Nanoparticles based on N-trimethylchitosan: Evaluation of absorption properties using in vitro (Caco-2 cells) and ex vivo (excised rat jejunum) models

Sandri, Giuseppina; Bonferoni, Maria Cristina; Rossi, Silvia; Ferrari, Franca; Gibin, S. R.; Zambito, Ylenia; Colo, Giacomo Di; Caramella, Carla

doi:10.1016/j.ejpb.2006.07.016

Cited by 127 publications

(59 citation statements)

References 26 publications

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“…Chitosan has been shown to have bioadhesive properties due to interactions with the mucous membranes associated with epithelial barriers and tumors (12)(13)(14)(15). Almost all human epithelial cell adenocarcinomas, nonepithelial cancer cell lines, hematological malignancies such as multiple myeloma, and some B-cell non-Hodgkin lymphomas exhibit an over expression of mucin-1 (16).…”

Section: Introductionmentioning

confidence: 99%

“…Almost all human epithelial cell adenocarcinomas, nonepithelial cancer cell lines, hematological malignancies such as multiple myeloma, and some B-cell non-Hodgkin lymphomas exhibit an over expression of mucin-1 (16). Chitosan has been shown to have mucoadhesive properties both in vitro (12,15,17) and in vivo (13,(18)(19)(20)(21). Chitosan and glycerol monooleate (GMO) have been previously co-formulated together to sustain the delivery of PTX in an in situ gel with mucoadhesive properties (22).…”

Section: Introductionmentioning

confidence: 99%

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A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery

2008

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Purpose. To develop a novel nanoparticle drug delivery system consisting of chitosan and glyceryl monooleate (GMO) for the delivery of a wide variety of therapeutics including paclitaxel. Methods. Chitosan/GMO nanoparticles were prepared by multiple emulsion (o/w/o) solvent evaporation methods. Particle size and surface charge were determined. The morphological characteristics and cellular adhesion were evaluated with surface or transmission electron microscopy methods. The drug loading, encapsulation efficiency, in vitro release and cellular uptake were determined using HPLC methods. The safety and efficacy were evaluated by MTT cytotoxicity assay in human breast cancer cells (MDA-MB-231).Results. These studies provide conceptual proof that chitosan/GMO can form polycationic nano-sized particles (400 to 700 nm). The formulation demonstrates high yields (98 to 100%) and similar entrapment efficiencies. The lyophilized powder can be stored and easily be resuspended in an aqueous matrix. The nanoparticles have a hydrophobic inner-core with a hydrophilic coating that exhibits a significant positive charge and sustained release characteristics. This novel nanoparticle formulation shows evidence of mucoadhesive properties; a fourfold increased cellular uptake and a 1000-fold reduction in the IC 50 of PTX. Conclusion. These advantages allow lower doses of PTX to achieve a therapeutic effect, thus presumably minimizing the adverse side effects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery

2008

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“…[26][27][28] Previous studies have shown that chitosan (CS) and TMC NPs are efficient vaccine delivery systems for systemic vaccination. [29][30][31][32] In another study, Slutter et al compared CS and TMC NPs' function in oral immunization. They had indicated that application of TMC NPs is a promising strategy for oral immunization.…”

Section: Discussionmentioning

confidence: 99%

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

Abkar¹,

Fasihi-Ramandi²,

Kooshki³

et al. 2017

IJN

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Brucellosis is a group of closely associated zoonotic bacterial illnesses caused by members of the genus Brucella. B. melitensis Omp31 is a promising candidate for a subunit vaccine against brucellosis. This study surveyed the immunogenicity of Omp31 alone and with incomplete Freund’s adjuvant (Omp31-IFA) and N -trimethyl chitosan (TMC/Omp31) nanoparticles (NPs), as well as the effect of Omp31 immunization route on immunological responses and protection. After expression and purification, the recombinant Omp31 (rOmp31) was loaded onto TMC NPs by ionic gelation. The particle size, loading efficiency and in vitro release of the NPs were examined. Omp31-IFA was administered intraperitoneally, while TMC/Omp31 NPs were administered orally and intraperitoneally. According to the antibody subclasses and cytokine profile, intraperitoneal immunization by Omp31-IFA and TMC/Omp31 NPs induced T helper 1 (Th1) and Th1–Th2 immune responses, respectively. On the other hand, oral immunization with TMC/Omp31 NPs elicited a mixed Th1–Th17 immune response. Data obtained from the cell proliferation assay showed that vaccination with Omp31 stimulated a vigorous antigen-specific cell proliferative response, which could be further increased after oral immunization with TMC/Omp31 NPs. Vaccinated groups of mice when challenged with B. melitensis 16M were found to be significantly protected in the orally administered group in comparison with the intraperitoneally immunized mice. Results of this study indicated that the reason for high protection after oral vaccination can be via elicited Th17 response.

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“…The non-thiolated particles more readily penetrated through the mucus, contacted the underlying epithelial cells and disintegrated. Going one step further, mucoadhesion and tissue permeation of larger 800 nm fluorescent chitosans with different surface chemistries was quantified on isolated rat intestinal mucosa using a Franz diffusion cell (Sandri et al, 2007). Adhesion to the tissue was indirectly quantified from the residual fluorescence in the donor and receiver buffers following exposure.…”

Section: Ex Vivo Tissue Modelsmentioning

confidence: 99%

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Venema²,

et al. 2014

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Engineered metal/mineral, lipid and biochemical macromolecule nanomaterials (NMs) have potential applications in food. Methodologies for the assessment of NM digestion and bioavailability in the gastrointestinal tract are nascent and require refinement. A working group was tasked by the International Life Sciences Institute NanoRelease Food Additive project to review existing models of the gastrointestinal tract in health and disease, and the utility of these models for the assessment of the uptake of NMs intended for food. Gastrointestinal digestion and absorption could be addressed in a tiered approach using in silico computational models, in vitro non-cellular fluid systems and in vitro cell culture models, after which the necessity of ex vivo organ culture and in vivo animal studies can be considered. Examples of NM quantification in gastrointestinal tract fluids and tissues are emerging; however, few standardized analytical techniques are available. Coupling of these techniques to gastrointestinal models, along with further standardization, will further strengthen methodologies for risk assessment.

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Nanoparticles based on N-trimethylchitosan: Evaluation of absorption properties using in vitro (Caco-2 cells) and ex vivo (excised rat jejunum) models

Cited by 127 publications

References 26 publications

A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery

A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

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