Nanoscale‐Controlled Enzymatic Degradation of Poly(<scp>L</scp>‐lactic acid) Films Using Dip‐Pen Nanolithography

Li, Hai; He, Qiyuan; Wang, Xiaohong; Lü, Gang; Liusman, Cipto; Li, Bing; Boey, Freddy Yin Chiang; Venkatraman, Subbu S.; Zhang, Hua

doi:10.1002/smll.201001977

Cited by 25 publications

(23 citation statements)

References 52 publications

(48 reference statements)

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“…The PLLA thin film was obtained by spin‐coating PLLA dichloromethane solution (3.0 wt%) on the cleaned glass substrate at 3000 rpm 17. The PDMS film was fabricated by pouring a mixture of Sylgard 184 elastomer and curing agent (w:w = 10:1) over a clean petri dish and heating at 70 °C for 12 h after degassing 18.…”

Section: Methodsmentioning

confidence: 99%

Graphene Oxide Scrolls on Hydrophobic Substrates Fabricated by Molecular Combing and Their Application in Gas Sensing

et al. 2012

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Well-aligned graphene oxide (GO) scrolls are prepared through the controlled folding/scrolling of single-layer GO sheets using molecular combing on hydrophobic substrates, such as aged gold substrate, polydimethylsiloxane film, poly(L-lactic acid) film, and octadecyltrimethoxysilane-modified silicon dioxide. As a proof of concept, the gas sensor fabricated with a single reduced GO scroll is used to detect NO(2) gas with a concentration as low as 0.4 ppm.

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Section: Methodsmentioning

confidence: 99%

Graphene Oxide Scrolls on Hydrophobic Substrates Fabricated by Molecular Combing and Their Application in Gas Sensing

et al. 2012

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“…For instance, agarose27 and phospholipids17 have been used as carriers for biomolecular ink. Poly(ethylene glycol) (PEG) has also been described as a matrix to facilitate DPN writing of different inorganic materials, such as gold and Fe 3 O 4 nanoparticles, fullerene C 60 , or proteins on various hydrophobic surfaces 28–30. Direct writing of DNA molecules via DPN can also be accomplished in a diffusive31 or liquid writing regime 27.…”

Section: Solid Substrates For Dpn Writing Of Dna Strandsmentioning

confidence: 99%

Biochips for Cell Biology by Combined Dip‐Pen Nanolithography and DNA‐Directed Protein Immobilization

Arrabito

Reisewitz

Dehmelt

et al. 2013

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A general methodology for patterning of multiple protein ligands with lateral dimensions below those of single cells is described. It employs dip pen nanolithography (DPN) patterning of DNA oligonucleotides which are then used as capture strands for DNA-directed immobilization (DDI) of oligonucleotide-tagged proteins. This study reports the development and optimization of PEG-based liquid ink, used as carrier for the immobilization of alkylamino-labeled DNA oligomers on chemically activated glass surfaces. The resulting DNA arrays have typical spot sizes of 4-5 μm with a pitch of 12 μm micrometer. It is demonstrated that the arrays can be further functionalized with covalent DNA-streptavidin (DNA-STV) conjugates bearing ligands recognized by cells. To this end, biotinylated epidermal growth factor (EGF) is coupled to the DNA-STV conjugates, the resulting constructs are hybridized with the DNA arrays and the resulting surfaces used for the culturing of MCF-7 (human breast adenocarcinoma) cells. Owing to the lateral diffusion of transmembrane proteins in the cell's plasma membrane, specific recruitment and concentration of EGF receptor can be induced specifically at the sites where the ligands are bound on the solid substrate. This is a clear demonstration that this method is suitable for precise functional manipulations of subcellular areas within living cells.

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“…Enzyme nanostructures transferred by PEG could effectively degrade poly( L -lactic acid) fi lms sitespecifi cally. [ 34 ] Another key advantage of the polymer carrier system is that it can modulate the transport rate of the polymer/functional material mixture because the transport characteristics of the polymer carrier dominate the system. This is especially important for achieving a similar feature size using different inks in the multiplex patterning of biological materials.…”

Section: Polymeric Carriers For Functional Materialsmentioning

confidence: 99%

Polymer Nanostructures Made by Scanning Probe Lithography: Recent Progress in Material Applications

Xie

Zhou

Tao

et al. 2012

Macromol. Rapid Commun.

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Scanning probe lithography (SPL) is a series of techniques that utilizes a scanning probe or an array of probes for surface patterning. Recent developments of new material systems and patterning approaches have made SPL a promising, low‐cost, bench‐top, and versatile tool for fabricating various polymer nanostructures, with extraordinary importance in physical sciences, life sciences and nanotechnology. This feature article highlights the recent progress in four material applications: polymer resists, polymeric carriers for patterning functional materials, electronically active polymers and polymer brushes for tailoring surface morphology and functionality. An overview of future possibilities, with regard to challenges and opportunities in this field, is given at the end of the paper.

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Nanoscale‐Controlled Enzymatic Degradation of Poly(L‐lactic acid) Films Using Dip‐Pen Nanolithography

Cited by 25 publications

References 52 publications

Graphene Oxide Scrolls on Hydrophobic Substrates Fabricated by Molecular Combing and Their Application in Gas Sensing

Graphene Oxide Scrolls on Hydrophobic Substrates Fabricated by Molecular Combing and Their Application in Gas Sensing

Biochips for Cell Biology by Combined Dip‐Pen Nanolithography and DNA‐Directed Protein Immobilization

Polymer Nanostructures Made by Scanning Probe Lithography: Recent Progress in Material Applications

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