Nanogold based lateral flow assay for the detection of Salmonella typhi in environmental water samples

Singh, Jyoti; Sharma, Shivesh; Nara, Seema

doi:10.1039/c5ay02271a

Cited by 30 publications

(16 citation statements)

References 23 publications

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“…The lateral flow assay (LFA) method is a simple and powerful tool that can detect a variety of analytes from blood proteins to mycotoxins and from viral pathogens to bacterial toxins [15,16,17,18,19,20,21,22,23,24]. Most widely used LFA-based biosensors depend on changes in colorimetric signals that originate from the aggregation of colloidal gold nanoparticles (AuNPs) [15].…”

Section: Introductionmentioning

confidence: 99%

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Kim

Hong

et al. 2016

Sensors

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In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

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Section: Introductionmentioning

confidence: 99%

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Kim

Hong

et al. 2016

Sensors

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“…The common preparing methods of goldnanoparticle include sodium citrate, sodium borohydride, ascorbic acid, microwave, photochemical and microorganism methods36373839404142 (Table S6). Sodium citrate method is simple, but the SERS activity is low.…”

Section: Resultsmentioning

confidence: 99%

SERS spectral study of HAuCl4-cysteine nanocatalytic reaction and its application for detection of heparin sodium with label-free VB4r molecular probe

Wang

Jiang

Qin

et al. 2017

Sci Rep

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In the presence of nanocatalyst, L-cysteine reduce HAuCl4 rapidly to form gold nanoparticles (AuNP), and a quick nanocatalytic preparation procedure was established for Au/AuNP sol with highly active surface enhanced Raman scattering (SERS) effect and good stability. The nanoreaction was also studied by absorption, resonance Rayleigh scattering (RRS), transmission electron microscopy (TEM) and energy spectra. In the selected conditions, the analyte heparin sodium (HS) could react with victoria blue 4 R (VB4r) to form associated complexes which have very weak SERS effect to make the SERS signals decrease. The SERS signals at 1617 cm−1 reduced linearly with HS concentration increasing. Upon addition of FeCl3, it hydrolyzed to form stable Fe(OH)3 sol platform that carried SERS active Au/AuNPs to enhance the sensitivity. Accordingly, we established a SERS quantitative analysis method in the sol substrate of Fe(OH)3-Au/AuNPs, with a linear range of 0.5–75 ng/mL HS and a detection limit of 0.2 ng/mL. HS in real samples was determined, with a relative standard deviation of 2.65–7.63% and a recovery of 99.3–101%.

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“…1 Commonly employed for home use as the home pregnancy test (to detect human chorionic gonadotropin), LFAs have been demonstrated to detect antibodies, 2 viruses, 3-5 bacteria, 6, 7 parasites, 8 nucleic acids, 9-11 proteins, 12-14 drugs, 15, 16 and toxins. 17, 18 In the commonly-employed sandwich lateral flow format, particulate reporters (traditionally gold nanoparticles) are functionalized with antibodies to the analyte of interest.…”

Section: Introductionmentioning

confidence: 99%

“…Sandwich LFAs require little or no sample preparation and generate a rapid and easily-read result, significant advantages for a point-of-use diagnostic in resource-limited settings. 5, 7, 19 In most current implementations, however, LFAs are semi-quantitative and are insufficiently sensitive to detect analytes at extremely low concentrations – for example, to detect most viruses at clinically-relevant concentrations. Indeed, a wide range of applications requiring point-of-use monitoring, including biopharmaceutical manufacturing, food processing, environmental monitoring, and biodefense, would benefit from new LFA formats with improved sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay

Kim

Poling-Skutvik

Trabuco

et al. 2017

Analyst

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Using microscopy and image analysis, we characterize binding of filamentous viral nanoparticles to a fibrous affinity matrix as models for reporter capture in a lateral flow assay (LFA). M13 bacteriophage (M13) displaying an in vivo-biotinylated peptide (AviTag) genetically fused to the M13 tail protein p3 are functionalized with fluorescent labels. We functionalize glass fiber LFA membranes with antibodies to M13, which primarily capture M13 on the major p8 coat proteins, or with avidin, which captures M13 at the biotin-functionalized tail, and compare orientational modes of reporter capture for the side- versus tip-binding recognition interactions. The number of captured M13 is greater for side-binding than for tip-binding, as expected from the number of recognition groups. Whereas two-thirds of side-bound M13 captured by an anti-M13 antibody bind immediately after colliding with the membrane, tip-bound M13 prominently exhibit three additional orientational modes that require M13 to reorient to enable binding. These results are consistent with the idea that the elongated M13 shape couples with the complex flow field in an open and disordered fibrous LFA membrane to enhance capture.

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Nanogold based lateral flow assay for the detection of Salmonella typhi in environmental water samples

Abstract: Background: Salmonella infection is one of the leading causes of water and food borne diseases and is the cause of approximately 3.5–18 million deaths per year worldwide.

Cited by 30 publications

References 23 publications

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

SERS spectral study of HAuCl4-cysteine nanocatalytic reaction and its application for detection of heparin sodium with label-free VB4r molecular probe

Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay

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