Nanophotonic Sialidase Immunoassay for Bacterial Vaginosis Diagnosis

Rodríguez-Nava, Cynthia; Cortés-Sarabia, Karen; Avila-Huerta, Mariana D.; Ortiz-Riaño, Edwin J.; Estrada-Moreno, Ana Karen; Alarcón‐Romero, Luz del Carmen; Mata-Ruíz, Olga; Medina-Flores, Yolanda; Vences-Velázquez, Amalia; Morales‐Narváez, Eden

doi:10.1021/acsptsci.0c00211

Cited by 11 publications

(33 citation statements)

References 27 publications

(49 reference statements)

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“…This nanotechnology has a high sensitivity and specificity (96,29%, respectively). This method offers an original approach to perform a very rapid diagnosis of BV ( Rodríguez-Nava et al., 2021 ).…”

Section: Bacterial Vaginosismentioning

confidence: 99%

Bacterial Vaginosis: What Do We Currently Know?

Chacra

Fénollar

Diop

2022

Front. Cell. Infect. Microbiol.

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The vaginal microbiome is a well-defined compartment of the human microbiome. It has unique conditions, characterized by the dominance of one bacterial species, the Lactobacilli. This microbiota manifests itself by a low degree of diversity and by a strong dynamic of change in its composition under the influence of various exogenous and endogenous factors. The increase in diversity may paradoxically be associated with dysbiosis, such as bacterial vaginosis (BV). BV is the result of a disturbance in the vaginal ecosystem; i.e., a sudden replacement of Lactobacilli by anaerobic bacteria such as Gardnerella vaginalis, Atopobium vaginae, Ureaplasma urealyticum, Mycoplasma hominis, and others. It is the most common cause of vaginal discharge in women of childbearing age, approximately 30% of all causes. The etiology of this dysbiosis remains unknown, but its health consequences are significant, including obstetrical complications, increased risk of sexually transmitted infections and urogenital infections. Its diagnosis is based on Amsel’s clinical criteria and/or a gram stain based on the Nugent score. While both of these methods have been widely applied worldwide for approximately three decades, Nugent score are still considered the “gold standard” of BV diagnostic tools. Given the limitations of these tools, methods based on molecular biology have been developed as alternative rational strategies for the diagnosis of BV. The treatment of BV aims at restoring the balance of the vaginal flora to stop the proliferation of harmful microorganisms. Prescription of antibiotics such as metronidazole, clindamycin, etc. is recommended. Faced with the considerable uncertainty about the cause of BV, the high rate of recurrence, the unacceptable treatment options, and clinical management which is often insensitive and inconsistent, research on this topic is intensifying. Knowledge of its composition and its associated variations represents the key element in improving the therapeutic management of patients with the most suitable treatments possible.

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Section: Bacterial Vaginosismentioning

confidence: 99%

Bacterial Vaginosis: What Do We Currently Know?

Chacra

Fénollar

Diop

2022

Front. Cell. Infect. Microbiol.

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“…For example, real-time photoluminescent biosensing systems targeting protein/enzyme biomarkers and pathogens have been previously developed by using GO-coated microwells. In particular, these platforms employ fluorescence-tagged antibodies as bioprobes which can be quenched in the absence of the analyte. ,, However, as far as we are concerned, recombinant proteins labeled with fluorophores have not been demonstrated to operate as photoluminescent probes targeting antibodies when incubated in GO-coated surfaces. Apart from offering an advantageous platform for the facile determination of COVID-19 seroconversion (circumventing the employment of two bioreagents with capture and detection purposes, blocking and washing steps), this research sheds light in the aforementioned gap in the literature.…”

Section: Discussionmentioning

confidence: 99%

Facile Determination of COVID-19 Seroconversion via Nonradiative Energy Transfer

et al. 2021

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Serological tests are crucial in a pandemic scenario, since they are a valuable tool to spot those citizens with potential immunity, specific regions with herd immunity or particular at-risk populations, as well as acquired immunity after vaccination. Hence, high-throughput, fast, cost-effective, and straightforward technologies facilitating interrogation of COVID-19 seroconversion are an existing need. Herein, we developed an innovative assay for the determination of COVID-19 seroconversion. Fluorophore-labeled SARS-CoV-2 spike receptor-binding domain recombinant protein (F-RBD) was discovered to operate as a bioprobe that emits a strong fluorescence upon COVID-19 antibody detection; however, F-RBD fluorescence was deactivated by graphene oxide-decorated surfaces when COVID-19 antibodies are absent in the sample. With a cost of less than 0.5 USD per test (at laboratory scale), the biosensing system offers optimum results within 42 min. To demonstrate that this technology is technically sound in a relevant environment, 34 human serum samples were analyzed and clearly differentiated, requiring a tiny amount of serum (1 μL to be later diluted in saline buffer).

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“…A score is then assigned to these observations accordingly: 0−3 (normal flora), 4−6 (intermediate or mixed flora), and 7−10 (BV). 11,12 Figure 3A shows the analytical behavior of the analyzed clinical samples. In general, the samples from NM patients exhibited a concentration lower than 25.1 ng mL −1 , and the samples from BV patients displayed a concentration higher than 25.1 ng mL −1 , which is consistent with an immunoassay reported previously.…”

Section: Biosensing Performancementioning

confidence: 99%

“…Therefore, SLD can be employed as a biomarker of BV. 11,12 A common technique for the detection of SLD in vaginal swabs is based on the enzymatic hydrolysis of methoxyphenyl acetyl muramic acid via the catalytic character of SLD, thereby initiating the formation of methoxyphenol, which is then measured. Usually, in this technique, methoxyphenol concentrations greater than 5.1 nM are considered as BV positive.…”

Section: ■ Introductionmentioning

confidence: 99%

“…17 Nevertheless, this test offers a multistep qualitative colorimetric result for the determination of SLD, which eventually leads to different levels of specificity and sensitivity. 11 In this context, our collaborative team engineered a monoclonal antibody that recognizes SLD with high specificity. 18 Herein, as a potential diagnosis tool, we developed a waxprinted μPAD to detect SLD, even in clinical samples.…”

Section: ■ Introductionmentioning

confidence: 99%

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Disposable Device for Bacterial Vaginosis Detection

Avila-Huerta,

Leyva-Hidalgo,

Cortés-Sarabia

et al. 2023

ACS Meas. Sci. Au

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Due to the increasing demand for clinical testing of infectious diseases at the point-of-care, the global market claims alternatives for rapid diagnosis tools such as disposable biosensors, avoiding the need for specialized laboratories and skilled personnel. Bacterial vaginosis (BV) is an infectious disease that commonly affects reproductive-age women and predisposes the infection of sexually transmitted diseases. Especially in asymptomatic cases, BV can lead to pelvic inflammatory conditions, postpartum endometritis, and preterm labor. Conventionally, BV diagnosis involves the microscopic analysis of vaginal swab samples; it thus requires highly trained personnel. In response, we report a novel microfluidic paper-based analytical device for BV diagnosis. Sialidase, a biomarker overexpressed in BV, was detected by exploiting an immunosensing mechanism previously discovered by our team. This technology employs a graphene oxide-coated surface as a quencher of fluorescence; the fluorescence of the immunoprobes that do not experiment immunoreactions (antibody–antigen) are deactivated by graphene oxide via non-radiative energy transfer, whereas those immunoprobes undergoing immunoreactions preserve their photoluminescence due to the distance and the low affinity between the immunocomplex and the graphene oxide-coated surface. Our paper-based test was typically carried out within 20 min, and the sample volume was 6 μL. Besides, it was tested with 14 vaginal swabs specimens to discriminate clinical samples of women with normal microbiota from those with BV. Our disposable device represents a new tool to prevent the consequences of BV.

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Nanophotonic Sialidase Immunoassay for Bacterial Vaginosis Diagnosis

Cited by 11 publications

References 27 publications

Bacterial Vaginosis: What Do We Currently Know?

Bacterial Vaginosis: What Do We Currently Know?

Facile Determination of COVID-19 Seroconversion via Nonradiative Energy Transfer

Disposable Device for Bacterial Vaginosis Detection

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