Nanomaterials-based microbial sensor for direct electrochemical detection of Streptomyces Spp.

Hassan, Rabeay Y. A.; Hassan, H. N. A.; Abdel‐Aziz, Mohamed S.; Khaled, Elmorsy

doi:10.1016/j.snb.2014.07.059

Cited by 30 publications

(24 citation statements)

References 32 publications

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“…As a result, the incorporation of nanotubes into the electrode matrix enhanced the sensor performance through improving the conductivity and transduction of the chemical signal to electrical signal . However, the MWCNTs presented the highest performance towards the catalytic oxidation of POS.…”

Section: Resultsmentioning

confidence: 92%

“…The interesting properties of nano‐materials, such as nanoparticles, nanowires (NWs), carbon nanotubes (CNTs) and graphene make them very attractive as transducer for measuring the biological activity through facilitating electron transfer between the biomolecules and electrode surfaces . Particularly, the carbon nanotubes (CNTs) have attracted considerable attention due to their high electrical conductivity, strong adsorptive ability and good mechanical strength …”

Section: Introductionmentioning

confidence: 78%

“…The improvement is attributed to improving the electron transport of POS on electrode surface and facilitated the oxidation reaction owing to the catalytic properties of MWCNTs which increase the effective surface area of the electrode. This finding is correlated with our previous results which revealed the advantages of MWCNTs …”

Section: Resultsmentioning

confidence: 99%

“…Three electrode‐platforms of screen printed electrode (SPE): (DropSens: ref. DRP‐110 CNT), ceramic substrate: L 33×W 10×H 0.5 mm; working electrode (4 mm diameter) was made of carbon nanotubes, whereas the counter electrode was made of carbon, and the reference electrode and electric contacts were made of silver . The pH measurements were carried out using digital pH‐meter Mohr, cooling centrifugation was done on Centurion cooling centrifuge model (K2015R).…”

Section: Methodsmentioning

confidence: 94%

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A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

et al. 2016

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For the first time, the use of carbon nanotubes was exploited for the development of a sensitive electrochemical method for determination of the newly antifungal posaconazole (POS). The electrochemical activity of POS was investigated at the surface of multi‐walled nanotubes (MWNTs) modified electrode. The cyclic voltammograms showed a sharp oxidation peak at potential around 671 mV vs. Ag/AgCl. To reach the assay optimization, factors affecting the method sensitivity have been investigated, such as types of carbon nanotubes and its concentration in the electrode matrix, type of supporting electrolyte, pH, accumulation time and scan rate. A good linear relationship was obtained within the concentration range from 32–1280 ng/ml with the limit of detection and quantification of 11 ng/ml and 33 ng/ml, respectively. The proposed method was successfully applied for the determination of POS in its commercial dosage form, spiked human plasma samples, and dried blood spots. The in vivo results obtained were also used to study the pharmacokinetics of POS in human plasma. The results obtained were validated and found to be in accordance with those obtained by the reference methods.

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Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 94%

See 2 more Smart Citations

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

et al. 2016

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“…As shown in Figure , there are three independent bioelectrochemical approaches (direct detections, mediated detections, or labeling‐based detections) have been established , –, . Applying of biological recognition elements, such as cofactors, antibodies, microbes, organelles, tissues or high organisms cells .…”

Section: Methods Of Microbial Detectionmentioning

confidence: 99%

Bioelectrochemical Systems for Measuring Microbial Cellular Functions

Selim

Kamal²,

Ali

et al. 2017

Electroanalysis

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In bioelectrochemical systems (BESs), living microorganisms are capable of converting the chemical energy of degradable organic matters into bioelectricity. The electrical current outputs are dependent on the microbial cell viability and the biodegradation rates. Therefore, monitoring the current generative through the BES is promising for the microbial activity assessments. As compared to conventional microbiological methods, BESs are considered as non‐invasive techniques that offer rapid and sensitive detection of cellular functions (extra‐ and/or intracellular). Therefore, several progressions were made in the last 100 years in order to develop effective BESs. In this review, the involvements of materials sciences, microbiology, and electrochemistry in the effective designing and developments of BESs were intensively discussed. Due to the nanotechnology revolutions, manipulation of electrode materials led to the creation of different BES generations. Therefore, the impact of nanomaterials on the developments of the second and third generations of BESs is still the outlook of this promising research area.

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Direct Determination of Bacterial Cell Viability Using Carbon Nanotubes Modified Screen‐printed Electrodes

Hassan

Wollenberger

2019

Electroanalysis

Self Cite

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For the early detection of bacterial infection, there is a need for rapid, sensitive, and label‐free assays. Thus, in this study, nanostrucured microbial electrochemical platform is designed to monitor the viability and cell growth of S. aureus. Using multi‐walled carbon nanotube modified screen‐printed electrodes (MWCNTs/SPE), the cyclic voltammetric measurements showed only one irreversible oxidation peak at 600 mV vs Ag/AgCl that accounts for the viable and metabolically active bacterial cells. The assay was optimized and the secreted metabolites, in the extracellular matrix, were directly detected. The peak current showed a positive correlation with viable cell numbers ranging from OD600 nm of 0.1 to 1.1, indicating that the activity of live cells can be quantified. Consequently, responses of viable and non‐viable cells of S. aureus to the effects of antibiotic and respiratory chain inhibitors were determined. Thus, the proposed nanostructure‐based bacterial sensor provides a reasonable and reliable way for real‐time monitoring of live‐dead cell functions, and antibacterial profiling.

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Nanomaterials-based microbial sensor for direct electrochemical detection of Streptomyces Spp.

Cited by 30 publications

References 32 publications

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

Bioelectrochemical Systems for Measuring Microbial Cellular Functions

Direct Determination of Bacterial Cell Viability Using Carbon Nanotubes Modified Screen‐printed Electrodes

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