Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder

Thurzo, Andrej; Gálfiová, Paulína; Nováková, Zuzana; Polák, Štefan; Varga, Ivan; Strunga, Martin; Surovková, Jana; Leško, Ľuboš; Hajdúchová, Zora; Feranc, Jozef; Janek, M.; Danišovič, Ľuboš

doi:10.3390/ijms232314870

Cited by 20 publications

(18 citation statements)

References 53 publications

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“…The MTT test (Proliferation Kit I, Roche, Basel, Switzerland) was applied to assess each NGC at 24 and 48 h [ 30 ]. NGCs were transferred to a new 96-well plate supplemented with 100 µL of Ham’s F12, and 10 µL of “MTT labeling reagent, 1×” (final concentration 0.5 mg/mL) was added to each NGC-containing well.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Gutiérrez,

González-Quijón,

Martínez-Rodríguez

et al. 2024

Polymers

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Background: The elaboration of biocompatible nerve guide conduits (NGCs) has been studied in recent years as a treatment for total nerve rupture lesions (axonotmesis). Different natural polymers have been used in these studies, including cellulose associated with soy protein. The purpose of this report was to describe manufacturing NGCs suitable for nerve regeneration using the method of dip coating and evaporation of solvent with cellulose acetate (CA) functionalized with soy protein acid hydrolysate (SPAH). Methods: The manufacturing method and bacterial control precautions for the CA/SPAH NGCs were described. The structure of the NGCs was analyzed under a scanning electron microscope (SEM); porosity was analyzed with a degassing method using a porosimeter. Schwann cell (SCL 4.1/F7) biocompatibility of cell-seeded nerve guide conduits was evaluated with the MTT assay. Results: The method employed allowed an easy elaboration and customization of NGCs, free of bacteria, with pores in the internal surface, and the uniform wall thickness allowed manipulation, which showed flexibility; additionally, the sample was suturable. The NGCs showed initial biocompatibility with Schwann cells, revealing cells adhered to the NGC structure after 5 days. Conclusions: The fabricated CA/SPAH NGCs showed adequate features to be used for peripheral nerve regeneration studies. Future reports are necessary to discuss the ideal concentration of CA and SPAH and the mechanical and physicochemical properties of this biomaterial.

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Section: Methodsmentioning

confidence: 99%

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Gutiérrez,

González-Quijón,

Martínez-Rodríguez

et al. 2024

Polymers

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“…They can modify responses of cells, increase their surface adhesion, differentiation, osteoblastic and odontoblastic activity, osteoconductivity, mineralization processes, antimicrobial effects, vascularization, and other mechanical and biological functions. These numerous effects determine the applications of biomaterials in regenerative dentistry [110][111][112][113]. In fact, biomaterials in the form of scaffolds have shown favorable properties in restorative dentistry, endodontics, implantology, and maxillofacial surgery [110,114,115].…”

Section: Materials For Scaffold Fabrication In Regenerative Dentistrymentioning

confidence: 99%

Evolving Strategies and Materials for Scaffold Development in Regenerative Dentistry

Gašparovič,

Jungová,

Tomášik

et al. 2024

Applied Sciences

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Regenerative dentistry has experienced remarkable advancement in recent years. The interdisciplinary discoveries in stem cell applications and scaffold design and fabrication, including novel techniques and biomaterials, have demonstrated immense potential in the field of tissue engineering and regenerative therapy. Scaffolds play a pivotal role in regenerative dentistry by facilitating tissue regeneration and restoring damaged or missing dental structures. These biocompatible and biomimetic structures serve as a temporary framework for cells to adhere, proliferate, and differentiate into functional tissues. This review provides a concise overview of the evolution of scaffold strategies in regenerative dentistry, along with a novel analysis (Bard v2.0 based on the Gemini neural network architecture) of the most commonly employed materials used for scaffold fabrication during the last 10 years. Additionally, it delves into bioprinting, stem cell colonization techniques and procedures, and outlines the prospects of regenerating a whole tooth in the future. Moreover, it discusses the optimal conditions for maximizing mesenchymal stem cell utilization and optimizing scaffold design and personalization through precise 3D bioprinting. This review highlights the recent advancements in scaffold development, particularly with the advent of 3D bioprinting technologies, and is based on a comprehensive literature search of the most influential recent publications in this field.

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“…For example, research has been conducted on the fabrication and in vitro characterization of novel 3D-printed hydroxyapatite scaffolds. Various proof-of-concept studies have been published on the biocolonization of 3D-printed hydroxyapatite scaffolds using mesenchymal stem cells [ 170 ].…”

Section: New Findings From Clinical and Basic Research On The Alveola...mentioning

confidence: 99%

Recent Clinical Treatment and Basic Research on the Alveolar Bone

Tsuchida

Nakayama

2023

Biomedicines

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The periodontal ligament is located between the bone (alveolar bone) and the cementum of the tooth, and it is connected by tough fibers called Sharpey’s fibers. To maintain healthy teeth, the foundation supporting the teeth must be healthy. Periodontal diseases, also known as tooth loss, cause the alveolar bone to dissolve. The alveolar bone, similar to the bones in other body parts, is repeatedly resorbed by osteoclasts and renewed by osteogenic cells. This means that an old bone is constantly being resorbed and replaced by a new bone. In periodontal diseases, the alveolar bone around the teeth is absorbed, and as the disease progresses, the alveolar bone shrinks gradually. In most cases, the resorbed alveolar bone does not return to its original form even after periodontal disease is cured. Gum covers the tooth surface so that it matches the shape of the resorbed alveolar bone, exposing more of the tooth surface than before, making the teeth look longer, leaving gaps between the teeth, and in some cases causing teeth to sting. Previously, the only treatment for periodontal diseases was to stop the disease from progressing further before the teeth fell out, and restoration to the original condition was almost impossible. However, a treatment method that can help in the regeneration of the supporting tissues of the teeth destroyed by periodontal diseases and the restoration of the teeth to their original healthy state as much as possible is introduced. Recently, with improvements in implant material properties, implant therapy has become an indispensable treatment method in dentistry and an important prosthetic option. Treatment methods and techniques, which are mainly based on experience, have gradually accumulated scientific evidence, and the number of indications for treatment has increased. The development of bone augmentation methods has contributed remarkably to the expansion of indications, and this has been made possible by various advances in materials science. The induced pluripotent stem cell (iPS) cell technology for regenerating periodontal tissues, including alveolar bone, is expected to be applied in the treatment of diseases, such as tooth loss and periodontitis. This review focuses on the alveolar bone and describes clinical practice, techniques, and the latest basic research.

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Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder

Cited by 20 publications

References 53 publications

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Evolving Strategies and Materials for Scaffold Development in Regenerative Dentistry

Recent Clinical Treatment and Basic Research on the Alveolar Bone

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