mentioning

confidence: 99%

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

et al. 2011

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“…The EIS method is one of the best non-invasive analytical tool and is being explored for monitoring biological phenomena such as protein-protein interaction (Bogomolova et al 2009; Chang and Park 2010; Franks et al 2005), bio-sensing (Kaushik et al 2010), nano-toxicity (Shah et al 2014) and tumour drug interaction (Luongo et al 2013). An EIS based detection method, using 10 mM [Fe(CN) 6 ] 3−/4− as the redox probe in 50 mM PBS with 100 mM NaCl (pH 7.4)) at a potential of 0.30 V was developed to detect β-A.…”

Section: Advancements Of Electrochemical β-A Bio-sensingmentioning

confidence: 99%

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Kaushik

Jayant

Tiwari

et al. 2016

Biosensors and Bioelectronics

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Beta-amyloid (β-A) peptides are potential biomarkers to monitor Alzheimer’s diseases (AD) for diagnostic purposes. Increased β-A level is neurotoxic and induces oxidative stress in brain resulting in neurodegeneration and causes dementia. As of now, no sensitive and inexpensive method is available for β-A detection under physiological and pathological conditions. Although, available methods such as neuroimaging, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) detect β-A, but they are not yet extended at point-of-care (POC) due to sophisticated equipments, need of high expertise, complicated operations, and challenge of low detection limit. Recently, β-A antibody based electrochemical immuno-sensing approach has been explored to detect β-A at pM levels within 30–40 minutes compared to 6–8 hours of ELISA test. The introduction of nano-enabling electrochemical sensing technology could enable rapid detection of β-A at POC and may facilitate fast personalized health care delivery. This review explores recent advancements in nano-enabling electrochemical β-A sensing technologies towards POC application to AD management. These analytical tools can serve as an analytical tool for AD management program to obtain bio-informatics needed to optimize therapeutics for neurodegenerative diseases diagnosis management

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“…Th is sensor shows linearity up to 50 mM, sensitivity of 43. [36]. In this system, the charge-transfer resistance decreases with increasing cholesterol concentration ( Figure 5).…”

Section: Cholesterol Oxidasementioning

confidence: 99%

Nanostructured metal oxide-based biosensors

et al. 2011

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A mong the various types of nanomaterials that have been developed, nanostructured metal oxides (NMOs) have recently aroused much interest as immobilizing matrices for biosensor development ( Figure 1) [1][2][3][4][5][6][7][8][9]. Nanostructured oxides of metals such as zinc, iron, cerium, tin, zirconium, titanium, metal and magnesium have been found to exhibit interesting nanomorphological, functional biocompatible, non-toxic and catalytic properties. Th ese materials also exhibit enhanced electron-transfer kinetics and strong adsorption capability, providing suitable microenvironments for the immobilization of biomolecules and resulting in enhanced electron transfer and improved biosensing characteristics. Various morphologies of NMOs have been obtained using a variety of methods, including soft templating for the preparation of nanorods and nanofi bers [10], sol-gel methods for the production of three-dimensional (3D) ordered rough nanostructures [6,11], radiofrequency sputtering for rough nanostructures [4] and hydrothermal deposition for nanoparticles with controlled shape [12]. All of these NMOs have been reported to have potential applications in biosensors. Recently, the optical, electrical and magnetic properties of NMOs have been reported to be enhanced through the incorporation of nanoparticles of conducting or semiconducting materials such as carbon nanotubes, graphene, gold and silver, as well as quantum dots of various semiconductors, with advantages for improved biosensor characteristics [13][14][15][16]. It is expected that the judicious application of an NMO may lead to the fabrication of novel biosensing devices with enhanced signal amplifi cation and coding strategies for bioaffi nity assays and effi cient electrical communication with redox biomolecules/ enzymes that may address future diagnostic needs.Th e unique properties of NMOs off er excellent prospects for interfacing biological recognition events with electronic signal transduction and for designing a new generation of bioelectronics devices that may exhibit novel functions. Th e controlled preparation of an NMO is considered to play a signifi cant role in the development of biosensors. Eff orts are being made to explore the prospects and future challenges of NMOs for the development of biosensing devices and the evolution of new strategies for bioaffi nity assays and effi cient electrical communication. In this review, we focus on developments over the past fi ve years in NMO-based biosensors for clinical and non-clinical applications (Figure 2). Fundamentals of nanostructured metal oxides for biosensingAmong the various immobilizing matrices that have been developed, NMOs have exceptional optical and electrical properties due to electron and phonon confi nement, high surface-to-volume ratios, modifi ed surface work function, high surface reaction activity, high catalytic effi ciency and strong adsorption ability. For these reasons, NMOs have been utilized to increase the loading of biomolecules per Nanostructured metal oxide-based b...

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Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection

Cited by 49 publications

References 37 publications

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Nanostructured metal oxide-based biosensors

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