Nanos3 not nanos1 and nanos2 is a germ cell marker gene in large yellow croaker during embryogenesis

Han, Kunhuang; Chen, Shihai; Cai, Mingyi; Jiang, Yonghua; Zhang, Ziping; Wang, Yilei

doi:10.1016/j.cbpb.2018.01.002

Cited by 16 publications

(7 citation statements)

References 54 publications

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“…We have used two nanos 3'UTR, the nanos1 3'UTR from zebra sh, commonly used for PGC labeling of various sh species, and nanos3 3'UTR from P. mangurus. Only nanos3 homolog was found in the egg transcriptome of P. mangurus (data not published), corroborating with the results obtained using Larimichthys crocea, that only nanos3 transcripts signals were detected during embryogenesis, suggesting nanos3, not nanos1 and nanos2 is a germ cell marker gene in this species (Han et al, 2018).…”

Section: Discussionsupporting

confidence: 87%

Primordial germ cells identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.

Monzani

Shiguemoto

Coelho

et al. 2022

Preprint

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Primordial germ cells (PGCs) are responsible for the generation of all germ cells, and therefore, they are essential targets to be used as a tool for the production of germline chimera. The labeling and route of PGCs were evaluated during the initial embryonic development of Pseudopimelodus mangurus, using whole-mount in situ hybridization (WISH) and mRNA microinjection in zygotes. The WISH method was synthesized a specific RNA antisense probe using part of the coding region from P. mangurus nanos3 cDNA. The RNA microinjection was performed using the GFP gene reporter regulated by translation regulatory P. mangurus bucky ball and nanos3 3’UTR sequences, which are germline-specific markers used to describe in vivo migration of PGCs. The nanos3 and bucky ball gene expressions were evaluated in tissues for male and female adults and initial development phases and larvae from first to seventh days post-hatching. The results from the WISH technique appointed to the origin of PGCs in P. mangurus from the aggregations of nanos3 mRNA in the cleavage grooves and the signals obtained of nanos3 probes corresponded topographically to the migratory patterns of the PGC reported for other fish species. The microinjection of GFP-nanos3 3'UTR mRNA in the zygotes of P. mangurus was not successful for PGC labeling, but it was possible to identify three embryos injected, presenting 3 to 5 evidence of possible PGC labeled in the hatching phase. The nanos3 and bucky ball gene expression was reported in the female gonads and from fertilized eggs until the blastula phase. These results provide information about the PGC migration of P. mangurus and the possible use of PGCs for the future generation of germline chimera to be applied in the conservation efforts of Neotropical Siluriformes species, contributing to the establishment of genetic banks, manipulation of organisms, and assisting in biotechnologies such as the transplantation of germ cells in fish.

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Section: Discussionsupporting

confidence: 87%

Primordial germ cells identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.

Monzani

Shiguemoto

Coelho

et al. 2022

Preprint

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“…The Nanos gene encodes an RNA-binding protein and was first identified as a determinant of the anterior-posterior axis in Drosophila melanogaster [1]. Several nanos homologous genes, including Nanos1a , Nanos1b , Nanos2 , and Nanos3 , have been characterized in diverse species since then [2,3,4,5,6]. Accordingly, their evolutionarily conserved roles in germ cell development have been revealed in both invertebrates [7] and vertebrates [8].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Zhang

Han

Wang

et al. 2019

IJMS

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Sea urchin (Mesocentrotus nudus) is an economically important mariculture species in China and the gonads are the solely edible parts to human. The molecular mechanisms of gonad development have attracted increasing attention in recent years. Although the nanos2 gene has been identified as a germ cell marker in several invertebrates, little is known about nanos2 in adult sea urchins. Hereinto, we report the characterization of Mnnano2, an M. nudus nanos2 homology gene. Mnnanos2 is a maternal factor and can be detected continuously during embryogenesis and early ontogeny. Real-time quantitative PCR (RT-qPCR) and section in situ hybridization (ISH) analysis revealed a dynamic and sexually dimorphic expression pattern of Mnnano2 in the gonads. Its expression reached the maximal level at Stage 2 along with the gonad development in both ovary and testis. In the ovary, Mnnanos2 is specifically expressed in germ cells. In contrast, Mnnanos2 is expressed in both nutritive phagocytes (NP) cells and male germ cells in testis. Moreover, knocking down of Mnnanos2 by means of RNA interference (RNAi) reduced nanos2 and boule expression but conversely increased the expression of foxl2. Therefore, our data suggest that Mnnanos2 may serve as a female germ cell marker during gametogenesis and provide chances to uncover its function in adult sea urchin.

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“…Previous studies have focused on the reproductive and developmental biology of L. crocea based on anatomical/histological aspects [6,7], whereas few studies have focused on genes related to spermatogenesis and testicular development. To the best of our knowledge, only a small number of reproduction-related genes have been identified in L. crocea [8][9][10][11]. To further comprehensively explore the molecular mechanisms underlying spermatogenesis and testicular development in L. crocea, large-scale genetic resources must be identified and studied.Recently, RNA-Seq technology has been widely used to explain biological processes in almost all organisms.…”

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confidence: 99%

Transcriptome Sequencing Reveals the Traits of Spermatogenesis and Testicular Development in Large Yellow Croaker (Larimichthys crocea)

Luo

Gao

Ding

et al. 2019

Genes

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Larimichthys crocea is an economically important marine fish in China. To date, the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea have not been thoroughly elucidated. In this study, we conducted a comparative transcriptome analysis between testes (TES) and pooled multiple tissues (PMT) (liver, spleen, heart, and kidney) from six male individuals. More than 54 million clean reads were yielded from TES and PMT libraries. After mapping to the draft genome of L. crocea, we acquired 25,787 genes from the transcriptome dataset. Expression analyses identified a total of 3853 differentially expressed genes (DEGs), including 2194 testes-biased genes (highly expressed in the TES) and 1659 somatic-biased genes (highly expressed in the PMT). The dataset was further annotated by blasting with multi-databases. Functional genes and enrichment pathways involved in spermatogenesis and testicular development were analyzed, such as the neuroactive ligand-receptor interaction pathway, gonadotropin-releasing hormone (GnRH) and mitogen-activated protein kinase (MAPK) signaling pathways, cell cycle pathway, and dynein, kinesin, myosin, actin, heat shock protein (hsp), synaptonemal complex protein 2 (sycp2), doublesex-and mab-3-related transcription factor 1 (dmrt1), spermatogenesis-associated genes (spata), DEAD-Box Helicases (ddx), tudor domain-containing protein (tdrd), and piwi genes. The candidate genes identified by this study lay the foundation for further studies into the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea.Genes 2019, 10, 958 2 of 22 remains for somatic growth; these changes eventually reduce the culture benefit of L. crocea [3] and hinder the conservation of its germplasm resources. Therefore, the molecular mechanisms underlying reproduction regulation must be investigated, and the genes involved in gametogenesis and gonadal development must be identified, to provide a theoretical reference for further studies on the above problems in L. crocea.Directly correlated with sexual maturation and reproduction, in various animal species, including L. crocea, the testis is an essential basic component of the animal reproductive system and responsible for the production of male gametes via spermatogenesis [4]. During the process of spermatogenesis, diploid spermatogonia slowly evolve into many highly specialized spermatozoa through mitosis, meiosis, and spermiogenesis. Stringent spatial and temporal expression of genes during both transcriptional and translational processes are fundamental to ensure the precise processes of spermatogenesis [5]. Previous studies have focused on the reproductive and developmental biology of L. crocea based on anatomical/histological aspects [6,7], whereas few studies have focused on genes related to spermatogenesis and testicular development. To the best of our knowledge, only a small number of reproduction-related genes have been identified in L. crocea [8][9][10][11]. To further comprehensively explore the mol...

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Nanos3 not nanos1 and nanos2 is a germ cell marker gene in large yellow croaker during embryogenesis

Cited by 16 publications

References 54 publications

Primordial germ cells identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.

Primordial germ cells identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.

Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus

Transcriptome Sequencing Reveals the Traits of Spermatogenesis and Testicular Development in Large Yellow Croaker (Larimichthys crocea)

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