Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis

Han, Zeyu; Bai, Lang; Zhou, Jing; Qian, Yinhua; Tang, Yunkai; Han, Qibin; Zhang, Xiaoyu; Zhang, Mingzhu; Yang, Xing; Cui, Wenguo; Hao, Yuefeng

doi:10.1016/j.biomaterials.2022.121545

Cited by 23 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, the long-term toxicity profile of local distribution ( Saifi et al, 2018 ) as well as the body wide non-targeting infiltration ( Mohammadpour et al, 2019 ; Wu et al, 2019 ) of nanoparticles are still one of the most important concerns when drug-loaded nano-emusions or nanoparticles were intraarticularly injected. On the contrary, hydrogels spheroids at micro-scale were proven to be more available for the direct release of cells, growth factors and drugs ( Annamalai et al, 2019 ; Volpatti et al, 2021 ), and could provide as a dense barrier for inflammatory erosion and chondrocyte apoptosis ( Wei et al, 2021 ; Han et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury

Sun

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Curcumin has been widely used for the treatment of age-associated diseases, and showed chondroprotective potential for post-traumatic osteoarthritis (OA). However, due to the irregular-shaped and large-sized defects on joint cartilage in degenerated OA, the in vivo delivery and therapeutic effect of curcumin for effective repair remain challenging. In this study, we first present a PEG-GelMA [Poly(Ethylene Glycol) Dimethacrylate-Gelatin Methacrylate, PGMs] hydrogel microgel-based curcumin delivery system for both improved anti-inflammatory and pro-regenerative effects in treatment for cartilage defects. The curcumin-loaded PGMs were produced by a microfluidic system based on light-induced gelation of gelatin methacrylate (GelMA). This PGMs embedding curcumin at a relative low dosage were demonstrated to promote the proliferation and chondrogenic differentiation of mesenchymal stem cells in vitro. More importantly, the PGMs were shown to attenuate the inflammatory response of chondrocytes under IL-1β stimulation. Lastly, the in vivo application of the injectable PGMs significantly promoted the repair of large-sized cartilage injury. These results confirmed that curcumin-loaded PGMs can not only enhance the chondroprotective efficacy under inflammatory conditions but also induce efficient cartilage regeneration. This study provides an advanced strategy with anti-inflammatory and pro-regenerative dual-role therapeutic for treatment of extensive cartilage injuries.

show abstract

Section: Discussionmentioning

confidence: 99%

Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury

Sun

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The medial meniscus was surgically destabilized to prepare the OA model established in the previously study. [ 24 ] To anesthetize the animals, a total of 2% sodium pentobarbital (2.5 mL kg −1 ) was intraperitoneally delivered. The right knee joint was completely exposed after the skin was sliced from the distal patella to the proximal tibial platform.…”

Section: Methodsmentioning

confidence: 99%

Stem Cells Expansion Vector via Bioadhesive Porous Microspheres for Accelerating Articular Cartilage Regeneration

Bai,

Han,

Han

et al. 2023

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Stem cell tissue engineering is a potential treatment for osteoarthritis. However, the number of stem cells that can be delivered, loss of stem cells during injection, and migration ability of stem cells limit applications of traditional stem cell tissue engineering. Herein, kartogenin (KGN)‐loaded poly(lactic‐co‐glycolic acid) (PLGA) porous microspheres was first engineered via emulsification, and then anchored with chitosan through the amidation reaction to develop a new porous microsphere (PLGA‐CS@KGN) as a stem cell expansion vector. Following three‐dimensional co‐culture of the PLGA‐CS@KGN carrier with mesenchymal stem cells (MSCs), the delivery system was injected into the capsule cavity in situ. In vivo and in vitro experiments showed that PLGA‐CS microspheres had a high cell‐carrying capacity up to 1 × 104 mm−3 and provided effective protection of MSCs to promote their controlled release in the osteoarthritis microenvironment. Simultaneously, KGN loaded inside the microspheres effectively cooperated with PLGA‐CS to induce MSCs to differentiate into chondrocytes. Overall, these findings indicated that PLGA‐CS@KGN microspheres held high cell‐loading ability, adapted to the migration and expansion of cells, and promoted MSCs to express markers associated with cartilage repair. Thus, PLGA‐CS@KGN can be used as a potential stem cell carrier for enhancing stem cell therapy in osteoarthritis treatment.This article is protected by copyright. All rights reserved

show abstract

“…Similarly, nano fat (NF) is widely used for treating various diseases due to its rich bioactive components, and its inability to be positioned during implantation limits its biofunctional application. Han et al (2022) immobilized nano fat in microfluidically generated aldehyde-modified poly(lactic-co-glycolic acid) (PLGA) through Schiff base condensation and covalent bonding in a three-dimensional porous network (PMs@NF) porous microspheres, which improved the cartilage targeting efficiency of the nano fats, not only inhibited the progression of OA but also led to significant lubricating properties, reducing the CoF by approximately 80%, with the lowest CoF when the PMs@NF complex ratio was 1:3 (1 N: 0.1393; 2 N: 0.1390; 3 N: 0.1365; 4 N: 0.1837). This material has shown great potential in the treatment of OA.…”

Section: Bio-inspired Cartilage Lubrication Applicationsmentioning

confidence: 99%

Research progress of cartilage lubrication and biomimetic cartilage lubrication materials

Liu

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Human joints move thousands of times a day. The articular cartilage plays a vital role in joints’ protection. If there is dysfunction in cartilage lubrication, cartilage cannot maintain its normal function. Eventually, the dysfunction may bring about osteoarthritis (OA). Extensive researches have shown that fluid film lubrication, boundary lubrication, and hydration lubrication are three discovered lubrication models at cartilage surface, and analyzing and simulating the mechanism of cartilage lubrication are fundamental to the treatment of OA. This essay concludes recent researches on the progress of cartilage lubrication and biomimetic cartilage, revealing the pathophysiology of cartilage lubrication and updating bio-inspired cartilage lubrication applications.

show abstract

Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis

Cited by 23 publications

References 46 publications

Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury

Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury

Stem Cells Expansion Vector via Bioadhesive Porous Microspheres for Accelerating Articular Cartilage Regeneration

Research progress of cartilage lubrication and biomimetic cartilage lubrication materials

Contact Info

Product

Resources

About