Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Samadi, Mitra; Majidzadeh‐A, Keivan; Salehi, Mina; Jalili, Neda; Noorinejad, Zeinab; Mosayebzadeh, Marjan; Muhammadnejad, Ahad; Khatibi, Azadeh Sharif; Moradi‐Kalbolandi, Shima; Farahmand, Leila

doi:10.1186/s13036-021-00269-2

Cited by 10 publications

(3 citation statements)

References 67 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This, together with lower ATP requirements (as explained above), may explain the greater formation of biomass and FbFP in strain PR1 under oxygen limitation. The performance of PR1 strain in oxygen‐limited regimes makes it an attractive host for the production of vaccines and antimicrobial peptides under microaerobic conditions, which can be preferred over aerobic regimes, to enhance the product activity (Abdolmohammadi Khiav & Zahmatkesh, 2021; Christmann et al, 2023; Samadi et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Recombinant protein expression in proteome‐reduced cells under aerobic and oxygen‐limited regimes

Lara,

Utrilla,

Martínez

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

Industrial cultures are hindered by the physiological complexity of the host and the limited mass transfer capacity of conventional bioreactors. In this study, a minimal cell approach was combined with genetic devices to overcome such issues. A flavin mononucleotide‐based fluorescent protein (FbFP) was expressed in a proteome‐reduced Escherichia coli (PR). When FbFP was expressed from a constitutive protein generator (CPG), the PR strain produced 47% and 35% more FbFP than its wild type (WT), in aerobic or oxygen‐limited regimes, respectively. Metabolic and expression models predicted more efficient biomass formation at higher fluxes to FbFP, in agreement with these results. A microaerobic protein generator (MPG) and a microaerobic transcriptional cascade (MTC) were designed to induce FbFP expression upon oxygen depletion. The FbFP fluorescence using the MTC in the PR strain was 9% higher than that of the WT bearing the CPG under oxygen limitation. To further improve the PR strain, the pyruvate dehydrogenase complex regulator gene was deleted, and the Vitreoscilla hemoglobin was expressed. Compared to oxygen‐limited cultures of the WT, the engineered strains increased the FbFP expression more than 50% using the MTC. Therefore, the designed expression systems can be a valuable alternative for industrial cultivations.

show abstract

Section: Resultsmentioning

confidence: 99%

Recombinant protein expression in proteome‐reduced cells under aerobic and oxygen‐limited regimes

Lara,

Utrilla,

Martínez

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…One of the problems with using antibodies is their large size; thus, researchers are trying to increase the tumor permeability by replacing antibodies with smaller peptides and aptamers [143,207]. Using specific peptides instead of antibodies could solve this limitation (large size) and enhance the tumor permeability.…”

Section: Epcam and Novel Therapymentioning

confidence: 99%

Anti-cancer therapeutic strategies based on HGF/MET, EpCAM, and tumor-stromal cross talk

et al. 2022

Self Cite

View full text Add to dashboard Cite

As an intelligent disease, tumors apply several pathways to evade the immune system. It can use alternative routes to bypass intracellular signaling pathways, such as nuclear factor-κB (NF-κB), Wnt, and mitogen-activated protein (MAP)/phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR). Therefore, these mechanisms lead to therapeutic resistance in cancer. Also, these pathways play important roles in the proliferation, survival, migration, and invasion of cells. In most cancers, these signaling pathways are overactivated, caused by mutation, overexpression, etc. Since numerous molecules share these signaling pathways, the identification of key molecules is crucial to achieve favorable consequences in cancer therapy. One of the key molecules is the mesenchymal-epithelial transition factor (MET; c-Met) and its ligand hepatocyte growth factor (HGF). Another molecule is the epithelial cell adhesion molecule (EpCAM), which its binding is hemophilic. Although both of them are involved in many physiologic processes (especially in embryonic stages), in some cancers, they are overexpressed on epithelial cells. Since they share intracellular pathways, targeting them simultaneously may inhibit substitute pathways that tumor uses to evade the immune system and resistant to therapeutic agents.

show abstract

“…Following publication of the original article [ 1 ], the authors reported missing information in the ‘Funding’ section.…”

mentioning

confidence: 99%

Correction: Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

et al. 2022

Self Cite

View full text Add to dashboard Cite

Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Cited by 10 publications

References 67 publications

Recombinant protein expression in proteome‐reduced cells under aerobic and oxygen‐limited regimes

Recombinant protein expression in proteome‐reduced cells under aerobic and oxygen‐limited regimes

Anti-cancer therapeutic strategies based on HGF/MET, EpCAM, and tumor-stromal cross talk

Correction: Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo

Contact Info

Product

Resources

About