Nanomechanical probing of microbubbles using the atomic force microscope

Sboros, Vassilis; Glynos, Emmanouil; Pye, S. D.; Moran, Carmel M.; Butler, Mairéad; Ross, James A.; McDicken, W.N.; Koutsos, Vasileios

doi:10.1016/j.ultras.2007.06.004

Cited by 43 publications

(39 citation statements)

References 28 publications

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“…These were chosen for their clinical relevance (Sboros et al 2007b). Data were collected in batches of three repeat measurements, at each set of incident parameters.…”

Section: Methodsmentioning

confidence: 99%

The Acoustic Scatter from Single biSphere Microbubbles

Thomas

Butler

Dermitzakis

et al. 2010

Ultrasound in Medicine & Biology

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“…These were chosen for their clinical relevance (Sboros et al 2007b). Data were collected in batches of three repeat measurements, at each set of incident parameters.…”

Section: Methodsmentioning

confidence: 99%

The Acoustic Scatter from Single biSphere Microbubbles

Thomas

Butler

Dermitzakis

et al. 2010

Ultrasound in Medicine & Biology

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“…These include micropipette aspiration (4), ultrasound scattering and attenuation measurements (5), fluorescence recovery after photobleaching (6), and atomic force microscopy (7). Estimates have also been successfully obtained through fitting theoretical models to acoustic and/or high-speed camera measurements of single microbubbles (8).…”

Section: Flim | Microviscositymentioning

confidence: 99%

Mapping microbubble viscosity using fluorescence lifetime imaging of molecular rotors

Hosny

Mohamedi

Rademeyer

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

138

114

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Encapsulated microbubbles are well established as highly effective contrast agents for ultrasound imaging. There remain, however, some significant challenges to fully realize the potential of microbubbles in advanced applications such as perfusion mapping, targeted drug delivery, and gene therapy. A key requirement is accurate characterization of the viscoelastic surface properties of the microbubbles, but methods for independent, nondestructive quantification and mapping of these properties are currently lacking. We present here a strategy for performing these measurements that uses a small fluorophore termed a "molecular rotor" embedded in the microbubble surface, whose fluorescence lifetime is directly related to the viscosity of its surroundings. We apply fluorescence lifetime imaging to show that shell viscosities vary widely across the population of the microbubbles and are influenced by the shell composition and the manufacturing process. We also demonstrate that heterogeneous viscosity distributions exist within individual microbubble shells even with a single surfactant component.

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“…Micron-sized, gas microbubbles encapsulated within a thin shell of lipid [1][2][3][4][5][6] or other surfactant based material [7][8][9][10][11] are generating increasing interest as ultrasound contrast agents (UCA's) [12][13][14] and potential drug delivery vehicles. [12][13][14][15][16][17][18] It is well documented that such microbubbles dissolve almost instantaneously in the absence of their surfactant coating 3 and that the coating not only affects the resistance to gas permeation, and hence microbubble lifetime, but also their mechanical properties.…”

Section: Introductionmentioning

confidence: 99%