Nanohydroxyapatite as a Biomaterial for Peripheral Nerve Regeneration after Mechanical Damage—In Vitro Study

Wiatrak, Benita; Sobierajska, Paulina; Szandruk-Bender, Marta; Jawień, Paulina; Janeczek, Maciej; Pistor, Patrycja; Szeląg, Adam; Wiglusz, Rafał J.

doi:10.3390/ijms22094454

Cited by 15 publications

(4 citation statements)

References 44 publications

(69 reference statements)

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“…Benita Wiatrak et al [64] showed that nanohydroxyapatite molecules can also induce nerve regeneration. There is an interaction between the time of nerve inflammation and an increase in the activity of mitochondria in neurons affected by hydroxyapatite nanoparticles.…”

Section: Hydroxyapatite (Hap)mentioning

confidence: 99%

Nanomaterials Application in Endodontics

et al. 2021

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In recent years, nanomaterials have become increasingly present in medicine, especially in dentistry. Their characteristics are proving to be very useful in clinical cases. Due to the intense research in the field of biomaterials and nanotechnology, the efficacy and possibilities of dental procedures have immensely expanded over the years. The nano size of materials allows them to exhibit properties not present in their larger-in-scale counterparts. The medical procedures in endodontics are time-consuming and mostly require several visits to be able to achieve the proper result. In this field of dentistry, there are still major issues about the removal of the mostly bacterial infection from the dental root canals. It has been confirmed that nanoparticles are much more efficient than traditional materials and appear to have superior properties when it comes to surface chemistry and bonding. Their unique antibacterial properties are also promising features in every medical procedure, especially in endodontics. High versatility of use of nanomaterials makes them a powerful tool in dental clinics, in a plethora of endodontic procedures, including pulp regeneration, drug delivery, root repair, disinfection, obturation and canal filling. This study focuses on summing up the current knowledge about the utility of nanomaterials in endodontics, their characteristics, advantages, disadvantages, and provides a number of reasons why research in this field should be continued.

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Section: Hydroxyapatite (Hap)mentioning

confidence: 99%

Nanomaterials Application in Endodontics

et al. 2021

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“…Taking advantage of the properties of conductive and biodegradable polymers, their scaffold was designed to promote directional axonal growth and migration of Schwann cells via the microstructure of the biodegradable polymer fibers [ 26 ]. In addition, a recent study by Wiatrak et al 2021, showed that nanocrystalline apatite doped and co-doped with Li + and Eu 3+ ions might be a very attractive biomaterial for the regeneration of nervous tissue, where europium ions influence neuronal features even more strongly than doping with lithium alone [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder

Thurzo

Gálfiová

Nováková

et al. 2022

IJMS

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This paper presents a proof-of-concept study on the biocolonization of 3D-printed hydroxyapatite scaffolds with mesenchymal stem cells (MSCs). Three-dimensional (3D) printed biomimetic bone structure made of calcium deficient hydroxyapatite (CDHA) intended as a future bone graft was made from newly developed composite material for FDM printing. The biopolymer polyvinyl alcohol serves in this material as a thermoplastic binder for 3D molding of the printed object with a passive function and is completely removed during sintering. The study presents the material, the process of fused deposition modeling (FDM) of CDHA scaffolds, and its post-processing at three temperatures (1200, 1300, and 1400 °C), as well it evaluates the cytotoxicity and biocompatibility of scaffolds with MTT and LDH release assays after 14 days. The study also includes a morphological evaluation of cellular colonization with scanning electron microscopy (SEM) in two different filament orientations (rectilinear and gyroid). The results of the MTT assay showed that the tested material was not toxic, and cells were preserved in both orientations, with most cells present on the material fired at 1300 °C. Results of the LDH release assay showed a slight increase in LDH leakage from all samples. Visual evaluation of SEM confirmed the ideal post-processing temperature of the 3D-printed FDM framework for samples fired at 1300 °C and 1400 °C, with a porosity of 0.3 mm between filaments. In conclusion, the presented fabrication and colonization of CDHA scaffolds have great potential to be used in the tissue engineering of bones.

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“…Taking advantage of the properties of conductive and biodegradable polymers, their scaffold was designed to promote directional axonal growth and migration of Schwann cells via the microstructure of the biodegradable polymer fibers [22]. Also, a recent study of Wiatrak et al 2021, showed that nanocrystalline apatite doped and co-doped with Li + and Eu 3+ ions may be a very attractive biomaterial for the regeneration of nervous tissue, where europium ions influence neuronal features even more strongly than doping with lithium alone [23].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as A Thermoplastic Binder

Thurzo¹,

Gálfiová²,

Nováková³

et al. 2022

Preprint

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This paper presents a proof-of-concept study on the biocolonization of 3D-printed hydroxyapatite scaffolds with mesenchymal stem cells (MSCs). Three-dimensional (3D) printed biomimetic bone structure made of Calcium Deficient HydroxyApatite (CDHA) intended as future bone graft was made from newly developed composite material for FDM printing. The biopolymer polyvinyl alcohol serves in this material as a thermoplastic binder for 3D molding of the printed object with a passive function and is completely removed during sintering. The study presents the material, the process of fused deposition modeling (FDM) of CDHA scaffolds and its post-processing at three temperatures (1200, 1300, 1400 °C), as well it evaluates the cytotoxicity and biocompatibility of scaffolds with MTT and LDH release assays after 14 days. The study also includes a morphological evaluation of the cellular colonization with scanning electron microscopy (SEM) in two different filament orientations (rectilinear and gyroid). The results of the MTT assay showed that the tested material was not toxic, and cells were preserved in both orientations, with most cells present on the material fired at 1300°C. Results of the LDH release assay showed a slight increase in LDH leakage from all samples. Visual evaluation of SEM confirmed the ideal post-processing temperature of the 3D-printed FDM framework for samples fired at 1300°C and 1400°C, with a porosity of 0.3 mm between filaments. In conclusion, the presented fabrication and colonization of CDHA scaffolds have great potential to be used in the tissue engineering of bones.

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Nanohydroxyapatite as a Biomaterial for Peripheral Nerve Regeneration after Mechanical Damage—In Vitro Study

Cited by 15 publications

References 44 publications

Nanomaterials Application in Endodontics

Nanomaterials Application in Endodontics

Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder

Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as A Thermoplastic Binder

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