Nanomagnetite-embedded PLGA Spheres for Multipurpose Medical Applications

Grumezescu, Valentina; Gherasim, Oana; Neguț, Irina; Banita, S.; Holban, Alina Maria; Florian, Paula; Icriverzi, Madalina; Socol, G.

doi:10.3390/ma12162521

Cited by 12 publications

(9 citation statements)

References 93 publications

(95 reference statements)

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“…Altogether, these results evidenced improved cellular attachment and spreading, demonstrating the highly biocompatible behavior of PLGA-based materials with respect to THP-1 differentiated human macrophages. These findings are in agreement with our previous study reporting the biocompatibility of Ti coatings based on IBUP-encapsulated PLGA microspheres and their ability to promote the adhesion and proliferation of macrophages [87].…”

Section: Effects Of Plga/ibup Coatings On Human Macrophagessupporting

confidence: 93%

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

et al. 2021

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To modulate the biofunctionality of implantable medical devices commonly used in clinical practice, their surface modification with bioactive polymeric coatings is an attractive and successful emerging strategy. Biodegradable coatings based on poly(lactic acid-co-glycolic acid), PLGA, represent versatile and safe candidates for surface modification of implantable biomaterials and devices, providing additional tunable ability for topical delivery of desired therapeutic agents. In the present study, Ibuprofen-loaded PLGA coatings (PLGA/IBUP) were obtained by using the dip-coating and drop-casting combined protocol. The composite materials demonstrated long-term drug release under biologically simulated dynamic conditions. Reversible swelling phenomena of polymeric coatings occurred in the first two weeks of testing, accompanied by the gradual matrix degradation and slow release of the therapeutic agent. Irreversible degradation of PLGA coatings occurred after one month, due to copolymer’s hydrolysis (evidenced by chemical and structural modifications). After 30 days of dynamic testing, the cumulative release of IBUP was ~250 µg/mL. Excellent cytocompatibility was revealed on human-derived macrophages, fibroblasts and keratinocytes. The results herein evidence the promising potential of PLGA/IBUP coatings to be used for surface modification of medical devices, such as metallic implants and wound dressings.

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Section: Effects Of Plga/ibup Coatings On Human Macrophagessupporting

confidence: 93%

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

et al. 2021

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“…Our obtained results support the antimicrobial efficiency of the AN extracts and show that the obtained coating can promote and ensure a prolonged activity of this extract for at least 72 h. Our results support previous findings regarding the efficiency of functionalized Fe 3 O 4 NPs against biofilm formation [ 68 ]. The impact of polymers, such as PLGA, in stabilizing and increasing the efficiency of the bioactive NPs (probably by increasing the load of the bioactive compounds or extract) is also supported by this study [ 69 , 70 ].…”

Section: Resultssupporting

confidence: 58%

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

Neguț

Grumezescu

et al. 2020

Molecules

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Plant extracts are highly valuable pharmaceutical complexes recognized for their biological properties, including antibacterial, antifungal, antiviral, antioxidant, anticancer, and anti-inflammatory properties. However, their use is limited by their low water solubility and physicochemical stability. In order to overcome these limitations, we aimed to develop nanostructured carriers as delivery systems for plant extracts; in particular, we selected the extract of Anthriscus sylvestris (AN) on the basis of its antimicrobial effect and antitumor activity. In this study, AN-extract-functionalized magnetite (Fe3O4@AN) nanoparticles (NPs) were prepared by the co-precipitation method. The purpose of this study was to synthesize and investigate the physicochemical and biological features of composite coatings based on Fe3O4@AN NPs obtained by matrix-assisted pulsed laser evaporation technique. In this respect, laser fluence and drop-casting studies on coatings were performed. The physical and chemical properties of laser-synthesized coatings were investigated by scanning electron microscopy, while Fourier transform infrared spectroscopy comparative analysis was used for determining the chemical structure and functional integrity. Relevant data regarding the presence of magnetic nanoparticles as the only crystalline phase and the size of nanoparticles were obtained by transmission electron microscopy. The in vitro toxicity assessment of the Fe3O4@AN showed significant cytotoxic activity against human adenocarcinoma HT-29 cells after prolonged exposure. Antimicrobial results demonstrated that Fe3O4@AN coatings inhibit microbial colonization and biofilm formation in clinically relevant bacteria species and yeasts. Such coatings are useful, natural, and multifunctional solutions for the development of tailored medical devices and surfaces.

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“…13,51,52 Different types of cells have been studied for this effect including macrophages, fibroblasts, osteoblasts, and HeLa, neuroblastoma and ovarian cancer cells. 44,51,[53][54][55][56][57][58][59] Since corona formation may affect MNP targeting and drug delivery, this phenomenon provides another rationale for laboratory modeling (such as our own), prior to resource-intense in vivo studies. The MBs studied here have been reported to rotate and display translational movement through serum and even blood, 10,11 fluid environments shown to generate a corona.…”

Section: Discussionmentioning

confidence: 99%

<p>Rotating Magnetic Nanoparticle Clusters as Microdevices for Drug Delivery</p>

Willis¹,

Pernal²,

Gaertner³

et al. 2020

IJN

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Background: Magnetic nanoparticles (MNPs) hold promise for enhancing delivery of therapeutic agents, either through direct binding or by functioning as miniature propellers. Fluid-filled conduits and reservoirs within the body offer avenues for MNP-enhanced drug delivery. MNP clusters can be rotated and moved across surfaces at clinically relevant distances in response to a rotating magnet. Limited data are available regarding issues affecting MNP delivery by this mechanism, such as adhesion to a cellular wall. Research reported here was initiated to better understand the fundamental principles important for successful implementation of rotational magnetic drug targeting (rMDT). Methods: Translational movements of four different iron oxide MNPs were tested, in response to rotation (3 Hz) of a neodymium-boron-iron permanent magnet. MNP clusters moved along biomimetic channels of a custom-made acrylic tray, by surface walking. The effects of different distances and cellular coatings on MNP velocity were analyzed using videography. Dyes (as drug surrogates) and the drug etoposide were transported by rotating MNPs along channels over a 10 cm distance. Results: MNP translational velocities could be predicted from magnetic separation times. Changes in distance or orientation from the magnet produced alterations in MNP velocities. Mean velocities of the fastest MNPs over HeLa, U251, U87, and E297 cells were 0.24 ± 0.02, 0.26 ± 0.02, 0.28 ± 0.01, and 0.18 ± 0.03 cm/sec, respectively. U138 cells showed marked MNP adherence and an 87.1% velocity reduction at 5.5 cm along the channel. Dye delivery helped visualize the effects of MNPs as microdevices for drug delivery. Dye delivery by MNP clusters was 21.7 times faster than by diffusion. MNPs successfully accelerated etoposide delivery, with retention of chemotherapeutic effect. Conclusion: The in vitro system described here facilitates side-by-side comparisons of drug delivery by rotating MNP clusters, on a human scale. Such microdevices have the potential for augmenting drug delivery in a variety of clinical settings, as proposed.

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Nanomagnetite-embedded PLGA Spheres for Multipurpose Medical Applications

Cited by 12 publications

References 93 publications

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

Nanostructured Thin Coatings Containing Anthriscus sylvestris Extract with Dual Bioactivity

<p>Rotating Magnetic Nanoparticle Clusters as Microdevices for Drug Delivery</p>

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