Nanostructure and molecular accessibility of gel-type resins for supported bio-catalysis

Catalytic activity of bovine lactoperoxidase supported on macroporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

“…These parameters are reported in Table 3, together with the isotropic 14 N splitting constant a N , which is related to the polarity of the medium surrounding the spin-probe. s and a N referring to the site b are comparable with the values observed in bulk water (13 ps and 15.85 G, respectively, [6]). On the other hand, the rotational diffusion of the spin-probe located in the site a appears to be significantly hindered (s is noticeably greater as compared with the value observed in bulk water).…”

“…In previous work [6] we started to evaluate the possibility of obtaining amphiphilic hydroxylic resins potentially suitable both to effectively support in their interior a given enzyme and to regulate the molar fraction of water in contact with an immiscible organic solvent inside the polymer framework of a given resin particle. In this paper we report on a protocol suitable to both the anchoring of lactoperoxidase (LPO), as a model enzyme, onto a macroporous hydroxylic resin and to stabilise the enzyme without producing a marked damping of catalytic activity.…”