Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing

Kasas, Sandor; Malovichko, Anton; Villalba, María Inés; Vela, María Elena; Yantorno, Osvaldo; Willaert, Ronnie

doi:10.3390/antibiotics10030287

Cited by 22 publications

(15 citation statements)

References 151 publications

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“…Numerous studies demonstrated that living organisms attached to AFM cantilevers induce oscillations of the lever that immediately stop when the organism dies [23,24]. Hence, this newly developed technology, called cellular nanomotion detection (NMD), detects living organisms in a chemistry-independent manner and offers a clear advantage in terms of test velocity [25,26], but possesses several drawbacks too. AFMs, unfortunately, are expensive and relatively complex devices that require some expertise to operate.…”

Section: Introductionmentioning

confidence: 99%

The Dynamics of Single-Cell Nanomotion Behaviour of Saccharomyces cerevisiae in a Microfluidic Chip for Rapid Antifungal Susceptibility Testing

et al. 2022

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The fast emergence of multi-resistant pathogenic yeasts is caused by the extensive—and sometimes unnecessary—use of broad-spectrum antimicrobial drugs. To rationalise the use of broad-spectrum antifungals, it is essential to have a rapid and sensitive system to identify the most appropriate drug. Here, we developed a microfluidic chip to apply the recently developed optical nanomotion detection (ONMD) method as a rapid antifungal susceptibility test. The microfluidic chip contains no-flow yeast imaging chambers in which the growth medium can be replaced by an antifungal solution without disturbing the nanomotion of the cells in the imaging chamber. This allows for recording the cellular nanomotion of the same cells at regular time intervals of a few minutes before and throughout the treatment with an antifungal. Hence, the real-time response of individual cells to a killing compound can be quantified. In this way, this killing rate provides a new measure to rapidly assess the susceptibility of a specific antifungal. It also permits the determination of the ratio of antifungal resistant versus sensitive cells in a population.

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Section: Introductionmentioning

confidence: 99%

The Dynamics of Single-Cell Nanomotion Behaviour of Saccharomyces cerevisiae in a Microfluidic Chip for Rapid Antifungal Susceptibility Testing

et al. 2022

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“…Like direct AST, the AtbFinder system enables direct sampling of biological specimens without the need for culturing or time-consuming sample processing. Furthermore, the AtbFinder method can indicate suitable antibiotics in only 4 h, whereas direct AST requires 18–36 h (and that is why direct AST is not applicable for tailoring empirical antibiotic therapy) [ 86 , 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a New Culture-Based AtbFinder Test-System Employing a Novel Nutrient Medium for the Selection of Optimal Antibiotics for Critically Ill Patients with Polymicrobial Infections within 4 h

Tetz¹,

Tetz²

2021

Microorganisms

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Here, we describe the validation of a new phenotypic culture-based AtbFinder method for rapid selection of antibiotics in vitro using specimens with mono- and polybacterial infections. AtbFinder, which can be applied to any type of non-blood tissue, does not require isolation of pure bacterial cultures. The method uses a novel TGV medium that allows more rapid bacterial growth of Gram-positive and Gram-negative monoisolates compared with that achieved with conventional laboratory media, demonstrating overall sensitivity, specificity, PPV, NPV values of 99.6%, 98.1%, 98.5%, and 99.4%, respectively, after 4 h. For polymicrobial infections, AtbFinder utilized a novel paradigm of the population response to antibiotics, enabling bacterial growth in the form of a mixed microbial community and selecting antibiotics targeting not only the principal pathogen, but also those bacteria that support their growth. TGV medium allowed culturing of a more diverse set of bacteria from polymicrobial biospecimens, compared with that achieved with the standard media, and enabled, within 4 h, accurate selection of the antibiotics that completely eliminated all cultivatable bacteria from clinical samples. In conclusion, the AtbFinder system may be a valuable tool in improving antibiotic selection, and enabling targeted empirical therapy and accurate antibiotic replacement, which is especially important in high-risk patients.

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“…Mechanical sensors have been proposed for AST [76,98]. The forces produced by the interaction of the target species with the receptors immobilized on the cantilever determine a change in the cantilever resonance frequency that behaves as a harmonic oscillator.…”

Section: Ast Magnetic Mass and Mechanical (Bio)sensorsmentioning

confidence: 99%

“…For the determination of bacteria and their susceptibility or resistance, the cells adhere to the cantilever, and according to the added mass and position, the change in resonance frequency is monitored [74,99,100]. Currently, the atomic force microscope (AFM) is one of the main nanomechanical techniques used in AST due to its ability to detect displacements in the range of 0.1 Å and a resolution of microseconds [98,101]. Using an AFM system, Stupar et al developed a methodology capable of detecting nanomotion of E. coli bacteria and observing their behavior when exposed to ciprofloxacin, ampicillin, and ceftriaxone [102].…”

Section: Ast Magnetic Mass and Mechanical (Bio)sensorsmentioning

confidence: 99%

Advances in Antimicrobial Resistance Monitoring Using Sensors and Biosensors: A Review

et al. 2021

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The indiscriminate use and mismanagement of antibiotics over the last eight decades have led to one of the main challenges humanity will have to face in the next twenty years in terms of public health and economy, i.e., antimicrobial resistance. One of the key approaches to tackling antimicrobial resistance is clinical, livestock, and environmental surveillance applying methods capable of effectively identifying antimicrobial non-susceptibility as well as genes that promote resistance. Current clinical laboratory practices involve conventional culture-based antibiotic susceptibility testing (AST) methods, taking over 24 h to find out which medication should be prescribed to treat the infection. Although there are techniques that provide rapid resistance detection, it is necessary to have new tools that are easy to operate, are robust, sensitive, specific, and inexpensive. Chemical sensors and biosensors are devices that could have the necessary characteristics for the rapid diagnosis of resistant microorganisms and could provide crucial information on the choice of antibiotic (or other antimicrobial medicines) to be administered. This review provides an overview on novel biosensing strategies for the phenotypic and genotypic determination of antimicrobial resistance and a perspective on the use of these tools in modern health-care and environmental surveillance.

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Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing

Cited by 22 publications

References 151 publications

The Dynamics of Single-Cell Nanomotion Behaviour of Saccharomyces cerevisiae in a Microfluidic Chip for Rapid Antifungal Susceptibility Testing

The Dynamics of Single-Cell Nanomotion Behaviour of Saccharomyces cerevisiae in a Microfluidic Chip for Rapid Antifungal Susceptibility Testing

Evaluation of a New Culture-Based AtbFinder Test-System Employing a Novel Nutrient Medium for the Selection of Optimal Antibiotics for Critically Ill Patients with Polymicrobial Infections within 4 h

Advances in Antimicrobial Resistance Monitoring Using Sensors and Biosensors: A Review

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