Nanomaterials for T-cell cancer immunotherapy

Gong, Ningqiang; Sheppard, Neil C.; Billingsley, Margaret M.; June, Carl H.; Mitchell, Michael J.

doi:10.1038/s41565-020-00822-y

Cited by 219 publications

(151 citation statements)

References 147 publications

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“…On the other side, immunotherapies, including T-cells reactive toward tumor associated antigens and immune checkpoint blockers, are other formats of targeted therapies, which have recently shown to effectively prolong survival rate of patients suffering from advanced metastatic cancers [ 13 , 14 ]. Nevertheless, low neoantigen burden of most cancer cells and insufficient infiltration of immune cells to the tumor site, have mostly restricted effectiveness of immunotherapies in clinic.…”

Section: Introductionmentioning

confidence: 99%

Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

et al. 2021

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Development of engineered non-pathogenic bacteria, capable of expressing anti-cancer proteins under tumor-specific conditions, is an ideal approach for selectively eradicating proliferating cancer cells. Herein, using an engineered hypoxia responding nirB promoter, we developed an engineered Escherichia coli BW25133 strain capable of expressing cardiac peptides and GFP signaling protein under hypoxic condition for spatiotemporal targeting of mice mammary tumors. Following determination of the in vitro cytotoxicity profile of the engineered bacteria, selective accumulation of bacteria in tumor microenvironment was studied 48 h after tail vein injection of 108 cfu bacteria in animals. For in vivo evaluation of antitumoral activities, mice with establishment mammary tumors received 3 consecutive intravenous injections of transformed bacteria with 4-day intervals and alterations in expression of tumor growth, invasion and angiogenesis specific biomarkers (Ki-67, VEGFR, CD31and MMP9 respectively), as well as fold changes in concentration of proinflammatory cytokines were examined at the end of the 24-day study period. Intravenously injected bacteria could selectively accumulate in tumor site and temporally express GFP and cardiac peptides in response to hypoxia, enhancing survival rate of tumor bearing mice, suppressing tumor growth rate and expression of MMP-9, VEGFR2, CD31 and Ki67 biomarkers. Applied engineered bacteria could also significantly reduce concentrations of IL-1β, IL-6, GC-SF, IL-12 and TNF-α proinflammatory cytokines while increasing those of IL-10, IL-17A and INF-γ. Overall, administration of hypoxia-responding E. coli bacteria, carrying cardiac peptide expression construct could effectively suppress tumor growth, angiogenesis, invasion and metastasis and enhance overall survival of mice bearing mammary tumors.

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Section: Introductionmentioning

confidence: 99%

Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

et al. 2021

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“…Currently, the nanomedicine research has extended to targeted molecular therapy, involving not only small-molecule chemical drugs but also a variety of biologics, including nucleotide or protein drugs and cellular immunotherapy ( El-Zahaby et al, 2019 ; Li et al, 2020 ; Gong et al, 2021 ). These therapies are the important steps in overcoming some of the unique challenges of pancreatic cancers, which prevent conventional treatments from being effective.…”

Section: Discussionmentioning

confidence: 99%

Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

Cai

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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In this study, a chemically synthetic polymer, benzo[1,2-b:4,5-b′]difuran(BDF)-based donor–acceptor copolymer PBDFDTBO, was individually coated by amphiphilic poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-PCL) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol) (DSPE-PEG or PEG-DSPE), to form stably fluorescent nanoparticles in the near-infrared (NIR) window. The physicochemical properties of the synthesized nanoparticles were characterized and compared, including their size, surface charge, and morphology. In addition, in vitro studies were also performed using two pancreatic cancer cell lines, assessing the cell viability of the PBDFDTBO-included PEGylated nanoparticles formulations. Moreover, in vivo studies were also conducted, using subcutaneous murine cancer models to investigate the polymeric nanoparticles’ circulation time, tumor accumulation, and preferred organ biodistribution. The overall results demonstrated that even with the same PEGylated surface, the hydrophobic composition anchored on the encapsulated PBDFDTBO core strongly affected the biodistribution and tumor accumulation of the nanoparticles, to a degree possibly determined by the hydrophobic interactions between the hydrophobic segment of amphiphilic polymers (DSPE or PCL moiety) and the enwrapped PBDFDTBO. Both PEGylated nanoparticles were compared to obtain an optimized coating strategy for a desired biological feature in pancreatic cancer delivery.

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“…Nanomaterials are widely applied in various fields, such as pharmaceutical, automotive, and electronics industries and, most notably, in several areas within the chemical industry. An example of these is the drilling industry [ 92 , 93 ].…”

Section: Effects Of Nanomaterials In Drilling Fluidsmentioning

confidence: 99%

Utilization of Eco-Friendly Waste Generated Nanomaterials in Water-Based Drilling Fluids; State of the Art Review

et al. 2021

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An important aspect of hydrocarbon drilling is the usage of drilling fluids, which remove drill cuttings and stabilize the wellbore to provide better filtration. To stabilize these properties, several additives are used in drilling fluids that provide satisfactory rheological and filtration properties. However, commonly used additives are environmentally hazardous; when drilling fluids are disposed after drilling operations, they are discarded with the drill cuttings and additives into water sources and causes unwanted pollution. Therefore, these additives should be substituted with additives that are environmental friendly and provide superior performance. In this regard, biodegradable additives are required for future research. This review investigates the role of various bio-wastes as potential additives to be used in water-based drilling fluids. Furthermore, utilization of these waste-derived nanomaterials is summarized for rheology and lubricity tests. Finally, sufficient rheological and filtration examinations were carried out on water-based drilling fluids to evaluate the effect of wastes as additives on the performance of drilling fluids.

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Nanomaterials for T-cell cancer immunotherapy

Cited by 219 publications

References 147 publications

Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

Utilization of Eco-Friendly Waste Generated Nanomaterials in Water-Based Drilling Fluids; State of the Art Review

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