Nanomaterials modulate stem cell differentiation: biological interaction and underlying mechanisms

Wei, Min; Li, Song; Le, Weidong

doi:10.1186/s12951-017-0310-5

Cited by 90 publications

(76 citation statements)

References 146 publications

(186 reference statements)

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“…Tissue engineering is a multidisciplinary approach that has revolutionized regenerative medicine over the years. This research area has been enriched by the increasing progress of nanotechnology allowing the development of new methodologies to obtain different nanostructures, the understanding of their properties and the interactions between the nanomaterials and other types of constituents (Solanki, Kim, & Lee, ; Van Rijt & Habibovic, ; Wei, Li, & Le, ).…”

Section: Introductionmentioning

confidence: 99%

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Belluzo

Medina

Molinuevo

et al. 2020

J Biomedical Materials Res

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Section: Introductionmentioning

confidence: 99%

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Belluzo

Medina

Molinuevo

et al. 2020

J Biomedical Materials Res

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“…Given that a good surface hydrophilicity generally promotes cell adhesion and spreading 21,24 , in order to further characterize the substrate surface properties, we evaluated water wettability by performing water contact angle measurements ( Fig. 1f).…”

Section: Resultsmentioning

confidence: 99%

Neuronal contact guidance and YAP signaling on ultra-small nanogratings

Tonazzini

Masciullo

Savi

et al. 2020

Sci Rep

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contact interaction of neuronal cells with extracellular nanometric features can be exploited to investigate and modulate cellular responses. By exploiting nanogratings (nGs) with linewidth from 500 nm down to 100 nm, we here study neurite contact guidance along ultra-small directional topographies. the impact of nG lateral dimension on the neuronal morphotype, neurite alignment, focal adhesion (fA) development and YAp activation is investigated in nerve growth factor (nGf)differentiating PC12 cells and in primary hippocampal neurons, by confocal and live-cell total internal reflection fluorescence (TIRF) microscopy, and at molecular level. We demonstrate that loss of neurite guidance occurs in NGs with periodicity below 400 nm and correlates with a loss of FA lateral constriction and spatial organization. We found that YAP intracellular localization is modulated by the presence of nGs, but it is not sensitive to their periodicity. nocodazole, a drug that can increase cell contractility, is finally tested for rescuing neurite alignment showing mild ameliorative effects. Our results provide new indications for a rational design of biocompatible scaffolds for enhancing nerveregeneration processes.With its chemical and physical complexity, the extracellular environment can drive cell morphogenesis, migration and differentiation 1-4 , In particular, the contact interaction of cells with extracellular nanometric features has a primary role in regulating many physiological 5-12 and pathological processes 13 in vivo, and can be exploited to modulate cell responses in vitro [14][15][16][17] . Cells gather morphological information about the Extracellular matrix (ECM) through integrin-mediated adhesion clusters, called the focal adhesions (FAs), which act as topographical sensors. FAs can integrate multiple nanotopographical details into specific biomolecular instructions via the cytoskeletal signaling, which lastly regulates cell contractility, morphology and migration 9,18,19 , Artificial scaffolds with controlled micro/nano-topographies are definitively the apparatus of choice to better understand the response mechanisms of cells to external stimuli. They indeed allow to select and finely tune specific topographical aspects and selectively study their interaction with living system in vitro. The scientific literature reports many examples of nanostructures that can, for example, direct stem cell fate 20-22 , neuronal and glial adhesion 23-25 , differentiation 26 , polarization, and neurite orientation 14,18 , Our previous studies have demonstrated that plastic nanogratings (NGs) (i.e. alternating lines of sub-micron grooves and ridges, in the range between 500 and 2000 nm in linewidth) can promote neurite alignment and bipolarity of PC12 neuronal cells upon administration of nerve grow factor (NGF), simply by the contact guidance mechanism 27,28 , In these studies, adhesion on the ridges imposes a geometrical and directional constraint to FAs that results in neuronal polarization via the ROCK-mediated pathway 14,29-31 ...

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“…This long time period, for which the addition of NPs does not consist with toxic response, grants an extension provided either to their in vivo time of action or the in vitro induced differentiation towards the regeneration of damaged areas of the body. Several researchers have reported that using nanomaterials as 2D cell culture substrate could effectively promote the differentiation of stem cells into specific lineages, and the stiffness, surface chemistry, alignment and several other parameters of the cell culture substrate (matrix) may work together to influence the fate of stem cells [32]. Our findings provide strong evidence that the resulting nanocomposite PCL thin films not only passively interact with cells, but also serve as mechanical stimuli that trigger a series of biological alterations and modulate cell behaviors.…”

Section: Discussionmentioning

confidence: 99%

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

Koliakou

Gounari

Nerantzaki

et al. 2019

Tissue Eng Regen Med

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BACKGROUND: Lonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films. METHODS: For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO 2 ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells' genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton's Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay. RESULTS: The highest differentiation capacity of MOMCs was observed on PCL/SiO 2 ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation. CONCLUSION: To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO 2 ntbs or SrHAnrds into a polymeric matrix, for the first time. Graphical AbstractKeywords Monocyte-derived multipotential cells Á Poly(e-caprolactone) Á Silica nanotubes Á Strontium hydroxyapatite nanorods

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Nanomaterials modulate stem cell differentiation: biological interaction and underlying mechanisms

Cited by 90 publications

References 146 publications

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Neuronal contact guidance and YAP signaling on ultra-small nanogratings

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

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