Nanoparticle‐Loaded Protein–Polymer Nanodroplets for Improved Stability and Conversion Efficiency in Ultrasound Imaging and Drug Delivery

Lee, Jeong Yu; Carugo, Dario; Crake, Calum; Owen, Joshua; Victor, Marie de Saint; Seth, Anjali; Coussios, Constantin C.; Stride, Eleanor

doi:10.1002/adma.201502022

Cited by 125 publications

(98 citation statements)

References 42 publications

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“…[9] Amphiphilic chitosan oligosaccharide and deoxycolic acid (COSD) were synthesized by a coupling reaction of succinimido deoxycholic acids to the primary amine group of chitosan chains [22] (Figure S1, Supporting Information). COSD nanoparticles (CPs) incorporating magnetic nanoparticles in their core were prepared by an oil-in-water emulsification method.…”

Section: Methodsmentioning

confidence: 99%

“…Details of this device have been published previously [9] but briefly the resonator consists of a piezoelectric transducer, a carrier layer which couples the acoustic energy to the other components of the device, a fluid layer with nanodroplets in suspension, and a reflector layer which reflects the acoustic energy back into the device. The experimentally measured resonance frequency for the device was ≈1.85 MHz, in agreement with computational predictions.…”

Section: Methodsmentioning

confidence: 99%

“…[14] However, the theoretical prediction did not take into account the additional surface pressure produced by the shell, coalescence of individual bubbles in close proximity to each other, or absorption of dissolved gases from the phosphate buffered saline (PBS). [9,15] Moreover, there may have been an effect of temperature rise over the course of the experiment, although the maximum liquid temperature measured after 3 min of continuous wave ultrasound exposure was only ≈42 °C ( Figure S6, Supporting Information).…”

Section: Particle Characteristicsmentioning

confidence: 99%

“…[9] Figure 4a-c shows maps of summed cavitation energy overlaid on B-mode images from each experiment. When water was exposed to ultrasound in the absence of any particles, the maximum PAM signal was 79.26 energy units (please see the Experimental Section) and the energy distribution was comparatively uniform.…”

Section: Acoustic Emissionsmentioning

confidence: 99%

“…[8] We have previously demonstrated that both the stability and phase conversion efficiency of nanodroplets can be considerably improved by the inclusion of iron oxide nanoparticles. [9] The resulting droplets were able to enhance the delivery of a conventional chemotherapy drug, paclitaxel, generating a 40% improvement in cytotoxicity compared with free drug.…”

mentioning

confidence: 99%

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Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Lee

Crake

Teo

et al. 2017

Adv Healthcare Materials

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Small interfering RNA (siRNA) has significant therapeutic potential but its clinical translation has been severely inhibited by a lack of effective delivery strategies. Previous work has demonstrated that perfluorocarbon nanodroplets loaded with magnetic nanoparticles can facilitate the intracellular delivery of a conventional chemotherapeutic drug. The aim of this study is to determine whether a similar agent can provide a means of delivering siRNA, enabling efficient transfection without degradation of the molecule. Chitosandeoxycholic acid nanoparticles containing perfluoropentane and iron oxide (d 0 = 7.5 ± 0.35 nm) with a mean hydrodynamic diameter of 257.6 ± 10.9 nm are produced. siRNA (AllStars Hs cell death siRNA) is electrostatically bound to the particle surface and delivery to lung cancer cells and breast cancer cells is investigated with and without ultrasound exposure (500 kHz, 1 MPa peakto-peak focal pressure, 40 cycles per burst, 1 kHz pulse repetition frequency, 10 s duration). The results show that siRNA functionality is not impaired by the treatment protocol and that the nanodroplets are able to successfully promote siRNA uptake, leading to significant apoptosis (52.4%) 72 h after ultrasound treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Particle Characteristicsmentioning

confidence: 99%

Section: Acoustic Emissionsmentioning

confidence: 99%

mentioning

confidence: 99%

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Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Lee

Crake

Teo

et al. 2017

Adv Healthcare Materials

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A Multi‐Gradient Targeting Drug Delivery System Based on RGD‐l‐TRAIL‐Labeled Magnetic Microbubbles for Cancer Theranostics

Duan

Yang

et al. 2016

Adv Funct Materials

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The accurately and efficiently targeted delivery of therapeutic/diagnostic agents into tumor areas in a controllable fashion remains a big challenge. Here, a novel cancer targeting magnetic microbubble is elaborately fabricated. First, the γ-Fe 2 O 3 magnetic iron oxide nanoparticles are optimized to chemically conjugate on the surface of polymer microbubbles. Then, arginineglycine-aspartic acid-l-tumor necrosis factor-related apoptosis-inducing ligand (RGD-l-TRAIL), antitumor targeting fusion protein, is precisely conjugated with magnetic nanoparticles of microbubbles to construct RGD molecularly targeted magnetic microbubble, which is defined as RGD-l-TRAIL@MMBs. Such RGD-l-TRAIL@MMBs is endowed with the multigradient cascade targeting ability following by magnetic targeting, RGD, as well as enhanced permeability and retention effect regulated targeting to result in high cancerous tissue targeting efficiency. Due to the highly specific accumulation of RGD-l-TRAIL@MMBs in the tumor, the accurate diagnostic information of tumor can be obtained by dual ultrasound and magnetic resonance imaging. After imaging, the TRAIL molecules as anticancer agent also get right into the cancer cells by nanoparticle-and RGD-mediated endocytosis to effectively induce the tumor cell apoptosis. Therefore, RGD-l-TRAIL conjugated magnetic microbubbles could be developed as a molecularly targeted multimodality imaging delivery system with the addition of chemotherapeutic cargoes to improve cancer diagnosis and therapy.

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Ultrasound Activatable Microbombs via Co‐Loading CaO₂/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention

Guo

Liu

Lei

et al. 2023

Adv Funct Materials

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Biofilms are the chief culprits of most intractable infections and pose great threat to human health. Developing effective strategies to sweep away the detrimental microbe and stubborn biofilm still remains a challenge. Here, an ultrasound (US) activated microbomb is constructed by encapsulating perfluorohexane (PFH) and iron-tannin modified calcium peroxide (CaO 2 -TA-Fe) in poly(lactide-co-glycolide) (PLGA) vesicles as the reactive oxygen species (ROS) generating reactors for dental biofilm elimination. Upon the US irradiation by ultrasonic toothbrush, the swiftly vaporized PFH can not only promptly explode the PLGA shell to facilitate the generation of H 2 O 2 from CaO 2 , but also blast the tight biofilm for effective delivery of the lethal hydroxyl free radicals (•OH) caused by degradative Fe 3+ from iron-tannin network, thereby proceeding cascade catalysis eradication of biofilm. As a proofof-concept, this study demonstrates the proposed synergetic paradigm that integrates the US-triggered explosion with ROS-induced sterilization for stubborn biofilm elimination, and is a promising tactic for the costly and prevalent caries prevention as well as the biofilm-associated diseases treatments.

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Nanoparticle‐Loaded Protein–Polymer Nanodroplets for Improved Stability and Conversion Efficiency in Ultrasound Imaging and Drug Delivery

Cited by 125 publications

References 42 publications

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

A Multi‐Gradient Targeting Drug Delivery System Based on RGD‐l‐TRAIL‐Labeled Magnetic Microbubbles for Cancer Theranostics

Ultrasound Activatable Microbombs via Co‐Loading CaO₂/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention

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Nanoparticle‐Loaded Protein–Polymer Nanodroplets for Improved Stability and Conversion Efficiency in Ultrasound Imaging and Drug Delivery

Cited by 125 publications

References 42 publications

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

Ultrasound‐Enhanced siRNA Delivery Using Magnetic Nanoparticle‐Loaded Chitosan‐Deoxycholic Acid Nanodroplets

A Multi‐Gradient Targeting Drug Delivery System Based on RGD‐l‐TRAIL‐Labeled Magnetic Microbubbles for Cancer Theranostics

Ultrasound Activatable Microbombs via Co‐Loading CaO2/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention

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Product

Resources

About

Ultrasound Activatable Microbombs via Co‐Loading CaO₂/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention