Nanoliposome-Loaded Phenolics from Nasturtium officinale Improves Health Parameters in a Colorectal Cancer Mouse Model

Abstract

Nasturtium officinale contains high amounts of phytochemical compounds that work against oxidative damages leading to improved health conditions in animals as well as humans. The study was performed to investigate the health benefits of nonencapsulated and nanoliposome-encapsulated phenolic rich fractions obtained from Nasturtium officinale on mice induced colorectal cancer. The experiment focused on encapsulation efficiency in improving the effectiveness of plant bioactive compounds. Phenolic rich fractions (PRF) were successfully loaded in the nanoliposome structure, a nanometer in size, of spherical shape and with homogeneous dispersion. Induction of colorectal cancer in mice impaired weight gain and feed intake, liver function and structural characteristics of ileum, while the dietary administration of nanoliposome-encapsulated PRF regulated the expression of Caspase 3, Bax, Bcl2, iNOS and SOD genes in the tumor tissue. The addition of nonencapsulated PRF and nanoliposome encapsulated PRF at the concentration of 100 mg TPC/kg BW/day improved the genes expression, although the nanoliposome-encapsulated PRF revealed better health outcomes compared to nonencapsulated PRF. Furthermore, both PRF improved intestinal morphology when the mice were challenged with colorectal cancer. The higher health promoting activity of nanoliposome-encapsulated PRF could be associated with its enhanced intestinal absorption, bioavailability, bioaccessibility and bioactivity. Consequently, the nanoliposome-encapsulated PRF could be considered as a promising anticancer agent against colorectal cancer.

Evaluation of dietary curcumin nanospheres as phytobiotics on growth performance, serum biochemistry, nutritional composition, meat quality, gastrointestinal health, and fecal condition of finishing pigs

“…In addition, Bao et al ( 29 ) did not find any significant effect on the dietary supplementation of nanocurcumin on overall performance and feed utilization in juvenile largemouth bass fish. In agreement with the present study, Taghavinia et al ( 25 ) found nanoliposome-loaded phenolics from Nasturtium officinale improved the average daily weight gain in mice. In relation to the curcumin nanobiotechnology in the present study, other researchers have proposed that nanoformulation of bioactive compounds such as microencapsulated organic acids, zinc oxide nanoparticles and coated sodium butyrate can be beneficial for enhancing growth and immunity in pigs ( 30 – 32 ).…”

“…Recently, researchers have emphasized the use of phytogenic compounds in the form of nanocapsules because most phytogenic materials are poorly bioavailable in biological systems. For this, incorporation of phytogenic compounds in nano encapsulation form with liposomal nano carrier showed most effective drug delivery systems in murine as a monogastric animal model for biomedical applications ( 22 – 25 ). Furthermore, it has been reported that liposomal nano carrier with its spherical form can easily entrap the lipophilic and hydrophobic curcumin, and protect itself from external stress mediated by oxygen, pH and enzymatic degradation during digestion and absorption in gastrointestinal tract ( 26 ).…”

Nanoliposome‐loaded phenolics from Salvia leriifolia Benth and its anticancer effects against induced colorectal cancer in mice

“…Afterward, a suspension of HT‐29 cells was obtained by trypsinization of confluent HT‐29 cells. To establish the HT‐29 carcinoma, 1 × 10 5 cells were injected into the right thigh of each mouse 20 . When the tumors became palpable (reaching a size of 18 mm 3 ), it was determined that the inoculation was successful.…”

“…After 28 days, the mice were euthanized with pentobarbital‐HCl (50 mg/kg, i.p.) and blood, liver, and tumor tissues were collected for cellular and molecular investigation 20 . All procedures involving the handling of animals in this study were conducted according to the regulations and guidelines established by the Islamic Azad University of Mashhad, Iran (IR.IAU.MSHD.REC.1399.018.…”

“…To establish the HT-29 carcinoma, 1 × 10 5 cells were injected into the right thigh of each mouse. 20 When the tumors became palpable (reaching a size of 18 mm 3 ), it was determined that the inoculation was successful. Here are the experimental treatments:…”