Nanomembrane‐Based, Thermal‐Transport Biosensor for Living Cells

ElAfandy, Rami T.; Abuelela, Ayman F.; Mishra, Pawan; Janjua, Bilal; Oubei, Hassan M.; Büttner, U.; Majid, Mohammed Abdul; Ng, Tien Khee; Merzaban, Jasmeen S.; Ooi, Boon S.

doi:10.1002/smll.201603080

Cited by 21 publications

(20 citation statements)

References 42 publications

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“…nanomembranes were slightly less than that of water 61 . We explain the difference by pointing out that both groups have provided heat to the specimen from outside of the cells.…”

Section: Discussionmentioning

confidence: 76%

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

Sotoma

Zhong

Kah

et al. 2020

Preprint

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Understanding heat dissipation processes at nanoscale during cellular thermogenesis is essential to clarify the relationships between the heat and biological processes in cells and organisms. A key parameter determining the heat flux inside a cell is the local thermal conductivity, a factor poorly investigated both experimentally and theoretically. Here, using a nanoheater/nanothermometer hybrid based on a polydopamine shell encapsulating a fluorescent diamond nanocrystal, we measured the intracellular thermal conductivity of HeLa cell with a spatial resolution of about 200 nm. Its mean value of 0.11 Wm -1 K -1 determined for the first time is significantly smaller than that of water. Bayesian analysis of the data strongly supports the existence of variation of the intracellular thermal conductivity of about 40%. These results present a major milestone towards understanding the intracellular heat transfer phenomena at nanoscale.

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“…nanomembranes were slightly less than that of water 61 . We explain the difference by pointing out that both groups have provided heat to the specimen from outside of the cells.…”

Section: Discussionmentioning

confidence: 76%

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

Sotoma

Zhong

Kah

et al. 2020

Preprint

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“…The temperature change occurred over a length scale of ~50 μm (Δ x ) along the length of the probe that has a cross-sectional area (~5 μm 2 ). We can assume that temperature isotherms are spherically symmetric inside the cell given the similarity in the thermal conductivity ( k ) of the cell (~0.6 Wm −1 K −1 ) 45 and the probe tip ( k SiNx ~0.8 Wm −1 K −1 ) 46 , respectively. This leads to an estimated (initial) heat flow through the probe of ~400 nW.…”

Section: Discussionmentioning

confidence: 99%

Transient heat release during induced mitochondrial proton uncoupling

et al. 2019

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Non-shivering thermogenesis through mitochondrial proton uncoupling is one of the dominant thermoregulatory mechanisms crucial for normal cellular functions. The metabolic pathway for intracellular temperature rise has widely been considered as steady-state substrate oxidation. Here, we show that a transient proton motive force (pmf) dissipation is more dominant than steady-state substrate oxidation in stimulated thermogenesis. Using transient intracellular thermometry during stimulated proton uncoupling in neurons of Aplysia californica , we observe temperature spikes of ~7.5 K that decay over two time scales: a rapid decay of ~4.8 K over ~1 s followed by a slower decay over ~17 s. The rapid decay correlates well in time with transient electrical heating from proton transport across the mitochondrial inner membrane. Beyond ~33 s, we do not observe any heating from intracellular sources, including substrate oxidation and pmf dissipation. Our measurements demonstrate the utility of transient thermometry in better understanding the thermochemistry of mitochondrial metabolism.

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“…El Afandy et al described a nanomembrane-based sensor of thermal diffusivity and conductivity [18]. Their technique is based on the increase of phonon-boundary-scattering rate of nanomembranes.…”

Section: Biosensorsmentioning

confidence: 99%

“…Some particular application examples include wastewater treatment [7], desalination [8,9], removal of toxic chemicals [10], of bacterial and viral agents [11] and gathering and concentration of targeted species (e.g., precious metals) from seawater [12]. Another wide field of use is biomedicine and life sciences [13], and covers such diverse areas as biointerfaces including brain-machine interfaces [14], scaffolds for tissue growth [15,16], biosensors [17,18], drug delivery and targeting [19], various labs-on-a-chip [20], DNA sequencers [21], etc. A field where the benefits of biomimetic nanomembranes are already starting to lead to large advancements is renewable energy [22].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Nanomembranes: An Overview

Jakšić

2020

Biomimetics

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Nanomembranes are the principal building block of basically all living organisms, and without them life as we know it would not be possible. Yet in spite of their ubiquity, for a long time their artificial counterparts have mostly been overlooked in mainstream microsystem and nanosystem technologies, being a niche topic at best, instead of holding their rightful position as one of the basic structures in such systems. Synthetic biomimetic nanomembranes are essential in a vast number of seemingly disparate fields, including separation science and technology, sensing technology, environmental protection, renewable energy, process industry, life sciences and biomedicine. In this study, we review the possibilities for the synthesis of inorganic, organic and hybrid nanomembranes mimicking and in some way surpassing living structures, consider their main properties of interest, give a short overview of possible pathways for their enhancement through multifunctionalization, and summarize some of their numerous applications reported to date, with a focus on recent findings. It is our aim to stress the role of functionalized synthetic biomimetic nanomembranes within the context of modern nanoscience and nanotechnologies. We hope to highlight the importance of the topic, as well as to stress its great applicability potentials in many facets of human life.

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Nanomembrane‐Based, Thermal‐Transport Biosensor for Living Cells

Cited by 21 publications

References 42 publications

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

Transient heat release during induced mitochondrial proton uncoupling

Biomimetic Nanomembranes: An Overview

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