Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Hsu, Miao Hsing; Chen, Szu-Han; Tseng, Wan‐Ling; Hung, Kuo-Shu; Chung, Tzu-Chun; Lin, Shio‐Jean; Koo, Jahyun; Hsueh, Yuan Yu

doi:10.3389/fbioe.2023.1217067

Cited by 3 publications

(2 citation statements)

References 56 publications

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“…This research is crucial for advancing the treatment of severe nerve injuries where traditional methods may not be sufficient ( Garcia-Garcia et al, 2023 ). Decellularized nerve grafts should provide a suitable substrate for nerve regeneration in which the conservation of ECM components plays a crucial role for peripheral nerve regeneration and axonal guidance ( Hsu et al, 2023 ; Mahdian et al, 2023 ). The currently available decellularized methods are time and effort consuming ( El Soury et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Muratori,

Crosio,

Ronchi

et al. 2024

Front. Neuroanat.

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IntroductionPeripheral nerves are frequently affected by lesions caused by traumatic or iatrogenic damages, resulting in loss of motor and sensory function, crucial in orthopedic outcomes and with a significant impact on patients’ quality of life. Many strategies have been proposed over years to repair nerve injuries with substance loss, to achieve musculoskeletal reinnervation and functional recovery. Allograft have been tested as an alternative to the gold standard, the autograft technique, but nerves from donors frequently cause immunogenic response. For this reason, several studies are focusing to find the best way to decellularize nerves preserving either the extracellular matrix, either the basal lamina, as the key elements used by Schwann cells and axons during the regenerative process.MethodsThis study focuses on a novel decellularization protocol for porcine nerves, aimed at reducing immunogenicity while preserving essential elements like the extracellular matrix and basal lamina, vital for nerve regeneration. To investigate the efficacy of the decellularization protocol to remove immunogenic cellular components of the nerve tissue and to preserve the basal lamina and extracellular matrix, morphological analysis was performed through Masson’s Trichrome staining, immunofluorescence, high resolution light microscopy and transmission electron microscopy. Decellularized porcine nerve graft were then employed in vivo to repair a rat median nerve lesion. Morphological analysis was also used to study the ability of the porcine decellularized graft to support the nerve regeneration.Results and DiscussionThe decellularization method was effective in preparing porcine superficial peroneal nerves for grafting as evidenced by the removal of immunogenic components and preservation of the ECM. Morphological analysis demonstrated that four weeks after injury, regenerating fibers colonized the graft suggesting a promising use to repair severe nerve lesions. The idea of using a porcine nerve graft arises from a translational perspective. This approach offers a promising direction in the orthopedic field for nerve repair, especially in severe cases where conventional methods are limited.

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Section: Discussionmentioning

confidence: 99%

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Muratori,

Crosio,

Ronchi

et al. 2024

Front. Neuroanat.

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“…This review article specifically focuses on nerve regeneration using decellularized nerve tissues (DNT). In recent studies, the use of biological scaffolds has been the focus of researchers in nerve repair ( Hsu et al, 2023 ; Yu et al, 2023 ). Therefore, we decided to mention this issue in this paper, and we hope that we have helped those interested in this field.…”

Section: Introductionmentioning

confidence: 99%

Nerve regeneration using decellularized tissues: challenges and opportunities

Mahdian,

Tabatabai,

Abpeikar

et al. 2023

Front. Neurosci.

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In tissue engineering, the decellularization of organs and tissues as a biological scaffold plays a critical role in the repair of neurodegenerative diseases. Various protocols for cell removal can distinguish the effects of treatment ability, tissue structure, and extracellular matrix (ECM) ability. Despite considerable progress in nerve regeneration and functional recovery, the slow regeneration and recovery potential of the central nervous system (CNS) remains a challenge. The success of neural tissue engineering is primarily influenced by composition, microstructure, and mechanical properties. The primary objective of restorative techniques is to guide existing axons properly toward the distal end of the damaged nerve and the target organs. However, due to the limitations of nerve autografts, researchers are seeking alternative methods with high therapeutic efficiency and without the limitations of autograft transplantation. Decellularization scaffolds, due to their lack of immunogenicity and the preservation of essential factors in the ECM and high angiogenic ability, provide a suitable three-dimensional (3D) substrate for the adhesion and growth of axons being repaired toward the target organs. This study focuses on mentioning the types of scaffolds used in nerve regeneration, and the methods of tissue decellularization, and specifically explores the use of decellularized nerve tissues (DNT) for nerve transplantation.

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Advancements in stimulation therapies for peripheral nerve regeneration

Bordett,

Danazumi,

Wijekoon

et al. 2024

Biomed. Mater.

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Soft-tissue injuries affecting muscles, nerves, vasculature, tendons, and ligaments often diminish the quality of life due to pain, loss of function, and financial burdens. Both natural healing and surgical interventions can result in scarring, which potentially may impede functional recovery and lead to persistent pain. Scar tissue, characterized by a highly disorganized fibrotic extracellular matrix (ECM), may serve as a physical barrier to regeneration and drug delivery. While approaches such as drugs, biomaterials, cells, external stimulation, and other physical forces show promise in mitigating scarring and promoting regenerative healing, their implementation remains limited and challenging. Ultrasound, laser, electrical, and magnetic forms of external stimulation have been utilized to promote soft tissue as well as neural tissue regeneration. After stimulation, neural tissues experience increased proliferation of Schwann cells, secretion of neurotropic factors, production of myelin, and growth of vasculature, all aimed at supporting axon regeneration and innervation. Yet, the outcomes of healing vary depending on the pathophysiology of the damaged nerve, the timing of stimulation following injury, and the specific parameters of stimulation employed. Increased treatment intensity and duration have been noted to hinder the healing process by inducing tissue damage. These stimulation modalities, either alone or in combination with nerve guidance conduits and scaffolds, have been demonstrated to promote healing. However, the literature currently lacks a detailed understanding of the stimulation parameters used for nerve healing applications. In this article, we aim to address this gap by summarizing existing reports and providing an overview of stimulation parameters alongside their associated healing outcomes.

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Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Cited by 3 publications

References 56 publications

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Nerve regeneration using decellularized tissues: challenges and opportunities

Advancements in stimulation therapies for peripheral nerve regeneration

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