Nanoparticulate System for the Transdermal Delivery of Catechin as an Antihypercholesterol: In Vitro and In Vivo Evaluations

Abstract

Gambir (Uncaria Gambir, Roxb.) contains catechins that is often empirically used to treat various diseases. Catechins can reduce cholesterol levels by inhibiting coenzyme HMG-CoA reductase that plays a role in cholesterol metabolism. Research has been carried out covering the optimization of transethosomal catechins, the formulation of Transethosomal Catechin Gel (TCG) and Non-Transethosomal Catechin Gel (NTCG), which were then tested for catechin permeation from these gel preparations in vitro using Franz’s diffusion cell with PTFE membranes. The anti-hypercholesterol activity test was carried out with Simvastatin orally as a positive control using 25 male Wistar rats (Rattus norvegicus). The catechin transetosomes have a size of 176.1 ± 5.8 nm, Zeta potential −11.6 ± 5.28, and Entrapment Efficacy of 96.77% ± 0.05. The result of cumulative catechins that permeated from TCG and NTCG were and 172.454 ± 5.287 and 112.741 ± 2.241 μg respectively. Permeation test graphs showed similar permeation and flux profiles. TCG can reduce total cholesterol and LDL (Low Density Lipoprotein) values in rats by 39.77% and 51.52% respectively during 14 days of use.

In Vitro Synergistic Inhibitory Effects of Plant Extract Combinations on Bacterial Growth of Methicillin-Resistant Staphylococcus aureus

“…Glycyrrhizin inhibited the growth of clinical isolates of MRSA and MSSA, with MIC ranging from 32 to 512 µg/mL and 16 to 512 µg/mL, respectively [41]. Catechin (Peak 5) has been reported as the major compound in UG [42]. MIC values of catechin ranged from 78.1 to 156.2 µg/mL against clinical isolates of MRSA [43].…”

Bioprospecting a Film-Forming System Loaded with Eugenia uniflora L. and Tropaeolum majus L. Leaf Extracts for Topical Application in Treating Skin Lesions

“…The receiving compartment was filled with phosphate buffer (pH 7.4) and connected to a bath set at 32 ± 0.5 °C under constant agitation at 100 rpm. The total time of the assay was 8 h, with sampling times (400 µL) of 0.5, 1.0, 2.0, 4.0, 6.0, and 8.0 h, and volume replacement [ 77 ]. The total phenolic compound content was quantified using the Folin–Ciocalteu method [ 78 ].…”

Nanotope™ sebagai Sistem penghantaran Cosmeceutical untuk Meningkatkan Intensitas Efek pada Kulit

“…Stabilitas katekin tergantung pada konsentrasi komponen fenolik. Katekin akan lebih stabil jika memiliki konsentrasi komponen fenolik yang lebih tinggi (Mita et al, 2022).…”