Nanoparticles of two ZnO Precursors as an Encapsulating Matrix of Mangiferin: Associated Studies to Cytotoxic Effects on Liver Cancer Cells Hep-G2 and Healthy Lung Cell Beas-2B

Toxicity of Hybrid Particles (PLGA-ZnO) Loaded with Lupeol and Mangiferina: Ex Vivo Model of Peripheral Cells

“…Regarding mangiferin not inducing apoptosis, as can be seen in Figure , with respect to control cells, these results are in line with studies carried out by our research group on healthy BEAS-2B cell lines, where this phytochemical did not show cytotoxicity; however, it does promote apoptosis in cancer cells (HEPG2) . Like MG, T LP , T MG , and T F 15 showed no induction of apoptosis (Figure ).…”

“…Other studies show that the use of PLGA does not sample damage in erythrocytes at concentrations lower than 200 μg/ mL; 22 however, the use of materials such as ZnO promotes ROS production and consequently eryptosis. 44 According to previous results already mentioned, all the particles have sizes smaller than 300 nm 16,23 and are gastroresistant for 1.5 h (encapsulated with PLGA) 16,22 and 3 h (ZnO); 23,17 for this reason the continuity of this study allows the identificacion materials applicable to the food industry or even to phytopharmacy for polymers such as PLGA. In addition, lupeol is a phytochemical that has been widely studied for its biological properties, 18,20 but its toxicity is concentration-dependent, so encapsulation is an alternative for the development of substances with a potential nutraceutical effect.…”

“…This effect we have described in other publications using ZnO particles loaded with mangiferin and emulsified in PLGA with lupeol . These functionalized particles showed less of a toxic effect compared to zinc oxide particles in HepG2 and BEAS-2, and they showed antitopoisomerase and anticyclooxygenase activity. , …”

“…Two types of ZnO nanoparticles were synthesized by the sol–gel process loaded with mangiferin; , 10 mg of MG/g zinc acetate was used. 2 g of PEG and 10 g of precursor were added, homogenized in 140 mL of ethanol using a magnetic stirrer, and pH was adjusted to 7 with NH 4 OH [1 M], heated with reflux at 70 °C for 3 h, the gel was dried for 24 h at 70 °C and ground to a fine powder, annealed at 500 °C for 5 h under a controlled atmosphere with a heat ramp of 1 °C/min and finally ground again in a mortar to obtain the fine powder called ZnO A -MG. Once these particles were obtained, encapsulation was carried out by the emulsion and solvent evaporation method to obtain hybrid particles (PLGA LP /ZnO A -MG); in the continuous phase 10 mg of PLGA and 1.2 mg of lupeol in acetone were added.…”

“…Before this assay, the treatments were characterized by particle size, , morphological evaluation, ,, release profile, and biological evaluation. ,, …”