Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Wei, Shuai; Hu, Qian; Ma, Jianxiong; Dai, Xiu; Sun, Yu; Han, Gonghai; Meng, Hui; Xu, Wei; Zhang, Lei; Ma, Xinlong; Peng, Jiang; Wang, Yu

doi:10.1016/j.bioactmat.2022.03.014

Cited by 11 publications

(10 citation statements)

References 98 publications

(125 reference statements)

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“…It is hypothesized that ScCO2 might induce hypoxia, which has been validated by histological and morphological analyses after successful decellurization of bovine optic nerves using ScCO2 ( Topuz et al, 2020 ). Wei et al developed a porcine acellular nerve xenograft based on supercritical extraction technology and validated it in a 15-mm rat sciatic nerve model ( Wei et al, 2022 ). A porous nerve basement membrane with a well-preserved 3D structure was observed.…”

Section: Different Approaches Of Physical Processingmentioning

confidence: 99%

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Hsu

Chen

Tseng

et al. 2023

Front. Bioeng. Biotechnol.

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In severe or complex cases of peripheral nerve injuries, autologous nerve grafts are the gold standard yielding promising results, but limited availability and donor site morbidity are some of its disadvantages. Although biological or synthetic substitutes are commonly used, clinical outcomes are inconsistent. Biomimetic alternatives derived from allogenic or xenogenic sources offer an attractive off-the-shelf supply, and the key to successful peripheral nerve regeneration focuses on an effective decellularization process. In addition to chemical and enzymatic decellularization protocols, physical processes might offer identical efficiency. In this comprehensive minireview, we summarize recent advances in the physical methods for decellularized nerve xenograft, focusing on the effects of cellular debris clearance and stability of the native architecture of a xenograft. Furthermore, we compare and summarize the advantages and disadvantages, indicating the future challenges and opportunities in developing multidisciplinary processes for decellularized nerve xenograft.

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Section: Different Approaches Of Physical Processingmentioning

confidence: 99%

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Hsu

Chen

Tseng

et al. 2023

Front. Bioeng. Biotechnol.

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“…The CatWalk footprint machine (XT 10.6, Noldus, Netherlands) was used to assess the locomotion of the rats following surgery 54 . Prior to testing, the rats were trained to become familiar with the footprint recording platform.…”

Section: Assessment Of Functional Recoverymentioning

confidence: 99%

3D Printing rGO/PCL/Mel Bionic Conduit for Peripheral Nerve Regeneration

Zhang

Liu

et al. 2023

Preprint

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The inevitable secondary victimization of patients during the grafting of autogenous nerve necessitates the urgent development of bioactive conduits for the precise repair of peripheral nerve (PN) defects. However, the limited selection of appropriate components and inferior structural designs of many porous scaffolds have hindered satisfactory PN regeneration. In this study, we created a 3D hollow conduit of reduced graphene oxide (rGO) with a hierarchically ordered microstructure through a coaxial printing methodology that enabled a physicochemically cooperative construction process at multiscale. We deposited a mixture of polycaprolactone (PCL) and melatonin (Mel) as the biologically enhancing constitution conformably over the 3D rGO templated conduit. Attributing to its elaborately designed hierarchical structure and arched alignment of 2D micro sheets, the 3D rGO/PCL/Mel hybrid bio-conduit has demonstrated remarkable structural robustness in maintaining ordered pathways and high porosity (98.5 ± 0.24%), which facilitated nerve growth in a complex survival environment in vivo. Furthermore, the excellent combination of properties such as electrical conductivity, biocompatibility, and mechanical properties (with an elastic modulus ranging from 7.06 ± 0.81 MPa to 26.58 ± 4.99 MPa), has led to highly efficient regeneration of well-ordered PN tissue. Systematic evaluations of nerve regeneration and muscle function recovery in an SD rat model with a long nerve defect (> 15 mm) have validated the virtually identical performance of the 3D rGO/PCL/Mel conduit compared to the autogenous nerve graft group. This study confirms a promising approach to clinical PN repair of long defects through the combined regulation of rational structure design on multiscale and indispensable chemical modification of rGO-based functional nerve regeneration conduits.

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“…11 The success of nerve repair relies on creating an optimal regenerative microenvironment. 12 Within the nerve conduit, an ideal intracavitary microenvironment should encompass crucial factors for nerve regeneration, including immune response modulation, angiogenesis promotion, and signaling molecule regulation. 12,13 By providing these components, the artificial nerve conduit aims to facilitate nerve regeneration and functional recovery.…”

Section: Introductionmentioning

confidence: 99%

“…To overcome these limitations, researchers have developed artificial nerve conduits that hold the potential to replace autologous nerve transplantation as the preferred treatment standard in the future 11 . The success of nerve repair relies on creating an optimal regenerative microenvironment 12 . Within the nerve conduit, an ideal intracavitary microenvironment should encompass crucial factors for nerve regeneration, including immune response modulation, angiogenesis promotion, and signaling molecule regulation 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Combining PLGA microspheres loaded with Liver X receptor agonist GW3965 with a chitosan nerve conduit can promote the healing and regeneration of the wounded sciatic nerve

Zhong,

Li,

Chen

et al. 2024

J Biomed Mater Res

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Globally, peripheral nerve injury (PNI) is a common clinical issue. Successfully repairing severe PNIs has posed a major challenge for clinicians. GW3965 is a highly selective LXR agonist, and previous studies have demonstrated its positive protective effects in both central and peripheral nerve diseases. In this work, we examined the potential reparative effects of GW3965‐loaded polylactic acid co‐glycolic acid microspheres in conjunction with a chitosan nerve conduit for peripheral nerve damage. The experiment revealed that GW3965 promoted Schwann cell proliferation and neurotrophic factor release in vitro. In vivo experiments conducted on rats showed that GW3965 facilitated the restoration of motor function, promoted axon and myelin regeneration in the sciatic nerve, and enhanced the microenvironment of nerve regeneration. These results offer a novel therapeutic approach for the healing of nerve damage. Overall, this work provides valuable insights and presents a promising therapeutic strategy for addressing PNI.

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Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

Cited by 11 publications

References 98 publications

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

3D Printing rGO/PCL/Mel Bionic Conduit for Peripheral Nerve Regeneration

Combining PLGA microspheres loaded with Liver X receptor agonist GW3965 with a chitosan nerve conduit can promote the healing and regeneration of the wounded sciatic nerve

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