Nanoparticle-mediated delivery of TGF-β1 miRNA plasmid for preventing flexor tendon adhesion formation

Diblock Copolymer Hydrophobicity Facilitates Efficient Gene Silencing and Cytocompatible Nanoparticle-Mediated siRNA Delivery to Musculoskeletal Cell Types

“…39–42 Furthermore, NP have recently been used for drug delivery to tendon, 43 including delivery of TGF- β miRNA plasmids to augment tendon healing by preventing the formation of adhesions. 44 However, to our knowledge, this report is the first to describe NP-mediated delivery of siRNA to tenocytes in vitro.…”

The past, present and future in scaffold-based tendon treatments

“…Recent work investigating the use of injectable poly(lactic-co-glycolic acid) (PLGA) nanoparticles to transfect intrinsic cells in vivo with TGF-β in a chicken foot induced defect model demonstrated sustained delivery for over a 4 week period, leading to a small, but significant reduction in adhesion formation. However a large significant reduction in tendon mechanical properties was recorded [207], demonstrating that while some aspects of tendon healing can be improved by using this method, further work is needed to enable overall therapeutic benefits. Although gene therapy has great potential in difficult to cure injuries and degenerative conditions (e.g.…”

Nanoparticles for Tendon Healing and Regeneration: Literature Review

“…Nanotechnology has developed several nanoparticles to be used for gene therapy to replace the viral vectors and avoid their side effects (Raffa et al, 2011). Zhou et al evaluated the use of nanoparticles as non-viral vector for gene therapy to prevent peritendineus adhesion formation (Zhou et al, 2013). The authors reported that the miRNAs reducing the expression of TGF-b1 (induce fibrotic changes and adhesion formations in the tissues like tendons) were inserted into the plasmid, and then the generated plasmids were loaded into PEI-polylactic-co-glycolic acid (PLGA) nanoparticles for preventing peritendinous adhesion (Zhou et al, 2013).…”

“…Zhou et al evaluated the use of nanoparticles as non-viral vector for gene therapy to prevent peritendineus adhesion formation (Zhou et al, 2013). The authors reported that the miRNAs reducing the expression of TGF-b1 (induce fibrotic changes and adhesion formations in the tissues like tendons) were inserted into the plasmid, and then the generated plasmids were loaded into PEI-polylactic-co-glycolic acid (PLGA) nanoparticles for preventing peritendinous adhesion (Zhou et al, 2013). Interestingly, they performed their study in vitro , using a culture of primary tenocites from flexor digitorum profundus tendon of Leghorn chicken, and in vivo by using the same type of tendon.…”

“…The analysis of the data obtained by using electron microscopy imaging, molecular biology techniques, and biomechanical tests, underline the potential of PLGA nanoparticles as an innovative and efficient agent for gene delivery in tendon. In fact, the transfection of miRNA by PLGA nanoparticles resulted in an inhibition of TGF-b1 expressions, which in turn induced the repair of the tendon (Zhou et al, 2013). The results of this study showed the strength of the treated tendons was lower of than in the control group due to the down regulation of cells migration, proliferation, adherence, apoptosis, and of the secretion of ECM related to the inhibition og TGF-b1 (Zhou et al, 2013).…”