Nanofiber-hydrogel composite–mediated angiogenesis for soft tissue reconstruction

Li, Xiaowei; Cho, Brian; Martin, Russell; Seu, Michelle; Zhang, Chi; Zhou, Zhengbing; Choi, Ji Suk; Jiang, Xuesong; Chen, Long; Walia, Gurjot S.; Yan, Jerry; Callanan, Megan; Liu, Huanhuan; Colbert, Kevin; Morrissette‐McAlmon, Justin; Grayson, Warren L.; Reddy, Sashank K.; Sacks, Justin M.; Mao, Hai‐Quan

doi:10.1126/scitranslmed.aau6210

Cited by 187 publications

(171 citation statements)

References 49 publications

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“…Hydrogels are composed of three-dimensional molecular networks that contain a large amount of water 17,18 . Hydrogels provide high biocompatibility and a moist healing environment for skin wounds that promotes tissue repair and regeneration 19,20 . Chitosan has inherent antimicrobial activity and contains a large number of amino groups that can be chemically modified and crosslinked to produce antimicrobial hydrogels 21,22 .…”

Section: Introductionmentioning

confidence: 99%

An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

Sun

et al. 2020

NPG Asia Mater

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Currently, wound infection is an important health problem for the public. Wound infection can not only hinder healing but it can also lead to serious complications. Injectable wound dressings with biocompatible and antibacterial properties can promote wound healing during skin infections and reduce antibiotic use. Here, we used glycidyl methacrylate (GMA) to modify ε-polylysine (ε-PL) and γ-poly(glutamic acid) (γ-PGA) to produce ε-polylysine-glycidyl methacrylate (ε-PL-GMA) and γ-poly(glutamic acid)-glycidyl methacrylate (γ-PGA-GMA). Subsequently, ε-PL-GMAand γ-PGA-GMA-based hydrogels were developed through photopolymerization using visible light. The hydrogels were injectable, could rapidly gelatinize, were biocompatible, and showed a wide spectrum of antibacterial activity. The hydrogels also promoted wound healing. The results show that these hydrogels inhibit bacterial infection and shorten the wound healing time of skin defects in Staphylococcus aureus models. This demonstrates that the hydrogels hold potential for clinical antimicrobial and wound healing therapy.

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Section: Introductionmentioning

confidence: 99%

An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

Sun

et al. 2020

NPG Asia Mater

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“…Biomaterials and devices implanted in the body have a broad spectrum of clinical applications including tissue regeneration (1), cell transplantation (2), controlled drug release (3), continuous monitoring of physiological conditions (4) and electronic pacing (5).…”

Section: Introductionmentioning

confidence: 99%

Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing

Hu¹,

Chu²,

Liu

et al. 2020

Preprint

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Structural properties of biomaterials play critical roles in guiding cell behaviors and influence the immune response against them. In this study, we fabricated electro-spun membranes with three types of surface topography (Random, Aligned, and Latticed). The aligned membranes showed immunomodulatory ability, and led to faster wound healing, reduced fibrotic response and enhanced regeneration of cutaneous appendages. Based on that, we performed single-cell RNA sequencing analysis on cells of wounded mouse skin in the presence/absence of the Aligned scaffold. We identified 45 cell populations. Keratinocytes, fibroblasts, and immune cells including neutrophils, monocytes, macrophages, dendritic cells, and T cells showed diverse cellular heterogeneity. We found more inner root sheath cells (anagen-related) in the Aligned group, which corresponded to the improved regeneration of hair follicles in the presence of scaffold. The immune microenvironment around the biomaterial involved intricate interplay of immune cells from both innate and adaptive immune system. Immune responses in tissue around scaffold significantly differed from that of saline control. In aligned samples, infiltrated macrophages and neutrophils were reduced, whereas more effector T cells were recruited. The time course of immune response might be advanced towards an adaptive immunity-dominant stage by scaffolds.

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“…Fibrin glue with FGF-1 and heparin ePTFE Increasing capillarization, increased collagen content [235] P15 peptide based ePTFE Smaller thicknesses of neointimal hyperplasia; enhanced endothelialization [236] Growth factor -reduced Matrigel™-containing VEGF ePTFE Increased EC rate, increased myointimal hyperplasia [237] Hyaluronic acid based PCL Higher bulk porosity and cell permeability [238] VEGF based PEA, PMA Promoting vascularization in regenerative medicine applications [159] Platelet-rich plasma PLA/GTNF Healing improvement [240] (γ-glycidoxypropyltrimethoxysilane (GPTMS) Chitosan Improving mechanical strength [241] Poly-L-lysine, poly-L-ornithine, laminin, collagen Poly (sialic acid) Mechano compatibility; cell adhesive property [242] Collagen type I; EDC/NHS activation (amino groups) P(MMA-co-AA) Promotion of cell attachment, spreading, and viability [243] Polypyrrole (PPy) PCL, PLA Cell density, cell spread, [244,245] RGD peptide based PEG-heparin hybrid gel Differentiation & propagation of NSCs; outgrowth of axon dendrites [245,246] Laminin based Collagen type I Bridging peripheral nerve gaps [245,247] Visceral (Section 6) Lung (Section 6.1)…”

Section: Blood Vessels (Section 4)mentioning

confidence: 99%

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

2020

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In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are two-fold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.Polymers 2020, 12, 620 2 of 30 allows to tailor functionalize the coatings, where their responsiveness to external stimuli is one of their biggest advantages.Polymers stand out as a special class of materials due to the flexibility of design of their blocks and various functionalities, which is brought by their versatile assembly or by introducing additional side-groups or blocks, in the case of block-co-polymers. In this regard, both synthetic and biocompatible polymers are available, and polymer engineering represents a growing area tailoring the needs often driven by applications. With all advantages and the flexibility of the design, there is one significant weakness of polymers-the softness. In regard with tissue engineering, that translates to the fact that some materials can match predominantly soft tissue, but it is difficult to tune mechanical properties of polymers to match the hardness of bones, tendons, etc. And since mechanical properties of materials affect and even drive cell and tissue growth, enhancement of mechanical properties of polymers and biomaterials is essential. Chemical crosslinking can be applied to address these challenges, but that solves problems only partially.To solve these challenges the so-called hybrid materials [6] are used, which possess both inorganic and organic contents. Increasingly, hybrid materials are used in designing the extracellular matrix. The hybrid matrix design for various tissue engineering applications should meet bio-medical requirements. On the one hand, it needs to be biocompatible and biodegradable, which is achieved through the po...

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Nanofiber-hydrogel composite–mediated angiogenesis for soft tissue reconstruction

Cited by 187 publications

References 49 publications

An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

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