Nanofiltration Enrichment of Milk Oligosaccharides (MOS) in Relation to Process Parameters

Altmann, Karina; Clawin‐Rädecker, Ingrid; Hoffmann, Wolfgang; Lorenzen, Peter Christian

doi:10.1007/s11947-016-1763-5

Cited by 16 publications

(6 citation statements)

References 28 publications

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“…Oligosaccharides were produced according to Frenzel et al (2015) and Altmann et al (2015 ; 2016) . In brief, GOS was prepared using the enzyme HaLactase from K. lactis .…”

Section: Methodsmentioning

confidence: 99%

Microbiota independent effects of oligosaccharides on Caco-2 cells -A semi-targeted metabolomics approach using DI-FT-ICR-MS coupled with pathway enrichment analysis

Jensen-Kroll

Demetrowitsch²,

Clawin‐Rädecker³

et al. 2022

Front. Mol. Biosci.

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Milk oligosaccharides (MOS) and galactooligosaccharides (GOS) are associated with many benefits, including anti-microbial effects and immune-modulating properties. However, the cellular mechanisms of these are largely unknown. In this study, the effects of enriched GOS and MOS mixtures from caprine and bovine milk consisting mainly 6'-galactosyllactose, 3'-sialyllactose, and 6'-sialyllactose on Caco-2 cells were investigated, and the treatment-specific metabolomes were described. In the control, the cells were treated with a sugar mix consisting of one-third each of glucose, galactose and lactose.A local metabolomics workflow with pathway enrichment was established, which specifically addresses DI-FT-ICR-MS analyses and includes adaptations in terms of measurement technology and sample matrices. By including quality parameters, especially the isotope pattern, we increased the precision of annotation. The independence from online tools, the fast adaptability to changes in databases, and the specific adjustment to the measurement technology and biomaterial used, proved to be a great advantage.For the first time it was possible to find 71 active pathways in a Caco-2 cell experiment. These pathways were assigned to 12 main categories, with amino acid metabolism and carbohydrate metabolism being the most dominant categories in terms of the number of metabolites and metabolic pathways. Treatment of Caco-2 cells with high GOS and glucose contents resulted in significant effects on several metabolic pathways, whereas the MOS containing treatments resulted only for individual metabolites in significant changes. An effect based on bovine or caprine origin alone could not be observed. Thus, it was shown that MOS and GOS containing treatments can exert microbiome-independent effects on the metabolome of Caco-2 cells.

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“…Oligosaccharides were produced according to Frenzel et al (2015) and Altmann et al (2015 ; 2016) . In brief, GOS was prepared using the enzyme HaLactase from K. lactis .…”

Section: Methodsmentioning

confidence: 99%

Microbiota independent effects of oligosaccharides on Caco-2 cells -A semi-targeted metabolomics approach using DI-FT-ICR-MS coupled with pathway enrichment analysis

Jensen-Kroll

Demetrowitsch²,

Clawin‐Rädecker³

et al. 2022

Front. Mol. Biosci.

Self Cite

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“…Other than single‐stage nanofiltration after lactose hydrolysis, Altmann et al. (2016) implemented the combination of lactose hydrolysis and two‐stage nanofiltration to purify milk oligosaccharides from the bovine milk ultrafiltration permeate. The high recovery yield (90%) of BMOs was achieved under acidic (pH 5) conditions.…”

Section: Enrichment Of Bmos and Cmosmentioning

confidence: 99%

“…Moreover, the dry mass content of BMOs in the final nanofiltration retentate was four times higher than that in first nanofiltration retentate and 140 times higher than that in the ultrafiltration permeate. After two‐stage nanofiltration steps, the use of diafiltration caused most of lactose and monosaccharides in the final nanofiltration retentate to pass through the nanofiltration membrane, thereby increasing the BMO content relative to the total sugar (monosaccharides, lactose, and oligosaccharides) content from 30.5% to 92.1% (Altmann et al., 2016).…”

Section: Enrichment Of Bmos and Cmosmentioning

confidence: 99%

“…The purification of BMOs and CMOs from byproducts containing large amounts of lactose by single membrane filtration is still a challenging task. Until now, technological methods to recover milk oligosaccharides mainly utilized the combination of lactose hydrolysis and nanofiltration followed by diafiltration (Altmann et al, 2015(Altmann et al, , 2016Sarney et al, 2000). The lactose hydrolysis step before the nanofiltration process can achieve a considerable reduction of the remaining lactose concentration in the final enriched fraction and remarkably improve the efficiency of purification during nanofiltration processing (Sarney et al, 2000).…”

Section: 12mentioning

confidence: 99%

“…A major obstacle for isolating and purifying oligosaccharides from dairy processing byproducts is to remove simple sugars (lactose and monosaccharides) from all carbohydrates, thereby increasing the purity of milk oligosaccharides (de Moura Bell et al., 2018). Approaches to reduce the content of simple sugars include solid‐phase extraction (Robinson, Colet, et al., 2018; Robinson, Poulsen, et al., 2018; Ward, 2009), gel filtration chromatography (Marino et al., 2011; Urashima et al., 2013), charcoal column absorption chromatography (Brand‐Miller et al., 1998), and membrane‐based filtration (Altmann et al., 2016; Oliveira et al., 2012). Among them, membrane technology is considered the most feasible downstream strategy for industrial production.…”

Section: Introductionmentioning

confidence: 99%

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Membrane separation processes for enrichment of bovine and caprine milk oligosaccharides from dairy byproducts

Wang

JingHua

2021

Comp Rev Food Sci Food Safe

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Breast milk is an ideal source of human milk oligosaccharides (HMOs) for isolation and purification. However, breast milk is not for sale and at most is distributed to neonatal intensive care units as donor milk. To overcome this limitation, isolating HMOs analogs including bovine milk oligosaccharides (BMOs) and caprine milk oligosaccharides (CMOs) from other sources is timely and significant. Advances in the development of equipment and analytical methods have revealed that dairy processing byproducts are good sources of BMOs and CMOs. Enrichment of these oligosaccharides from dairy byproducts, such as whey, permeate, and mother liquor, is of increasing academic and economic value. The commonly employed approach for oligosaccharides purification is chromatographic technique, but it is only used at lab scale. In the dairy industry, chromatographic methods (large‐scale ion exchange, 10,000 L size) are currently routinely used for the isolation/purification of milk proteins (e.g., lactoferrin). In contrast, membrane technology has been proven to be a suitable approach for the isolation and purification of BMOs and CMOs from dairy byproducts. Therefore, this review simply introduces BMOs and CMOs in dairy processing byproducts. This review also summarizes membrane separation processes for isolating and purifying BMOs and CMOs from different dairy byproducts. Finally, the technological challenges and solutions of each processing strategy are discussed in detail.

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Concentration of Milk and Whey by Membrane Technologies in Alternative Cascade Modes

Meyer

Hartinger

Sigler

et al. 2016

Food Bioprocess Technol

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Nanofiltration Enrichment of Milk Oligosaccharides (MOS) in Relation to Process Parameters

Cited by 16 publications

References 28 publications

Microbiota independent effects of oligosaccharides on Caco-2 cells -A semi-targeted metabolomics approach using DI-FT-ICR-MS coupled with pathway enrichment analysis

Microbiota independent effects of oligosaccharides on Caco-2 cells -A semi-targeted metabolomics approach using DI-FT-ICR-MS coupled with pathway enrichment analysis

Membrane separation processes for enrichment of bovine and caprine milk oligosaccharides from dairy byproducts

Concentration of Milk and Whey by Membrane Technologies in Alternative Cascade Modes

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