Nanofibrous nerve guidance conduits decorated with decellularized matrix hydrogel facilitate peripheral nerve injury repair

Zheng, Chushan; Yang, Zehong; Chen, Shihao; Zhang, Fang; Rao, Zilong; Zhao, Cailing; Bai, Ying; Shen, Jun

doi:10.7150/thno.50825

Cited by 63 publications

(52 citation statements)

References 53 publications

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“…An example of this combination is where NGCs made of aligned PLLA nanofibers were incorporated with porcine decellularized nerve matrix hydrogel (pDNM gel). When used to repair rat sciatic nerve defect models, the combination NGC resulted in an increased number of axons, extensive myelination and improved sciatic nerve function in both in-vitro and in-vivo tests [38]. In a recent investigation into developing an NGC that can control neural cell directional growth, aligned nanofibers made of PVA, gelatine and gellan were further processed to make a patterned hybrid scaffold which was then incorporated with quercetin to obtain functional contact guidance for neural cells.…”

Section: Combination Of Stimulating Strategiesmentioning

confidence: 99%

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

2022

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Injuries to the peripheral nervous system result in devastating consequences with loss of motor and sensory function and lifelong impairments. Current treatments have largely relied on surgical procedures, including nerve autografts to repair damaged nerves. Despite improvements to the surgical procedures over the years, the clinical success of nerve autografts is limited by fundamental issues, such as low functionality and mismatching between the damaged and donor nerves. While peripheral nerves can regenerate to some extent, the resultant outcomes are often disappointing, particularly for serious injuries, and the ongoing loss of function due to poor nerve regeneration is a serious public health problem worldwide. Thus, a successful therapeutic modality to bring functional recovery is urgently needed. With advances in three-dimensional cell culturing, nerve guidance conduits (NGCs) have emerged as a promising strategy for improving functional outcomes. Therefore, they offer a potential therapeutic alternative to nerve autografts. NGCs are tubular biostructures to bridge nerve injury sites via orienting axonal growth in an organized fashion as well as supplying a supportively appropriate microenvironment. Comprehensive NGC creation requires fundamental considerations of various aspects, including structure design, extracellular matrix components and cell composition. With these considerations, the production of an NGC that mimics the endogenous extracellular matrix structure can enhance neuron–NGC interactions and thereby promote regeneration and restoration of function in the target area. The use of electrospun fibrous substrates has a high potential to replicate the native extracellular matrix structure. With recent advances in electrospinning, it is now possible to generate numerous different biomimetic features within the NGCs. This review explores the use of electrospinning for the regeneration of the nervous system and discusses the main requirements, challenges and advances in developing and applying the electrospun NGC in the clinical practice of nerve injuries.

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Section: Combination Of Stimulating Strategiesmentioning

confidence: 99%

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

2022

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“…1 ). Chemical detergent has a good effect on removing genetic material from tissues, which has been recognized by most researchers [ 33 , 71 ]. In this study, the DNA content of porcine sciatic nerves treated with SDS decreased from 3725.73 ± 242.31 ng/mg to 52.28 ± 3.58 ng/mg ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, hydrogels have attracted much attention due to their excellent properties, especially xenogeneic acellular matrix hydrogels, which have been used to repair various types of tissue injury [ [79] , [80] , [81] , [82] ]. On a related note, in the repair of peripheral nerve injury, there are also several reports on heterogeneous acellular matrix hydrogels [ 71 , 74 ]. However, we believe that in the repair of peripheral nerve injury, especially nerve defects, acellular nerve scaffolds have more advantages than hydrogels because well-preserved three-dimensional directional nerve fiber basement membrane tubes provide nanotopological clues for regenerated axons.…”

Section: Discussionmentioning

confidence: 99%

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Wei

et al. 2022

Bioactive Materials

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“…Some anisotropic hydrogel scaffolds for nerve regeneration were based on aligned fibrous structures [ 146 , 189 , 190 ]. Topographical cues of fibers and soft, hydrophilic features of hydrogels were combined to better mimic axon structures.…”

Section: Anisotropic 3d Hydrogel Scaffoldsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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Nanofibrous nerve guidance conduits decorated with decellularized matrix hydrogel facilitate peripheral nerve injury repair

Cited by 63 publications

References 53 publications

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

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