Nanoparticles for Neurotrophic Factor Delivery in Nerve Guidance Conduits for Peripheral Nerve Repair

Escobar, Ane; Reis, Rui L.; Oliveira, Joaquím M.

doi:10.2217/nnm-2021-0413

Cited by 10 publications

(9 citation statements)

References 80 publications

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“…Apart from the direct grafting of chemicals to scaffolds, introducing biodegradable chemical-encapsulated nanoparticles into TENGs for local release is a potent strategy to accelerate peripheral nerve repair [150,151]. Tao et al [35] fabricated a hydrogel matrix pipeline comprising GelMA hydrogels and XMU-MP-1-loaded poly (ethylene glycol)poly (3-caprolactone) (MPEG-PCL) nanoparticles by DLPbased technology (figure 5(c) (i)).…”

Section: D-printed Functional Bioengineered Constructs With Chemical ...mentioning

confidence: 99%

3D printing of functional bioengineered constructs for neural regeneration: a review

Zhu

Wei

et al. 2023

Int. J. Extrem. Manuf.

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3D printing technology has opened a new paradigm to controllably and reproducibly fabricate bioengineered neural constructs for potential applications in repairing injured nervous tissues or producing in vitro nervous tissue models. However, the complexity of nervous tissues poses great challenges to three-dimensional (3D)-printed bioengineered analogues, which should possess diverse architectural/chemical/electrical functionalities to resemble the native growth microenvironments for functional neural regeneration. In this work, we provide a state-of-the-art review of the latest development of 3D printing for bioengineered neural constructs. Various 3D printing techniques for neural tissue-engineered scaffolds or living cell-laden constructs are summarized and compared in terms of their unique advantages. We highlight the advanced strategies by integrating topographical, biochemical and electroactive cues inside 3D-printed neural constructs to replicate in vivo-like microenvironment for functional neural regeneration. The typical applications of 3D-printed bioengineered constructs for in vivo repair of injured nervous tissues, bio-electronics interfacing with native nervous system, neural-on-chips as well as brain-like tissue models are demonstrated. The challenges and future outlook associated with 3D printing for functional neural constructs in various categories are discussed.

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Section: D-printed Functional Bioengineered Constructs With Chemical ...mentioning

confidence: 99%

3D printing of functional bioengineered constructs for neural regeneration: a review

Zhu

Wei

et al. 2023

Int. J. Extrem. Manuf.

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“…Other approaches such as using magnesium-based biomaterials and other nanoparticles to promote peripheral nerve regeneration are also under investigation [44 ▪ ,45]. Interesting work is being undertaken to investigate the utility of 4-aminopyridine both to detect axonal continuity and improve functional outcomes after nerve injury [46 ▪ ,47 ▪▪ ,48 ▪ ].…”

Section: Managementmentioning

confidence: 99%

Traumatic peripheral nerve injuries: diagnosis and management

Barnes

Miller²,

Simon

2022

Current Opinion in Neurology

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Purpose of reviewTo review advances in the diagnostic evaluation and management of traumatic peripheral nerve injuries.Recent findingsSerial multimodal assessment of peripheral nerve injuries facilitates assessment of spontaneous axonal regeneration and selection of appropriate patients for early surgical intervention. Novel surgical and rehabilitative approaches have been developed to complement established strategies, particularly in the area of nerve grafting, targeted rehabilitation strategies and interventions to promote nerve regeneration. However, several management challenges remain, including incomplete reinnervation, traumatic neuroma development, maladaptive central remodeling and management of fatigue, which compromise functional recovery.SummaryInnovative approaches to the assessment and treatment of peripheral nerve injuries hold promise in improving the degree of functional recovery; however, this remains a complex and evolving area.

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“…Such type of conduits aims to bridge the injured nerve ends, protects the regeneration from scar tissue formation and guides the regeneration from the proximal to the distal nerve stump [8]. To promote the growth of the axonal tracks, therapeutic stimulants can be added to the conduits, such as GFs or stem cell-based therapies, with Schwann cells often used as the cell of choice [9,10]. The porosity, biocompatibility, stiffness/ flexibility and suturability of the NGCs are key properties to achieve success in PNR [6,7].…”

Section: Introductionmentioning

confidence: 99%

Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration

et al. 2023

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Peripheral nerve injuries represent a major clinical challenge, if nerve ends retract, there is no spontaneous regeneration, and grafts are required to proximate the nerve ends and give continuity to the nerve. The nerve guidance conduits (NGCs) presented in this work are silk fibroin (SF)-based, which is biocompatible and very versatile. The formation of conduits is obtained by forming a covalently cross-linked hydrogel in two concentric moulds, and the inner longitudinally aligned pattern of the SF NGCs is obtained through the use of a patterned inner mould. SF NGCs with two wall thicknesses of ~ 200 to ~ 400 μm are synthesized. Their physicochemical and mechanical characteristics have shown improved properties when the wall thickness is thicker such as resistance to kinking, which is of special importance as conduits might also be used to substitute nerves in flexible body parts. The Young modulus is higher for conduits with inner pattern, and none of the conduits has shown any salt deposition in presence of simulated body fluid, meaning they do not calcify; thus, the regeneration does not get impaired when conduits have contact with body fluids. In vitro studies demonstrated the biocompatibility of the SF NGCs; proliferation is enhanced when iSCs are cultured on top of conduits with longitudinally aligned pattern. BJ fibroblasts cannot infiltrate through the SF wall, avoiding scar tissue formation on the lumen of the graft when used in vivo. These conduits have been demonstrated to be very versatile and fulfil with the requirements for their use in PNR.

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Nanoparticles for Neurotrophic Factor Delivery in Nerve Guidance Conduits for Peripheral Nerve Repair

Cited by 10 publications

References 80 publications

3D printing of functional bioengineered constructs for neural regeneration: a review

3D printing of functional bioengineered constructs for neural regeneration: a review

Traumatic peripheral nerve injuries: diagnosis and management

Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration

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