Nanomedicine: The Promise and Challenges in Cancer Chemotherapy

Naguib, Youssef W.; Cui, Zhengrong

doi:10.1007/978-94-017-8739-0_11

Cited by 24 publications

(25 citation statements)

References 223 publications

(138 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uptake of the nanoparticles was observed using an optical microscope (BX51; Olympus Corp., Tokyo, Japan) following hematoxylin staining for 1 min at room temperature. 5 and 1x10 5 cells/well in a 6-well culture plate, respectively) were mixed and co-cultured at 37˚C under 5% CO 2 . After 2 days, the magnetite nanoparticles were added to the culture medium.…”

Section: Uptake Of Magnetite Nanoparticles By Human Macrophages and Tmentioning

confidence: 99%

See 1 more Smart Citation

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Komohara

Kawauchi

Makiyama

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Previous studies have indicated pro-tumor functions of macrophages in tumor progression in different types of malignant tumors. The detailed mechanisms of cell-cell interaction between macrophages and tumor cells have been investigated by means of in vitro co-culture experiments. The present study developed magnetite nanoparticles modified with gelatin that are specifically engulfed by macrophages and investigated methods to deplete these macrophages in co-culture experiments using a magnet. T98G glioma cell line and human monocyte-derived macrophages were mixed and co-cultured for 2 days. The T98G cells were isolated by depletion of the macrophages using the magnetite nanoparticles. mRNA expression of a number of pro-tumor molecules in the isolated T98G cells, with or without co-culture with macrophages, was then evaluated. The mRNA expression levels of chemokine (CC motif) ligand 2, interleukin-6 and macrophage-colony stimulating factor receptor (M-CSFR) were significantly upregulated in T98G cells by co-culture with macrophages (P<0.01). M-CSFR protein expression was also increased by co-culture with macrophages. The conditioned medium of co-cultured cells increased M-CSFR expression in T98G cells. Magnetite nanoparticles may be a novel tool not only for investigating the unique activation status of tumor cells in co-culture conditions, but also for targeting pro-tumor macrophages in tumor tissues.

show abstract

Section: Uptake Of Magnetite Nanoparticles By Human Macrophages and Tmentioning

confidence: 99%

“…Since TAMs have pro-tumor functions in a number of malignant tumor types, TAMs are also considered as target cells for anti-tumor therapy. Previously, different materials, such as nanoparticles and nanocarriers, have been reported to improve anti-tumor therapy (5,6).…”

Section: Introductionmentioning

confidence: 99%

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Komohara

Kawauchi

Makiyama

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Nanoparticle size, shape, and surface characteristics (particularly charge and hydrophobicity) can influence the cellular uptake pathways. 36 LDH nanoparticles, which are positively charged, can enter cells through the clathrin-mediated endocytotic pathway. 14,21,22 Negatively charged PEG-PLDH nanoparticles, however, may interact with cationic lipids (like sphingomyelin whose polar domain contains an amine group) on the cell membrane and then become internalized via caveolae-dependent endocytosis.…”

Section: Cellular Uptake In Hela Cellsmentioning

confidence: 99%

Improvement of pharmacokinetic and antitumor activity of layered double hydroxide nanoparticles by coating with PEGylated phospholipid membrane

Yan¹,

Zhang²,

Sheng-miao³

et al. 2014

IJN

View full text Add to dashboard Cite

Layered double hydroxide (LDH) has attracted considerable attention as a drug carrier. However, because of its poor in vivo behavior, polyethylene glycolylated (PEGylated) phospholipid must be used as a coformer to produce self-assembled core–shell nanoparticles. In the present study, we prepared a PEGylated phospholipid-coated LDH (PLDH) (PEG-PLDH) delivery system. The PEG-PLDH nanoparticles had an average size of 133.2 nm. Their core–shell structure was confirmed by transmission electron microscopy and X-ray photoelectron spectroscopy. In vitro liposome-cell-association and cytotoxicity experiments demonstrated its ability to be internalized by cells. In vivo studies showed that PEGylated phospholipid membranes greatly reduced the blood clearance rate of LDH nanoparticles. PEG-PLDH nanoparticles demonstrated a good control of tumor growth and increased the survival rate of mice. These results suggest that PEG-PLDH nanoparticles can be a useful drug delivery system for cancer therapy.

show abstract

“…23 Mechanisms of resistance to alkylating agents mainly involve O 6 -methylguanine methyltransferase (MGMT), DNA mismatch repair (MMR) pathway, and base excision repair (BER) pathway. One important mechanism of resistance to alkylating agents is mediated by the DNA repair enzyme MGMT, which repairs O 6 -methylguanine adducts.…”

mentioning

confidence: 99%

“…24 The effects of alkylating agents on DNA can be repaired by MGMT, leading to alkylating agent resistance. DNA MMR pathway is critical for mediating the cytotoxic effect of O 6 -methylguanine, which is programed to correct errors in DNA base pairing, and defects in this system cause resistance to temozolomide. 20 Another mechanism of resistance to alkylating agents is the BER pathway that can repair N 7 -methylguanine and N 3 -methyladenine DNA adducts.…”

mentioning

confidence: 99%

Overcoming tumor cell chemoresistance using nanoparticles: lysosomes are beneficial for (stearoyl) gemcitabine-incorporated solid lipid nanoparticles

Chen¹,

Zheng²,

Shi³

et al. 2018

IJN

Self Cite

View full text Add to dashboard Cite

Despite recent advances in targeted therapies and immunotherapies, chemotherapy using cytotoxic agents remains an indispensable modality in cancer treatment. Recently, there has been a growing emphasis in using nanomedicine in cancer chemotherapy, and several nanomedicines have already been used clinically to treat cancers. There is evidence that formulating small molecular cancer chemotherapeutic agents into nanomedicines significantly modifies their pharmacokinetics and often improves their efficacy. Importantly, cancer cells often develop resistance to chemotherapy, and formulating anticancer drugs into nanomedicines also helps overcome chemoresistance. In this review, we briefly describe the different classes of cancer chemotherapeutic agents, their mechanisms of action and resistance, and evidence of overcoming the resistance using nanomedicines. We then emphasize on gemcitabine and our experience in discovering the unique (stearoyl) gemcitabine solid lipid nanoparticles that are effective against tumor cells resistant to gemcitabine and elucidate the underlying mechanisms. It seems that lysosomes, which are an obstacle in the delivery of many drugs, are actually beneficial for our (stearoyl) gemcitabine solid lipid nanoparticles to overcome tumor cell resistance to gemcitabine.

show abstract

Nanomedicine: The Promise and Challenges in Cancer Chemotherapy

Cited by 24 publications

References 223 publications

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Improvement of pharmacokinetic and antitumor activity of layered double hydroxide nanoparticles by coating with PEGylated phospholipid membrane

Overcoming tumor cell chemoresistance using nanoparticles: lysosomes are beneficial for (stearoyl) gemcitabine-incorporated solid lipid nanoparticles

Contact Info

Product

Resources

About