Nanofibrous polysaccharide hydroxyapatite composites with biocompatibility against human osteoblasts

Gašparič, Petra; Kurečič, Manja; Kargl, Rupert; Maver, Uroš; Gradišnik, Lidija; Hribernik, Silvo; Stana‐Kleinschek, Karin; Smole, Majda Sfiligoj

doi:10.1016/j.carbpol.2017.08.111

Cited by 23 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in tensile stress and Young modulus at low loading of EnHA nanoparticles may be due to the high alignment or orientation of the needled-liked particles on the PLA fiber mats. This is consistent with previous reports which showed that alignment of nanoplatelet hydroxyapatite on nylon electrospun fibers turns to improve their mechanical properties [17]. The good alignment of the EnHA particles on the surface of the fibers turns to promote good interfacial hydrogen bonding between the EnHA particles and the PLA polymer causing a good stress transfer from the matrix to the nanofibers [26].…”

Section: Resultssupporting

confidence: 92%

“…Polymer/HA scaffolds have been produced using different varieties of technologies such as extrusion, stereo lithography, coprecipitation, electrospinning, etc. However, the simplicity of experimental setup, low cost, the high porosity, and high interconnectivity of electrospun fibers makes them ideal tissue scaffolds [14–17]. HA nanoparticles are incorporated into the polymer matrix either through biomineralization of pristine polymer fiber mats or coelectrospinning of the polymer and the HA nanoparticles [4, 18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold

Apalangya

Rangari

Tiimob

et al. 2019

International Journal of Biomaterials

View full text Add to dashboard Cite

Nanocomposite electrospun fibers were fabricated from poly(lactic) acid (PLA) and needle-like hydroxyapatite nanoparticles made from eggshells. The X-ray diffraction spectrum and the scanning electron micrograph showed that the hydroxyapatite particles are highly crystalline and are needle-liked in shape with diameters between 10 and 20 nm and lengths ranging from 100 to 200 nm. The microstructural, thermal, and mechanical properties of the electrospun fibers were characterized using scanning electron microscope (SEM), thermogravimetric analysis (TGA), dynamic scanning calorimetry (DSC), and tensile testing techniques. The SEM study showed that both pristine and PLA/EnHA fibers surfaces exhibited numerous pores and rough edges suitable for cell attachment. The presence of the rod-liked EnHA particles was found to increase thermal and mechanical properties of PLA fibers relative to pristine PLA fibers. The confocal optical images showed that osteoblast cells were found to attach on dense pristine PLA and PLA/HA-10 wt% fibers after 48 hours of incubation. The stained confocal optical images indicated the secretion of cytoplasmic extension linking adjoining nuclei after 96 hours of incubation. These findings showed that eggshell based nanohydroxyapatite and poly(lactic acid) fibers could be potential scaffold for tissue regeneration.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold

Apalangya

Rangari

Tiimob

et al. 2019

International Journal of Biomaterials

View full text Add to dashboard Cite

show abstract

“…Various types of hydroxyapatite composites have been reported to have a role in osteoblast activity. Nanofibrous polysaccharide hydroxyapatite composites with biocompatibility maintained osteoblast survival and adhesion . Osteoblasts cultured on biocompatible poly( l ‐lactic acid)‐co‐poly (ε‐caprolactone)‐silk fibroin‐hydroxyapatite‐hyaluronic acid (PLACL–SF–HAp–HA) showed a significantly higher level of proliferation and increased osteogenic differentiation and mineralization .…”

Section: Discussionmentioning

confidence: 98%

Effect of hydroxyapatite nanoparticles and wedelolactone on osteoblastogenesis from bone marrow mesenchymal stem cells

Dong

Zhu

Deng

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Regenerative medicine has a high demand for the development of scaffold materials combined with other osteogenic inducers to generate bioactive composite materials for bone replacement therapies. Previously, we reported that wedelolactone promoted osteoblastogenesis of bone marrow mesenchymal stem cells (BMSCs). In this study, the effect of hydroxyapatites (HAps), bone composite materials we prepared, and the combined effect of wedelolactone and HAps on osteoblastogenesis differentiation was first evaluated. Three kinds of HAps constructed by a rod-like shape with particle size of 25 nm (HAp-1), 37 nm (HAp-2), and 33 nm (HAp-3) did not affect BMSC survival, but induced activity of alkaline phosphatase(ALP), a marker enzyme for osteoblastogenesis. HAp-1 treatment resulted in a more significant increase in the number of ALP staining-positive BMSC, and maintained an extended time for the increased number of ALP stainingpositive BMSC. Moreover, HAp-1 combined with wedelolactone induced a higher ALP activity for a longer time than HAp-2 and HAp-3, and also increased the bone mineralization level. Osteoblastogenesis-related marker genes expression including osteorix, osteocalcin, and runx2 were increased after BMSC were treated with HAp-1 for 6 days. Although three kinds of HAps treatment for 9 days increased osteorix mRNA expression, osteocalcin, and runx2 mRNA expression levels were upregulated only by HAp-1. Similarly, only HAp-1 enhanced wedelolactone-induced osteocalcin, osteorix, and runx2 mRNA expression after 9 days treatment. Together, these results suggested that HAps with different sizes generated different effect on osteoblastogenesis. HAp-1 combined with wedelolacone can exert an enhanced effect on osteoblastogenesis, which has potential for treating osteoporosis.

show abstract

Section: Natural Polymer Nanofibersmentioning

confidence: 99%

“…They cultured MC3T3-E1 osteoblast cells on the scaffold and reported good results using an MTT assay and scanning electron microscopy (SEM) for evaluation. In addition, Gasparic et al produced a nanoparticle composite of cellulose and HA using the electrospinning method [60]. The electrospun nanofibers were several hundred nanometers in diameter and human-bone-derived osteoblasts were cultured on the scaffold, which demonstrated cell proliferation by MTT assay.…”

Section: Natural Polymer Nanofibersmentioning

confidence: 99%

The Use of Electrospun Organic and Carbon Nanofibers in Bone Regeneration

2020

View full text Add to dashboard Cite

There has been an increasing amount of research on regenerative medicine for the treatment of bone defects. Scaffolds are needed for the formation of new bone, and various scaffolding materials have been evaluated for bone regeneration. Materials with pores that allow cells to differentiate into osteocytes are preferred in scaffolds for bone regeneration, and porous materials and fibers are well suited for this application. Electrospinning is an effective method for producing a nanosized fiber by applying a high voltage to the needle tip containing a polymer solution. The use of electrospun nanofibers is being studied in the medical field, and its use as a scaffold for bone regeneration therapy has become a topic of growing interest. In this review, we will introduce the potential use of electrospun nanofiber as a scaffold for bone regenerative medicine with a focus on carbon nanofibers produced by the electrospinning method.

show abstract

Nanofibrous polysaccharide hydroxyapatite composites with biocompatibility against human osteoblasts

Cited by 23 publications

References 33 publications

Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold

Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold

Effect of hydroxyapatite nanoparticles and wedelolactone on osteoblastogenesis from bone marrow mesenchymal stem cells

The Use of Electrospun Organic and Carbon Nanofibers in Bone Regeneration

Contact Info

Product

Resources

About