Nanomaterials, Scaffolds, and Skin Tissue Regeneration

Leonida, Mihaela D.; Kumar, Ish

doi:10.1007/978-3-319-39168-7_12

Cited by 3 publications

(2 citation statements)

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“…Most significantly, it acts as the first protective and immunologic barrier in the body. Due to its high proliferating ability, skin can restore minor defects; nonetheless, severe full‐thickness wounds inhibit its self‐healing process to a great extent and place an enormous financial burden on healthcare societies and systems 1–11 …”

Section: Introductionmentioning

confidence: 99%

“…Due to its high proliferating ability, skin can restore minor defects; nonetheless, severe fullthickness wounds inhibit its self-healing process to a great extent and place an enormous financial burden on healthcare societies and systems. [1][2][3][4][5][6][7][8][9][10][11] In order to aid the regeneration of skin defects, skin grafts, including autografts, allografts (generally collected from cadavers), and xenografts, have been utilized as suitable treatments for skin damages. Briefly, skin grafting refers to the transplantation of the donor site's healthy skin to the wounded region.…”

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confidence: 99%

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Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

et al. 2023

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BackgroundEnhancing the efficiency of cell‐based skin tissue engineering (TE) approaches is possible via designing electrospun scaffolds possessing natural materials like amniotic membrane (AM) with wound healing characteristics. Concentrating on this aim, we fabricated innovative polycaprolactone (PCL)/AM scaffolds through the electrospinning process.MethodsThe manufactured structures were characterized by employing scanning electron microscope (SEM), attenuated total reflection‐Fourier transform infrared (ATR‐FTIR) spectroscopy, tensile testing, Bradford protein assay, etc. In addition, the mechanical properties of scaffolds were simulated by the multiscale modeling method.ResultsAs a result of conducting various tests, it was concluded that the uniformity and distribution of fibers decreased with an increase in the amniotic content. Furthermore, PCL‐AM scaffolds contained amniotic and PCL characteristic bands. In the case of protein release, greater content of AM led to the release of higher amounts of collagen. Tensile testing revealed that scaffolds' ultimate strength increased when the AM content augmented. The multiscale modeling demonstrated that the scaffold had elastoplastic behavior. In order to assess cellular attachment, viability, and differentiation, human adipose‐derived stem cells (ASCs) were seeded on the scaffolds. In this regard, SEM and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assays showed significant cellular proliferation and viability on the proposed scaffolds, and these analyses illustrated that higher cell survival and adhesion could be achieved when scaffolds possessed a larger amount of AM. After 21 days of cultivation, particular keratinocyte markers, such as keratin I and involucrin, were identified through utilizing immunofluorescence and real‐time polymerase chain reaction (PCR) tests. The markers' expressions were higher in the PCL‐AM scaffold with a ratio of 90:10 v v−1 compared with the PCL‐epidermal growth factor (EGF) structure. Moreover, the presence of AM in the scaffolds resulted in the keratinogenic differentiation of ASCs even without employing EGF. Consequently, this state‐of‐the‐art experiment suggests that the PCL‐AM scaffold can be a promising candidate in skin bioengineering.ConclusionThis study showed that mixing AM with PCL, a widely used polymer, in different concentrations can overcome PCL disadvantages such as high hydrophobicity and low cellular compatibility.

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Section: Introductionmentioning

confidence: 99%

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Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

et al. 2023

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Orthopedic Nanomaterials

Shokuhfar¹,

Firlar²,

Shirdar³

et al. 2017

Orthopedic Biomaterials

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Can Regenerative Medicine and Nanotechnology Combine to Heal wounds? the Search for the Ideal Wound Dressing

Zarrintaj

Moghaddam

Manouchehri

et al. 2017

Nanomedicine (Lond.)

174

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Skin is the outermost covering of the human body and at the same time the largest organ comprising 15% of body weight and 2 m surface area. Skin plays a key role as a barrier against the outer environment depending on its thickness, color and structure, which differ from one site to another. The four major types of problematic wounds include ulcers (diabetic, venous, pressure) and burn wounds. Developing novel dressings helps us to improve the wound healing process in difficult patients. Recent advances in regenerative medicine and nanotechnology are revolutionizing the field of wound healing. Antimicrobial activity, exogenous cell therapy, growth factor delivery, biodegradable and biocompatible matrix construction, all play a role in hi-tech dressing design. In the present review, we discuss how the principles of regenerative medicine and nanotechnology can be combined in innovative wound dressings.

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Nanomaterials, Scaffolds, and Skin Tissue Regeneration

Cited by 3 publications

References 37 publications

Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

Orthopedic Nanomaterials

Can Regenerative Medicine and Nanotechnology Combine to Heal wounds? the Search for the Ideal Wound Dressing

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