Nanomaterial-modulated cellular sodium extrusion and vacuolar sequestration for salt tolerance

Chen, Feiran; Zhu, Liqi; Tang, Junfeng; Wang, Chuanxi; Yue, Le; Schröder, Peter; Wang, Zhengyu

doi:10.1039/d2en00623e

Cited by 4 publications

(2 citation statements)

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“…Bacterial cell membrane is known as a barrier against HGT, inhibiting the transfer of eDNA into cells [ 45 , 46 ] ( Figure 4 a,b). Our previous work showed that CeO 2 NPs at low concentrations exhibited an efficient ROS scavenging capacity [ 47 ]. In System 1, although CeO 2 NPs at 10–50 mg L −1 increased the ROS level by 1.1-fold, ( Figure 4 c), the cell membrane permeability remained unchanged for all concentrations, and was confirmed by the expression pattern of genes related to membrane permeability and the SOS response ( Figure 4 d,e).…”

Section: Resultsmentioning

confidence: 99%

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

Wang

et al. 2023

Nanomaterials

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The direct uptake of extracellular DNA (eDNA) via transformation facilitates the dissemination of antibiotic resistance genes (ARGs) in the environment. CeO2 nanoparticles (NPs) have potential in the regulation of conjugation-dominated ARGs propagation, whereas their effects on ARGs transformation remain largely unknown. Here, CeO2 NPs at concentrations lower than 50 mg L−1 have been applied to regulate the transformation of plasmid-borne ARGs to competent Escherichia coli (E. coli) cells. Three types of exposure systems were established to optimize the regulation efficiency. Pre-incubation of competent E. coli cells with CeO2 NPs at 0.5 mg L−1 inhibited the transformation (35.4%) by reducing the ROS content (0.9-fold) and cell membrane permeability (0.9-fold), thereby down-regulating the expression of genes related to DNA uptake and processing (bhsA, ybaV, and nfsB, 0.7–0.8 folds). Importantly, CeO2 NPs exhibited an excellent binding capacity with the plasmids, decreasing the amounts of plasmids available for cellular uptake and down-regulating the gene expression of DNA uptake (bhsA, ybaV, and recJ, 0.6–0.7 folds). Altogether, pre-exposure of plasmids with CeO2 NPs (10 and 25 mg L−1) suppressed the transformation with an efficiency of 44.5–51.6%. This study provides a nano-strategy for controlling the transformation of ARGs, improving our understanding on the mechanisms of nanomaterial-mediated ARGs propagation.

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Section: Resultsmentioning

confidence: 99%

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

Wang

et al. 2023

Nanomaterials

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“…43 Additionally, previous studies have shown that NPs (e.g., carbon quantum dots, CeO 2 NPs) could promote Ca 2+ influx in plants owing to the small size effect of particles. 34,44 We thus speculated that nanoscale MSNs could also promote Ca 2+ influx. Moreover, phytohormones are critical regulators mediating plant resistance against herbivore infestation.…”

Section: Cs-msns Triggering Leaf Calcium and Phytohormone Signalingmentioning

confidence: 99%

Chitosan–silica nanocomposites induced resistance in faba bean plants against aphids (Acyrthosiphon pisum)

Ji¹,

Wang²,

Xue³

et al. 2023

Environ. Sci.: Nano

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Nano-CeO2 activates physical and chemical defenses of garlic (Allium sativum L.) for reducing antibiotic resistance genes in plant endosphere

Xu,

Tao,

et al. 2024

Ecotoxicology and Environmental Safety

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Nanomaterial-modulated cellular sodium extrusion and vacuolar sequestration for salt tolerance

Abstract: Nanomaterials can induce plant tolerance to abiotic environmental stresses, whereas the sensing mechanism and the resulting response at cellular level need further exploration. Bright Yellow 2 tobacco (Nicotiana tabacum L.)...

Cited by 4 publications

References 46 publications

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes

Chitosan–silica nanocomposites induced resistance in faba bean plants against aphids (Acyrthosiphon pisum)

Nano-CeO2 activates physical and chemical defenses of garlic (Allium sativum L.) for reducing antibiotic resistance genes in plant endosphere

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