Nanofiber Alignment Regulates NIH3T3 Cell Orientation and Cytoskeletal Gene Expression on Electrospun PCL+Gelatin Nanofibers

Fee, Timothy; Surianarayanan, Swetha; Downs, J. Crawford; Zhou, Yong; Berry, Joel L.

doi:10.1371/journal.pone.0154806

Cited by 60 publications

(51 citation statements)

References 32 publications

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“…After applying a fold-change magnitude threshold (> 20%) and statistical significance threshold, eight of forty-eight genes associated with actin production, actin polymerization and focal adhesion formation were found to be significantly upregulated. [133] Taken together cell adhesion, spreading/elongation, alignment and migration in response to topographic cues displayed by anisotropic scaffolds are mediated by a complex interplay of factors including, but not limited to, focal adhesions, cytoskeletal elements, filopodia-mediated activities and traction forces. Generally speaking, it appears that design of scaffolds for skeletal muscle tissue engineering should feature topography that arranges cells to maximize cell-substrate contact and optimize cell organization to promote cell-cell interactions.…”

Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

2016

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Repair of damaged skeletal muscle tissue is limited by the regenerative capacity of native tissue. Current clinical approaches are not optimal for treatment of large volumetric skeletal muscle loss. As an alternative, tissue engineering represents a promising approach for the functional restoration of damaged muscle tissue. A typical tissue engineering process involves the design and fabrication of a scaffold that closely mimics the native skeletal muscle extracellular matrix allowing for organization of cells into a physiologically relevant, 3D architecture. In particular, anisotropic materials, which mimic the morphology of the native skeletal muscle ECM, can be fabricated using various biocompatible materialsto guide cell alignment, elongation, proliferation and differentiation into myotubes. In this article, we first provide an overview of fundamental concepts associated with muscle tissue engineering and the current status of the muscle tissue engineering approaches. We then review recent advances in development of anisotropic scaffolds with micro- or nano-scale features and examine how scaffold topographical, mechanical, and biochemical cues correlate to observed cellular function and phenotype development. Finally, we highlight some recent developments in both the design and utility of anisotropic materials in skeletal muscle tissue engineering along with their potential impact on future research and clinical application.

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Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

2016

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“…In vivo , newly formed bone was observed over 4 weeks, and the defect was covered after 12 weeks. Fibroblasts (NIH3T3) on the oriented nanofiber scaffold were elongate and aligned parallel to the fibers, and their gene expression upregulated associated with actin production, action polymerization, and focal adhesion formation than random one . Human mesenchymal stem cells (hMSC) on PLLA aligned nano‐fibermats were highly oriented in the direction of the collector, where the cells were stretched along the long axis of the nanofibers .…”

Section: Introductionmentioning

confidence: 99%

“…Fibroblasts (NIH3T3) on the oriented nanofiber scaffold were elongate and aligned parallel to the fibers, and their gene expression upregulated associated with actin production, action polymerization, and focal adhesion formation than random one. 22 Human mesenchymal stem cells (hMSC) on PLLA aligned nano-fibermats were highly oriented in the direction of the collector, where the cells were stretched along the long axis of the nanofibers. 23,24 Subsequently, the collagen fibril bundles produced by hMSC were aligned in the direction of the cell adhesion (i.e., the nano-fiber direction).…”

Section: Introductionmentioning

confidence: 99%

Development of bifunctional oriented bioactive glass/poly(lactic acid) composite scaffolds to control osteoblast alignment and proliferation

Lee

Matsugaki

Kasuga

et al. 2019

J Biomedical Materials Res

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During the bone regeneration process, the anisotropic microstructure of bone tissue (bone quality) recovers much later than bone mass (bone quantity), resulting in severe mechanical dysfunction in the bone. Hence, restoration of bone microstructure in parallel with bone mass is necessary for ideal bone tissue regeneration; for this, development of advanced bifunctional biomaterials, which control both the quality and quantity in regenerated bone, is required. We developed novel oriented bioactive glass/poly(lactic acid) composite scaffolds by introducing an effective methodology for controlling cell alignment and proliferation, which play important roles for achieving bone anisotropy and bone mass, respectively. Our strategy is to manipulate the cell alignment and proliferation by the morphological control of the scaffolds in combination with controlled ion release from bioactive glasses. We quantitatively controlled the morphology of fibermats containing bioactive glasses by electrospinning, which successfully induced cell alignment along the fibermats. Also, the substitution of CaO in Bioglass®(45S5) with MgO and SrO improved osteoblast proliferation, indicating that dissolved Mg 2+ and Sr 2+ ions promoted cell adhesion and proliferation. Our results indicate that the fibermats developed in this work are candidates for the scaffolds to bone tissue regeneration that enable recovery of both bone quality and bone quantity. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1031–1041, 2019.

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“…It has been shown that fiber alignment increases with drum angular speed (RPM) during electrospinning, and tensile properties correlate with alignment because morphology is more uniform and stresses can more easily distribute over all fibers . As a result, once relative humidity was controlled and continuous fibers could be consistently formed, the effects of drum speed were explored via mat tension tests to facilitate choosing a rotation speed for optimal fiber synthesis.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and mechanical properties of para‐aramid nanofibers

Trexler

Hoffman

Smith

et al. 2019

J Polym Sci B Polym Phys

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Scalable, bottom‐up chemical synthesis and electrospinning of novel Cl‐substituted poly(para‐phenylene terephthalamide) (PPTA) nanofibers are herein reported. To achieve Cl‐PPTA nanofibers, the chemical reaction between the monomers was precisely controlled, and dissolution of the polymer into solvent was tailored to enable anisotropic solution formation and sufficient entanglement molecular weight. Electrospinning processing parameters were studied to understand their effects on fiber formation and mat morphology and then optimized to yield consistently high quality fibers. Importantly, the control of relative humidity during the fiber formation process was found to be critical, likely because water promotes hydrogen bond formation between the PPTA chains. The fiber and mat morphologies resulting from different combinations of chemistry and spinning conditions were observed using scanning electron microscopy, and observations were used as inputs to the optimization process. Tensile properties of single Cl‐PPTA nanofibers were characterized for the first time using a nanomanipulator mounted inside a scanning electron microscope (SEM), and fiber moduli measuring up to 70 GPa, and strengths exceeding 1 GPa were achieved. Given the excellent mechanical properties measured for the nanofibers, this chemical synthesis procedure and electrospinning protocol appear to be a promising route for producing a new class of nanofibers with ultrahigh strength and stiffness. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019, 57, 563–573

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Nanofiber Alignment Regulates NIH3T3 Cell Orientation and Cytoskeletal Gene Expression on Electrospun PCL+Gelatin Nanofibers

Cited by 60 publications

References 32 publications

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

Development of bifunctional oriented bioactive glass/poly(lactic acid) composite scaffolds to control osteoblast alignment and proliferation

Synthesis and mechanical properties of para‐aramid nanofibers

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