Nanoparticles as transfection agents: a comprehensive study with ten different cell lines

Abstract

The performance of transfection agents to deliver nucleic acids into cells strongly depends on the cell type.

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

“…And it seems that the function of DMPC was not compromised even in the cells as DMPC-SPIONs were tending to fuse with the membranes in cells and the nanoparticles were accumulated by cellular organelles. This work encourages further researches related to DMPC-SPIONs being used as high efficient agents for cancer treatment by hyperthermia in external magnetic eld, 31 drug delivery, 32 transfection, 33 and magnetic resonance imaging (MRI) of cells. 34 Cell imaging and tracking is important for the applications such as stem cell therapy.…”

Biological properties of calcium phosphate biomaterials for bone repair: a review

“…77 Osteoblasts and broblasts are generally employed aer receiving the growing factors released by monocytes/macrophages, and play a signicant role in forming new bone tissue. 59,80 Two important properties of an ideal bioactive bone substitution biomaterial are reportedly thought to be exhibiting the same biomechanical competence and regenerating in the same fashion as autologous bone. 73 This requires materials to degrade at the same speed at which osteoblasts lay down new bone on their surfaces, until the material is completely replaced by new, living bone.…”

Poly(lysine) Dendrimers Form Complexes with siRNA and Provide Its Efficient Uptake by Myeloid Cells: Model Studies for Therapeutic Nucleic Acid Delivery

“…The purpose of this step was to compare the toxicity of the obtained dendriplexes with each other and with the cytotoxicity of transfection complexes obtained using the commercially available agent Lipofectamine 2000. This compound, although used as a standard, has been shown to exhibit significant toxicity towards several cell lines, especially during longer incubation times, thus limiting the possibility of its application [45,46]. commercially available agent Lipofectamine 2000.…”