Nanogapped impedimetric immunosensor for the detection of 16 kDa heat shock protein against Mycobacterium tuberculosis

Gopinath, Subash C. B.; Perumal, Veeradasan; Kumaresan, R.; Lakshmipriya, Thangavel; Rajintraprasad, Haarindraprasad; Balakrishnan, Sharma Rao; Arshad, M. K. Md; Chen, Yeng; Kotani, Norito; Hashim, U.

doi:10.1007/s00604-016-1911-7

Cited by 50 publications

(13 citation statements)

References 32 publications

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“…Gold core defines the fundamental properties of a GNP, and the layer surrounding the core is formed by the electrostatic interaction of the molecules in the biological milieu. GNPs were used for various applications such as gene delivery, drug delivery, antitumor, antimicrobial, cancer therapy, antioxidant, and catalytic use [23,[37][38][39]. The present study aimed to demonstrate that the active components in A. paniculata are responsible for antimicrobial potential.…”

Section: Introductionmentioning

confidence: 93%

Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

Razalli¹,

Gopinath²,

Kasim³

et al. 2019

INNOCS Theranos Pharmco Sci

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Background: Herbal extracts have been traditionally used as antibacterial agents, and different strategies have been proposed to enhance their antimicrobial activities. This research aimed to assess the antimicrobial potential of Andrographis paniculata conjugated gold nanoparticle (GNP) compounds against Escherichia coli and Bacillus subtilis.Methods: Herbal extract was conjugated with GNP through electrostatic interaction, and characterization was carried out by Fourier-transmission infrared spectroscopy, screening electron microscopy, and transmission electron microscopy and supported by energy-dispersive X-ray spectroscopy. Antibacterial activity of the herbal extract and GNP conjugation was evaluated by disc diffusion assay.Results: Chemical and morphological characterizations of the GNP and herbal extract conjugate revealed intactness of the GNP. In disc diffusion assay, the inhibition zone formed by these compounds was measured for both microorganisms, and they were in the range of 0.6-0.9 cm for the herbal extract. When the herbal extract conjugated with GNP, the zone was between 0.8 and 1.1 cm.Conclusion: This study demonstrated the potentiality of GNP to carry the antibacterial compounds from the herbal extract.

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Section: Introductionmentioning

confidence: 93%

Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

Razalli¹,

Gopinath²,

Kasim³

et al. 2019

INNOCS Theranos Pharmco Sci

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“…with a detection limit as low as 100 fM (Gopinath et al, 2016). Aptamers are artificially synthesized short singlestranded DNA or RNA, which can develop high-affinity molecules to specifically recognize the desired target.…”

Section: Components Identificationmentioning

confidence: 99%

Advances in airborne microorganisms detection using biosensors: A critical review

Wang

et al. 2021

Front. Environ. Sci. Eng.

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Humanity has been facing the threat of a variety of infectious diseases. Airborne microorganisms can cause airborne infectious diseases, which spread rapidly and extensively, causing huge losses to human society on a global scale. In recent years, the detection technology for airborne microorganisms has developed rapidly; it can be roughly divided into biochemical, immune, and molecular technologies. However, these technologies still have some shortcomings; they are time-consuming and have low sensitivity and poor stability. Most of them need to be used in the ideal environment of a laboratory, which limits their applications. A biosensor is a device that converts biological signals into detectable signals. As an interdisciplinary field, biosensors have successfully introduced a variety of technologies for bio-detection. Given their fast analysis speed, high sensitivity, good portability, strong specificity, and low cost, biosensors have been widely used in environmental monitoring, medical research, food and agricultural safety, military medicine and other fields. In recent years, the performance of biosensors has greatly improved, becoming a promising technology for airborne microorganism detection. This review introduces the detection principle of biosensors from the three aspects of component identification, energy conversion principle, and signal amplification. It also summarizes its research and application in airborne microorganism detection. The new progress and future development trend of the biosensor detection of airborne microorganisms are analyzed.

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“…The resulting biosensor was capable of detecting the 16 kDa HSP in concentrations as low as 100 fmol L −1 . The method covers a wide analytical range that extends from 100 fmol L −1 to 1 nmol L −1 …”

Section: Tropical Diseasesmentioning

confidence: 99%

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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Nanogapped impedimetric immunosensor for the detection of 16 kDa heat shock protein against Mycobacterium tuberculosis

Cited by 50 publications

References 32 publications

Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

Advances in airborne microorganisms detection using biosensors: A critical review

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

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