to drive many small electronic devices or serve as self-powered sensors. [11][12][13][14] Its rapid development is reshaping our inherent understanding of energy harvesting technologies.As an emerging power in the field of selfpowered technology, the wearable TENGs are showing vigorous development vitality and giant application potential due to its simple configuration, light weight, low cost, output stability, and eco-friendly feature. [15,16] However, wearable devices will generally direct contact on the skin surface of the human body. While ensuring the stable output, the wearable TENG also requires excellent flexibility, portability, and breathability to improve human comfort during long-term use. [17][18][19][20] The traditional wearable TENGs are mostly assembled from the dense films that involve complex physical and chemical processes, which results in a high manufacturing cost and is hard to balance the physiological needs of human comfort. [21][22][23][24][25] There are still challenges in developing a wearable TENG that combines flexibility and breathability.The electrospun technique is one of the feasible preparation routes for TENG that assembled from the nanofiber films. [26][27][28][29][30][31] As an ideal triboelectric material, the nanofiber is attracting extensive attention due to its high specific surface area and excellent flexibility. Moreover, the porous structure gives it good breathability and ultra-lightweight. [32][33][34][35][36] And the nanofiber can be electrospun from various polymer materials, while the modification method and structural design are simple and diverse. [37,38] In addition, the Janus films could be fabricated via the electrospun method, which will combinate the advantages of Janus structure and electrospun nanofiber. The Janus films will provide asymmetric properties on each side with its own integrity (such as morphology, conductivity, and hydrophilicity). [39] Therefore, the dual-functional nanofiber films exhibit various potential applications, especially in the field of wearable electronics. [40][41][42][43] It is interesting and meaningful to fabricate a flexible and breathable TENG by the Janus films, the nanofiberbased TENG can be used as a wearable health-monitoring system or a self-powered supply source to directly attach at the skin surface and provide long-term comfort.Here, we first developed an all-nanofiber-based wearable TENG which is completely constructed from the Janus nanofiber films for energy harvesting and self-powered sensing.The traditional wearable nanogenerators are mostly assembled from the dense films that involve complex physical and chemical processes, which results in a high manufacturing cost and is hard to balance the physiological needs of human comfort. Herein, a wearable triboelectric nanogenerator (TENG) is developed that is completely constructed from the Janus electrospun nanofiber films. This all-nanofiber-based TENG not only has ultra-lightweight and high flexibility but also exhibits excellent breathability due to its porous st...