Nanofiber composites in gene delivery

priya, M. Lakshmi; Rana, Deepti; Bhatt, Abhisheik; Ramalingam, Murugan

doi:10.1016/b978-0-08-100173-8.00031-4

Cited by 8 publications

(3 citation statements)

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“…Introduction of DNA into the stem cells can signal the differentiation of cells into cells of interests (Figure 5) [110–112]. The insertion of DNA strand (gene) into a cell can be achieved by viral vectors and non-viral vectors [113].…”

Section: Functional Fibers For Stem Cell Therapymentioning

confidence: 99%

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The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

2019

Med One

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Stem cell therapy has emerged as one of the topics in tissue engineering where undifferentiated and multipotent cells are strategically placed/ injected in tissue structure for cell regeneration. Over the years, stem cells have shown promising results in skin repairs for non-healing and/or chronic wounds. The addition of the stem cells around the wound site promotes signaling pathways for growth factors that regulate tissue reconstruction. However, injecting stem cells around the wound site has its drawbacks, including cell death due to lack of microenvironment cues. This particular issue is resolved when biomaterial scaffolds are involved in the cultivation and mechanical support of the stem cells. In this review, we describe the current models of stem cell therapy by injections and those that are done through cell cultures using electrospun fiber scaffolds. Electrospun fibers are considered as an ideal candidate for cell cultures due to their surface properties. Through the control of fiber morphology and fiber structure, cells are able to proliferate and differentiate into keratinocytes for skin tissue regeneration. Furthermore, we provide another perspective of using electrospun fibers and stem cells in a layer-by-layer structure for skin substitutes (dressing). Finally, electrospun fibers have the potential to incorporate bioactive agents to achieve controlled release properties, which is beneficial to the survival of the delivered stem cells or the recruitment of the cells. Overall, our work illustrates that electrospun fibers are ideal for stem cell cultures while serving as cell carriers for wound dressing materials.

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Section: Functional Fibers For Stem Cell Therapymentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

The Role of Electrospun Fiber Scaffolds in Stem Cell Therapy for Skin Tissue Regeneration

2019

Med One

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“…Unlike targeted or conventional drug therapy, gene-based therapy acts at the DNA or mRNA level to intentionally modulate gene expression in specific cells for preventive or therapeutic actions through correcting gene transcription and translation processes [1], affording long-lasting and curative benefits in the treatment of various inherited and acquired diseases [2][3][4]. Considering that gene-based therapy implies the introduction of a functional tunable therapeutic gene to directly repair/amend or replace the altered genetic material at the molecular level [5,6], this unique approach may facilitate the modulation of genetic information through the exogenous stimulation of key signaling pathways in targeted cells, attaining various intended functions (e.g., the differentiation of certain cells into specialized cells, the production of cellular therapeutics or the stimulation of the apoptosis process in cancer cells) and offering innovative approaches for improving the targeted functions as well as fostering both the advancement of new therapeutics based on cell gene correction and their clinical translation [4,7].…”

Section: Introductionmentioning

confidence: 99%

Electrospun-Fibrous-Architecture-Mediated Non-Viral Gene Therapy Drug Delivery in Regenerative Medicine

2022

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Gene-based therapy represents the latest advancement in medical biotechnology. The principle behind this innovative approach is to introduce genetic material into specific cells and tissues to stimulate or inhibit key signaling pathways. Although enormous progress has been achieved in the field of gene-based therapy, challenges connected to some physiological impediments (e.g., low stability or the inability to pass the cell membrane and to transport to the desired intracellular compartments) still obstruct the exploitation of its full potential in clinical practices. The integration of gene delivery technologies with electrospun fibrous architectures represents a potent strategy that may tackle the problems of stability and local gene delivery, being capable to promote a controlled and proficient release and expression of therapeutic genes in the targeted cells, improving the therapeutic outcomes. This review aims to outline the impact of electrospun-fibrous-architecture-mediated gene therapy drug delivery, and it emphatically discusses the latest advancements in their formulation and the therapeutic outcomes of these systems in different fields of regenerative medicine, along with the main challenges faced towards the translation of promising academic results into tangible products with clinical application.

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