Nanoparticle-induced tight-junction opening for the transport of an anti-angiogenic sulfated polysaccharide across Caco-2 cell monolayers

Yu, Shu Huei; Tang, Deh Wei; Hsieh, Hao Ying; Wu, Wen‐Hsin; Lin, Bo; Chuang, Er-Tuan; Sung, Hsing‐Wen; Mi, Fwu Long

doi:10.1016/j.actbio.2013.04.009

Cited by 70 publications

(38 citation statements)

References 41 publications

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“…The pKa values of sulfate esters in sulfated fucose are around 1.5 while the pKa value of the carboxylate group in glucuronic acid is about 3.0. At pH 2.0, strong acid protonated the carboxylate ions (-COO − ) in fucoidan, the polyelectrolyte complex of chitosan and fucoidan thus could be rapidly broken down [28]. After chemical modification, the carboxylate group decreased in amount but the sulfonate group increased due to the conjugation of taurine with the glucuronic acid residue of fucoidan.…”

Section: Resultsmentioning

confidence: 99%

“…The nanoparticles were usually prepared by adding polyanions into excess amounts of chitosan solution to obtain nanoparticles covered with positively charged chitosan. In recent years, increased attention has been focused on the development of chitosan/fucoidan (CS/FD) complex nanoparticles for drug delivery [24,25,26,27,28,29,30]. Our previous study developed a chitosan/fucoidan (FD) nanoparticle with chitosan dominant at an outer layer.…”

Section: Introductionmentioning

confidence: 99%

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Delivery of Berberine Using Chitosan/Fucoidan-Taurine Conjugate Nanoparticles for Treatment of Defective Intestinal Epithelial Tight Junction Barrier

et al. 2014

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Bacterial-derived lipopolysaccharides (LPS) can cause defective intestinal barrier function and play an important role in the development of inflammatory bowel disease. In this study, a nanocarrier based on chitosan and fucoidan was developed for oral delivery of berberine (Ber). A sulfonated fucoidan, fucoidan-taurine (FD-Tau) conjugate, was synthesized and characterized by Fourier transform infrared (FTIR) spectroscopy. The FD-Tau conjugate was self-assembled with berberine and chitosan (CS) to form Ber-loaded CS/FD-Tau complex nanoparticles with high drug loading efficiency. Berberine release from the nanoparticles had fast release in simulated intestinal fluid (SIF, pH 7.4), while the release was slow in simulated gastric fluid (SGF, pH 2.0). The effect of the berberine-loaded nanoparticles in protecting intestinal tight-junction barrier function against nitric oxide and inflammatory cytokines released from LPS-stimulated macrophage was evaluated by determining the transepithelial electrical resistance (TEER) and paracellular permeability of a model macromolecule fluorescein isothiocyanate-dextran (FITC-dextran) in a Caco-2 cells/RAW264.7 cells co-culture system. Inhibition of redistribution of tight junction ZO-1 protein by the nanoparticles was visualized using confocal laser scanning microscopy (CLSM). The results suggest that the nanoparticles may be useful for local delivery of berberine to ameliorate LPS-induced intestinal epithelia tight junction disruption, and that the released berberine can restore barrier function in inflammatory and injured intestinal epithelial.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Delivery of Berberine Using Chitosan/Fucoidan-Taurine Conjugate Nanoparticles for Treatment of Defective Intestinal Epithelial Tight Junction Barrier

et al. 2014

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“…The preparation of fucoidan-chitosan nanoparticles can be achieved by the ionic crosslinking method due to ionic bonds linking one polymer chain to another. In this procedure, solutions of fucoidan are added to chitosan solutions by flush mixing using a pipette tip with ultrasonic vibration in an ice bath [120][121][122][123]. Through this technique, it is also possible to achieve entrapment efficiencies of 90%, with sizes up to 500 nm due to a wider entangling of the molecular chains.…”

Section: Preparation Methods Of Fucoidan-chitosan Nanoparticlesmentioning

confidence: 99%

“…However, the pH-responsive profile of fucoidan-chitosan nanoparticles prevents degradation under acidic conditions of the gastrointestinal tract and allows drug absorption in the intestine. Hence, fucoidan-chitosan nanoparticles have been widely explored for oral delivery of active pharmaceutical ingredients [110,111,113,118,[123][124][125][126][127][128][129], also taking advantage of chitosan's property of increased contact with the mucus layer and consequently longer resistance times at the absorption site [129]. The biological properties of fucoidan described in Section 3.1.1 were explored by some authors to obtain synergic effects with a drug, for example, ciprofloxacin [117] or gemcitabine [126].…”

Section: Nanomedicine Applications Of Fucoidan-chitosan Nanoparticlesmentioning

confidence: 99%

Marine Polysaccharides in Pharmaceutical Applications: Fucoidan and Chitosan as Key Players in the Drug Delivery Match Field

et al. 2019

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The use of marine-origin polysaccharides has increased in recent research because they are abundant, cheap, biocompatible, and biodegradable. These features motivate their application in nanotechnology as drug delivery systems; in tissue engineering, cancer therapy, or wound dressing; in biosensors; and even water treatment. Given the physicochemical and bioactive properties of fucoidan and chitosan, a wide range of nanostructures has been developed with these polysaccharides per se and in combination. This review provides an outline of these marine polysaccharides, including their sources, chemical structure, biological properties, and nanomedicine applications; their combination as nanoparticles with descriptions of the most commonly used production methods; and their physicochemical and biological properties applied to the design of nanoparticles to deliver several classes of compounds. A final section gives a brief overview of some biomedical applications of fucoidan and chitosan for tissue engineering and wound healing.

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“…Enterocytes constitute the major part of intestinal epithelial cells. The most commonly used in vitro model for intestinal cells is the human Caco-2 cell line (Gitrowski et al, 2014;He et al, 2013;Hubatsch et al, 2007;Lampen et al, 2004;Yu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanism of silver nanoparticles in human intestinal cells

et al. 2015

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Silver nanoparticles are used in consumer products like food contact materials, drinking water technologies and supplements, due to their antimicrobial properties. This leads to an oral uptake and exposure of intestinal cells. In contrast to other studies we found no apoptosis induction by surfactant-coated silver nanoparticles in the intestinal cell model Caco-2 in a previous study, although the particles induced oxidative stress, morphological changes and cell death. Therefore, this study aimed to analyze the molecular mechanism of silver nanoparticles in Caco-2 cells. We used global gene expression profiling in differentiated Caco-2 cells, supported by verification of the microarray data by quantitative real-time RT-PCR and microscopic analysis, impedance measurements and assays for apoptosis and oxidative stress. Our results revealed that surfactant-coated silver nanoparticles probably affect the cells by outside-in signaling. They induce oxidative stress and have an influence on canonical pathways related to FAK, ILK, ERK, MAPK, integrins and adherence and tight junctions, thereby inducing transcription factors like AP1, NFkB and NRF2, which mediate cellular reactions in response to oxidative stress and metal ions and induce changes in the cytoskeleton and cell-cell and cell-matrix contacts. The present data confirm the absence of apoptotic cell death. Non-apoptotic, necrotic cell death, especially in the intestine, can cause inflammation and influence the mucosal immune response.

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Nanoparticle-induced tight-junction opening for the transport of an anti-angiogenic sulfated polysaccharide across Caco-2 cell monolayers

Cited by 70 publications

References 41 publications

Delivery of Berberine Using Chitosan/Fucoidan-Taurine Conjugate Nanoparticles for Treatment of Defective Intestinal Epithelial Tight Junction Barrier

Delivery of Berberine Using Chitosan/Fucoidan-Taurine Conjugate Nanoparticles for Treatment of Defective Intestinal Epithelial Tight Junction Barrier

Marine Polysaccharides in Pharmaceutical Applications: Fucoidan and Chitosan as Key Players in the Drug Delivery Match Field

Molecular mechanism of silver nanoparticles in human intestinal cells

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