Nanolithography and its alternate techniques

Bhagoria, Palak; Sebastian, Elvin Mathew; Jain, Suyash Kumar; Purohit, Jahanvi; Purohit, Rajesh

doi:10.1016/j.matpr.2020.02.633

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…In vitro, numerous reports have demonstrated that topographical, physical, or biochemical features affect stem cell behavior, including adhesion, proliferation, and changes in cytoskeleton conformation. , Currently, researchers have developed many techniques for creating micro- or nanotopography. Common techniques include photolithography, electron beam lithography, dip pen nanolithography, and so on. ,− Very few publications have been found in the literature regarding the use of the spray technique for the preparation of micropatterned surfaces. , This should change considering the growing interest in synthesizing disordered micro-, nanopatterned surfaces: indeed, a novel concept was introduced by Dalby et al, who utilized electron beam lithography (EBL) to create nanotopographies with varying degrees of symmetry and disorder on the PMMA surface. They subsequently investigated how osteoprogenitor and mesenchymal stem cells responded to these nanoscale features .…”

Section: Discussionmentioning

confidence: 99%

Manipulating Stem Cell Fate with Disordered Bioactive Cues on Surfaces: The Role of Bioactive Ligand Selection

Zhang,

Remy,

Leste-Lasserre

et al. 2024

ACS Appl. Mater. Interfaces

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The development of 2D or 3D bioactive platforms for rapidly isolating pure populations of cells from adult stem cells holds promise for advancing the understanding of cellular mechanisms, drug testing, and tissue engineering. Over the years, methods have emerged to synthesize bioactive micro- and nanostructured 2D materials capable of directing stem cell fate. We introduce a novel method for randomly micro- or nanopatterning any protein/peptide onto both 2D and 3D scaffolds via spray technology. Our goal is to investigate the impact of arranging bioactive micropatterns (ordered vs disordered) on surfaces to guide human mesenchymal stem cell (hMSC) differentiation. The spray technology efficiently coats materials with controlled, cost-effective bioactive micropatterns in various sizes and shapes. BMP-2 mimetic peptides were covalently grafted, individually or in combination with RGD peptides, onto activated polyethylene terephthalate (PET) surfaces through a spraying process, incorporating nano/microscale parameters like size, shape, and composition. The study explores different peptide distributions on surfaces and various peptide combinations. Four surfaces were homogeneously functionalized with these peptides (M1 to M4 with various densities of peptides), and six surfaces with disordered micro- and nanopatterns of peptides (S0 to S5 with different sizes of peptide patterns) were synthesized. Fluorescence microscopy assessed peptide distribution, followed by hMSC culture for 2 weeks, and evaluated osteogenic differentiation via immunocytochemistry and RT-qPCR for osteoblast and osteocyte markers. Cells on uniformly peptide-functionalized surfaces exhibited cuboidal forms, while those on surfaces with disordered patterns tended toward columnar or cuboidal shapes. Surfaces S4 and S5 showed dendrite-like formations resembling an osteocyte morphology. S5 showed significant overexpression of osteoblast (OPN) and osteocyte markers (E11, DMP1, and SOST) compared to control surfaces and other micropatterned surfaces. Notably, despite sharing an equivalent quantity of peptides with a homogeneous functionalized surface, S5 displayed a distinct distribution of peptides, resulting in enhanced osteogenic differentiation of hMSCs.

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Section: Discussionmentioning

confidence: 99%

Manipulating Stem Cell Fate with Disordered Bioactive Cues on Surfaces: The Role of Bioactive Ligand Selection

Zhang,

Remy,

Leste-Lasserre

et al. 2024

ACS Appl. Mater. Interfaces

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“…Although nanolithography was a very niche branch before, it has developed in areas such as maskless patterning, reduced equipment usage, reduced power consumption, reduced skilled workers, and reduced experimental settings, etc. The nanolithography becomes the first choice for the IC manufacturing industry and nanoelectromechanical systems (NEMS) [ 105 ].…”

Section: Fabrication Methodsmentioning

confidence: 99%

Zero→Two-Dimensional Metal Nanostructures: An Overview on Methods of Preparation, Characterization, Properties, and Applications

et al. 2021

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Metal nanostructured materials, with many excellent and unique physical and mechanical properties compared to macroscopic bulk materials, have been widely used in the fields of electronics, bioimaging, sensing, photonics, biomimetic biology, information, and energy storage. It is worthy of noting that most of these applications require the use of nanostructured metals with specific controlled properties, which are significantly dependent on a series of physical parameters of its characteristic size, geometry, composition, and structure. Therefore, research on low-cost preparation of metal nanostructures and controlling of their characteristic sizes and geometric shapes are the keys to their development in different application fields. The preparation methods, physical and chemical properties, and application progress of metallic nanostructures are reviewed, and the methods for characterizing metal nanostructures are summarized. Finally, the future development of metallic nanostructure materials is explored.

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“…Nano-patterns do not require optical apparatus [36] Low throughput for batch manufacturing [37,38] Deposited material is controlled by hydrophobicity of the surface [36] Probe tip may be subjected to wear resulting in poor reproducibility [36] AFM tip can be changed to generate a random pattern [36] Ambient conditions need to be constant as humidity affects printing ink ensuing in deformed pattern. [36] In situ imaging capability [33] Hollow AFM tip can only permit certain compounds to pass. [36] DPN is effective in applications such as molecular electronics, material assembly and biological recognition [39].…”

Section: Advantages Disadvantagesmentioning

confidence: 99%

“…[36] In situ imaging capability [33] Hollow AFM tip can only permit certain compounds to pass. [36] DPN is effective in applications such as molecular electronics, material assembly and biological recognition [39]. Li et al utilized DPN technology to design versatile sensing patterns with multiple compositions and structures for biosensing.…”

Section: Advantages Disadvantagesmentioning

confidence: 99%

High Precision 3D Printing for Micro to Nano Scale Biomedical and Electronic Devices

et al. 2022

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Three dimensional printing (3DP), or additive manufacturing, is an exponentially growing process in the fabrication of various technologies with applications in sectors such as electronics, biomedical, pharmaceutical and tissue engineering. Micro and nano scale printing is encouraging the innovation of the aforementioned sectors, due to the ability to control design, material and chemical properties at a highly precise level, which is advantageous in creating a high surface area to volume ratio and altering the overall products’ mechanical and physical properties. In this review, micro/-nano printing technology,mainly related to lithography, inkjet and electrohydrodynamic (EHD) printing and their biomedical and electronic applications will be discussed. The current limitations to micro/-nano printing methods will be examined, covering the difficulty in achieving controlled structures at the miniscule micro and nano scale required for specific applications.

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Nanolithography and its alternate techniques

Cited by 7 publications

References 12 publications

Manipulating Stem Cell Fate with Disordered Bioactive Cues on Surfaces: The Role of Bioactive Ligand Selection

Manipulating Stem Cell Fate with Disordered Bioactive Cues on Surfaces: The Role of Bioactive Ligand Selection

Zero→Two-Dimensional Metal Nanostructures: An Overview on Methods of Preparation, Characterization, Properties, and Applications

High Precision 3D Printing for Micro to Nano Scale Biomedical and Electronic Devices

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