Nanomagnetic Competition Assay for Low-Abundance Protein Biomarker Quantification in Unprocessed Human Sera

Li, Yuanpeng; Balasubramanian, S.; Jing, Ying; Yao, Xiling; Hugger, Marie A.; Wang, Jian Ping; Xing, Chengguo

doi:10.1021/ja910406a

Cited by 77 publications

(49 citation statements)

References 24 publications

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“…Magnetoresistance ratio (MR) ratio for the full-film specular GMR sensor, measured by the field strength along the hard axis (transverse), is about 16% with a maximum sensitivity of 1.6% Oe-1 at 10 Oe field. Very slight hysteresis is shown in the loop for full film specular GMR [37,38,40,41]. The optical image of patterned specular GMR bioarrary is shown at Figure 4a.…”

Section: Resultsmentioning

confidence: 98%

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An Early Cancer Diagnosis Platform based on Micro-magnetic Sensor Array Demonstrates Ultra-high Sensitivity

Yang¹,

Li²

2016

J Nanomed Nanotechnol

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In this paper a highly sensitive detection methodology for obtaining the concentration of protein tumor markers via the selective incorporation of super paramagnetic iron oxide nanoparticles (MNP) onto an antigen is reported. The tumor marker concentration is measured with a nano-oxide layer inserted giant magneto-resistive (GMR) sensor that determines the attenuation of an external magnetic field that is induced by the MNP. The 15 nm MNPs, used herein, exhibit a super paramagnetic behavior with saturation magnetization of 36 emu/g. The output voltage signal of the Specular GMR sensor is 500 µV/Oe. The patterned GMR multiple-bio-sensor array, has demonstrated real-time measurements of carcinoembryonic antigen (CEA) protein concentrations down to femtomole level (10-15 mole) in a variety of clinically relevant media with a linear dynamic range of over five orders of magnitude. The sensitivity of the GMR bio-array platform is 1000 times higher than conventional enzyme-linked immunosorbent assay (ELISA) technique. The arrays of magnetoresistive sensors offer great promise in appli cations for early cancer diagnosis.

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Section: Resultsmentioning

confidence: 98%

“…For CEA sensing, it is ideal to have the particle labels either 20 nm or smaller in diameter [36]. However, the magnitude of the detectable magnetic field decrease with particle size, thus, a more sensitive sensors and measurement systems is required [37].…”

Section: Introductionmentioning

confidence: 99%

An Early Cancer Diagnosis Platform based on Micro-magnetic Sensor Array Demonstrates Ultra-high Sensitivity

Yang¹,

Li²

2016

J Nanomed Nanotechnol

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“…However, most reports of GMR biosensor research focused on theory, proof of concept [7,9,13,[20][21], or detection of spiked serum samples [17]. In this work, we demonstrate the accurate quantification of IL-6 with great specificity, in unprocessed human sera, employing our competition-based GMR biosensing system within 5 minutes [16]. In this work, we demonstrate the accurate quantification of IL-6 with great specificity, in unprocessed human sera, employing our competition-based GMR biosensing system within 5 minutes [16].…”

Section: Introductionmentioning

confidence: 93%

Biomarker Quantification in Unprocessed Human Sera by Using A New Nanomagnetic Protein Assay

Jing

Balasubramanian

et al. 2010

AIP Conference Proceedings

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Large hysteretic magnetoresistance in high-mobility semiconductor quantum wires bridged by single-domain nanomagnets Appl. Phys. Lett. 91, 022105 (2007); 10.1063/1.2756270Resonant microwave power absorption and relaxation of the energy levels of the molecular nanomagnet Fe 8 using superconducting quantum interference device-based magnetometry Appl. Phys. Lett.Abstract. Early recognition and prevention of chronic disease, such as lung cancer, require a fast, accurate detection and longitudinal monitoring on potential biomarkers, which could identify the molecule change in the initial stage of the chronic disease. Here we report the realization of specific and accurate quantification of a low-abundance serum protein in unprocessed human sera, using our novel giant magnetoresistive (GMR) biosensing system with uniform highmagnetic-moment nanoparticles and a competition based detection scheme. Only one antibody is needed for such detection scheme. The quantification of interleukin-6 (IL-6, a low-abundance protein and a potential cancer biomarker), as low as 125 fM IL-6 proteins, directly in 4 μL of unprocessed human sera was demonstrated within 5 minutes by such system. The results nicely differentiate normal individuals and lung cancer patients. This platform has great potential to facilitate the identification and validation of disease biomarkers.

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“…The authors developed a sandwich assay system for interleukin-6 and showed a linear dependence on concentration and limit of detection of about 2 ϫ 10 6 molecules. In further work, the same group demonstrated the use of a competition format to detect interleukin-6 down to concentrations of 50 fmol/L in human serum that had not been subjected to dialysis (66 ). Given the large-scale production of GMR devices for computer drives, it should be possible to manufacture these sensors on a large scale and at a low cost.…”

Section: Giant Magnetoresistive Sensor Technologymentioning

confidence: 99%

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

Gordon

Michel

2012

Clinical Chemistry

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BACKGROUND In the search for more powerful tools for diagnoses of endemic diseases in resource-limited settings, we have been analyzing technologies with potential applicability. Increasingly, the process focuses on readily accessible bodily fluids combined with increasingly powerful multiplex capabilities to unambiguously diagnose a condition without resorting to reliance on a sophisticated reference laboratory. Although these technological advances may well have important implications for the sensitive and specific detection of disease, to date their clinical utility has not been demonstrated, especially in resource-limited settings. Furthermore, many emerging technological developments are in fields of physics or engineering, which are not readily available to or intelligible to clinicians or clinical laboratory scientists. CONTENT This review provides a look at technology trends that could have applicability to high-sensitivity multiplexed immunoassays in resource-limited settings. Various technologies are explained and assessed according to potential for reaching relevant limits of cost, sensitivity, and multiplex capability. Frequently, such work is reported in technical journals not normally read by clinical scientists, and the authors make enthusiastic claims for the potential of their technology while ignoring potential pitfalls. Thus it is important to draw attention to technical hurdles that authors may not be publicizing. SUMMARY Immunochromatographic assays, optical methods including those involving waveguides, electrochemical methods, magnetorestrictive methods, and field-effect transistor methods based on nanotubes, nanowires, and nanoribbons reveal possibilities as next-generation technologies.

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Nanomagnetic Competition Assay for Low-Abundance Protein Biomarker Quantification in Unprocessed Human Sera

Cited by 77 publications

References 24 publications

An Early Cancer Diagnosis Platform based on Micro-magnetic Sensor Array Demonstrates Ultra-high Sensitivity

An Early Cancer Diagnosis Platform based on Micro-magnetic Sensor Array Demonstrates Ultra-high Sensitivity

Biomarker Quantification in Unprocessed Human Sera by Using A New Nanomagnetic Protein Assay

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

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