Nanogold-functionalized magnetic beads with redox activity for sensitive electrochemical immunoassay of thyroid-stimulating hormone

Zhang, Bing; Tang, Dianping; Liu, Bingqian; Cui, Yuling; Chen, Huafeng; Chen, Guonan

doi:10.1016/j.aca.2011.10.049

Cited by 41 publications

(16 citation statements)

References 29 publications

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“…The enormous signal enhancement associated with the use of nano labels provides the basis for ultrasensitive immunosensor and immunoassays with different measurement techniques [18].…”

Section: Nanoparticles As Signal Labels For Signal Amplificationmentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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To dissolve the bottleneck problem of life and biomedical science in detection of biomolecules with low abundance and acquisition of ultraweak biological signals, highly sensitive analytical methods coupling with the specificity of biological recognition events have been quickly developed by the exploring of signal amplification strategies. These strategies have extensively been introduced into the development of highly sensitive immunosensing methods by combining with highly specific immunological recognition. They can be divided into two groups. One group of strategies attempts to transfer the immunological recognition event into large number of reporter probes or signal probes for signal readout by employing nano/micro-materials as vehicles for multi-labeling and/or molecular biological amplification for increasing the abundance of the signal molecules. The other uses nanomaterials or enzyme mimics as catalytic tools/tags to obtain enhanced detection signal. This review focuses on the significant advances in design of signal amplification strategies for highly sensitive immunosensing.

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“…The enormous signal enhancement associated with the use of nano labels provides the basis for ultrasensitive immunosensor and immunoassays with different measurement techniques [18].…”

Section: Nanoparticles As Signal Labels For Signal Amplificationmentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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“…Zhang et al [126] designed a novel electrochemical immunosensor for sensitive determination of TSH utilizing nanogold-functionalized magnetic beads (GoldMag) on a gold nanoparticle-dispersed graphene nanosheets immunosensing platform. The authors prepared polyethyleneimine-functionalized magnetic beads by a wet chemical method onto which electroactive thionine molecules and gold nanoparticles were alternately immobilized using and opposite-charged adsorption technique and an in situ synthesis method, respectively.…”

Section: Biomarkers As Analytesmentioning

confidence: 99%

“…The conjugated signal tags increased with increasing TSH concentration which was also determined in human serum specimens. This electrochemical immunosensor had detection limit of 0.23 pg/mL and a linear range from 0.45 pg/mL to 0.91 ng/mL [126]. The results indicated that the GoldMag nanostructures, conjugated with HRP, enhanced the sensitivity of the electrochemical immunosensor.…”

Section: Biomarkers As Analytesmentioning

confidence: 99%

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

2014

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Nanotechnology has played a crucial role in the development of biosensors over the past decade. The development, testing, optimization, and validation of new biosensors has become a highly interdisciplinary effort involving experts in chemistry, biology, physics, engineering, and medicine. The sensitivity, the specificity and the reproducibility of biosensors have improved tremendously as a result of incorporating nanomaterials in their design. In general, nanomaterials-based electrochemical immunosensors amplify the sensitivity by facilitating greater loading of the larger sensing surface with biorecognition molecules as well as improving the electrochemical properties of the transducer. The most common types of nanomaterials and their properties will be described. In addition, the utilization of nanomaterials in immunosensors for biomarker detection will be discussed since these biosensors have enormous potential for a myriad of clinical uses. Electrochemical immunosensors provide a specific and simple analytical alternative as evidenced by their brief analysis times, inexpensive instrumentation, lower assay cost as well as good portability and amenability to miniaturization. The role nanomaterials play in biosensors, their ability to improve detection capabilities in low concentration analytes yielding clinically useful data and their impact on other biosensor performance properties will be discussed. Finally, the most common types of electroanalytical detection methods will be briefly touched upon.

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“…However, the high cost and complexity of this preparation strategy made it difficult for commercialization. In addition, some analogous bead-on-bead structures were also used in several researches [11][12] to develop labeled carriers and improve detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Improvement of immunoassay detection sensitivity by using well-defined raspberry-like magnetic microbeads as carriers

Wang

Zhang

et al. 2014

J. Shanghai Jiaotong Univ. (Sci.)

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Well-defined raspberry-like magnetic microbeads (RMMBs) as immunoassay solid carriers were prepared by chemical covalent binding between Fe3O4 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles (< 200 nm), which was an advangtage for high efficient magnetic separation. When compared to Fe3O4@SiO2 core-shell magnetic microbeads (CMMBs) with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank (LoB) and limit of detection (LoD) of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay (CLIA) were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.

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Nanogold-functionalized magnetic beads with redox activity for sensitive electrochemical immunoassay of thyroid-stimulating hormone

Cited by 41 publications

References 29 publications

Signal Amplification for Highly Sensitive Immunosensing

Signal Amplification for Highly Sensitive Immunosensing

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

Improvement of immunoassay detection sensitivity by using well-defined raspberry-like magnetic microbeads as carriers

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