Nanofibrous yet injectable polycaprolactone-collagen bone tissue scaffold with osteoprogenitor cells and controlled release of bone morphogenetic protein-2

Subramanian, Gayathri; Bialorucki, Callan; Yildirim-Ayan, Eda

doi:10.1016/j.msec.2015.02.030

Cited by 34 publications

(26 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To get more clear ideas, cell viability over PCL-NF matrix containing different percentage ratios of these antibacterial nanoparticles would be performed in the next possible study. Col-c-PCL promotes more cell growth than PCL-NF, because coating of collagen over any synthetic polymer nanofibers could enhance their biocompatibility (19). In this study, the % viability of cells in the presence of different electrospun matrixes was compared and calculated as the percentage relative to the untreated control cells (cells were not added in any nanofibrous matrix solution).…”

Section: Mtt Assaymentioning

confidence: 99%

“…The different synthetic polymers (11,12) such as polyglycolide (PGA) (13), polyvinyl pyrrolidone (PVP) (14), and their copolymers poly(lactide-co-glycolide) (PLGA) (15), poly(ε-caprolactone) (PCL) (16), and polyurethane (PU) (17) have been widely used in electrospinning process. Other natural polymers such as collagen, fibronectin, laminin, alginic acid, and chitosan can be also electrospun for producing nanofibrous scaffolds (18,19).…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, concepts of hybrid scaffolds have been started to avoid such limitations and use of both natural and synthetic polymers together to combine their good properties. When natural polymers such as collagen, fibronectin, laminin, alginic acid, and chitosan are electrospun and used alone, they can exhibit properties of the extracellular matrix (26) with excellent biocompatible surface enhancing the cell adhesion (18,19). However, such scaffolds are mechanically weak.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

et al. 2016

View full text Add to dashboard Cite

Abstract. In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO 2 -i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO 2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and Xray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO 2 -i-PCL due to collagen coating and incorporation of TiO 2 , respectively. EDX and XPS studies revealed distribution of entrapped TiO 2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

show abstract

Section: Mtt Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The newly formed osteoblasts in bone scaffolds with good osteogenesis have the potential for further mineralization and formation of new external bone construct. Therefore, many scientists and technician have improved the osteoinductivity of bone scaffolds, by changing their physical and chemical properties or by incorporating biological constitute into the scaffolds [12]. Moreover, the porosity and pore topology structure of bone scaffolds can be modified, to enhance the osteoconductivity [13,14].…”

Section: Osteoinductivity and Osteoconductivitymentioning

confidence: 99%

Application of 3D printing technology in bone tissue engineering

Wang

Wei

et al. 2018

Bio-des. Manuf.

View full text Add to dashboard Cite

“…Bone morphogenetic proteins (BMPs) are representative TGF-β superfamily growth factors which affect cell growth, migration and differentiation. They also act as potent regulators during embryogenesis and bone/cartilage formation and repair [1][2][3][4] . Among them, BMP-2 has been approved by FDA to be used in the clinical treatment of spine fusion, tibia fractures and dental grafts 5,6) .…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> study on the osteogenesis enhancement effect of BMP-2 incorporated biomimetic apatite coating on titanium surfaces

Zhu

Zhang

et al. 2017

Dent. Mater. J.

View full text Add to dashboard Cite

To fabricate a sustained-release delivery system of bone morphogenetic protein (BMP-2) on titanium surface, explore the effect of BMP-2 concentration on the loading/release behavior of BMP-2 and evaluate the cell compatibility of the system in vitro, pure titanium specimens were immersed into supersaturated calcium phosphate solutions (SCP) containing 4 different concentrations of BMP-2: 0, 50, 100, 200 and 400 ng/mL. Biomimetic calcium phosphate coating was formed on titanium surface and BMP-2 was incorporated into the coating through co-deposition. The release profile of BMP-2 suggested that BMP-2 were delivered sustainably up to 20 days. CCK-8 and ALP assay showed that 200 group and 400 ng/mL BMP-2 group have significant effect on promoting MC3T3-E1 cell proliferation and differentiation. The BMP-2 incorporated into the hybrid coating released in a sustained manner and significantly promoted the proliferation and differentiation of MC3T3-E1 on the titanium surface.

show abstract

Nanofibrous yet injectable polycaprolactone-collagen bone tissue scaffold with osteoprogenitor cells and controlled release of bone morphogenetic protein-2

Cited by 34 publications

References 49 publications

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

Application of 3D printing technology in bone tissue engineering

<i>In vitro</i> study on the osteogenesis enhancement effect of BMP-2 incorporated biomimetic apatite coating on titanium surfaces

Contact Info

Product

Resources

About