Nanoemulsion-templated multilayer nanocapsules for cyanine-type photosensitizer delivery to human breast carcinoma cells

Bazylińska, Urszula; Pietkiewicz, Jadwiga; Saczko, Jolanta; Nattich-Rak, Małgorzata; Rossowska, Joanna; Garbiec, Arnold; Wilk, Kazimiera A.

doi:10.1016/j.ejps.2012.06.019

Cited by 55 publications

(68 citation statements)

References 42 publications

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“…Furthermore, IR-780 encapsulated by nanoemulsions covered by four PE bilayers (nanocapsules 2) photobleaches slightly lower than when enclosed in nanocapsules with thinner coat (nanocapsules 1) (see Table I). The observed phenomenon confirmed the earlier findings, 33,39 indicating that both the nanoparticles' oil core and PE shell thickness may significantly enhance the photostability of the hydrophobic photosensitizer. Other important in vitro features of the encapsulated IR-780 such as reactive oxygen species (ROS) and release characteristics are supplemented in the supplementary material (Figs.…”

Section: -8supporting

confidence: 80%

“…This may be the total effect of both nanocarriers structure and the surface charge of the nanocapsules as similarly observed in our previous FACS study for MCF-7/WT cells treated by IR-786-loaded nanocapsules with different (PSS/PDADMAC) n shell. 33 On the other hand, the cellular uptake of free cyanine IR-780 in A549 cells was almost 2 times higher in relation to the nanocapsules 1 and 2. While in the case of MRC-5 cells we observed an inverse trend, cellular uptake of free cyanine IR-780 was almost 2 times lower.…”

Section: Cellular Uptakementioning

confidence: 99%

“…The imaging of the obtained nanocapsules was investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) according to detailed procedure described our earlier studies. 33 In the case of SEM observation, the shape and morphology of the dry nanocapsules were investigated by field emission SEM (JEOL JSM-7500 F), at an operation voltage of 15 keV. The samples were prepared by placing a drop of nanocapsules suspension on the copper cylinders and drying overnight.…”

Section: Characterization Of Nanocapsule Size Surface Charge and Momentioning

confidence: 99%

“…It also allows improving drug's efficacy, its stability, and better intracellular penetration, owing to their enhanced permeation, specific cell targeting, and long circulation in blood stream. [31][32][33][34][35] We have selected a NIR heptamethine indocyanine dye (i.e., IR-780 iodide) as a model hydrophobic photosensitizer basing mainly upon its excellent optical properties and good photosensitizing attributes. IR-780 indocyanine due to its characteristic excitation (k exc.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

et al. 2016

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The application of nanotechnology is important to improve research and development of alternative anticancer therapies. In order to accelerate research related to cancer diagnosis and to improve the effectiveness of cancer treatment, various nanomaterials are being tested. The main objective of this work was basic research focused on examination of the mechanism and effectiveness of the introduction of nanoencapsulated photosensitizers to human carcinoma (A549) and normal cells . Newly encapsulated hydrophobic indocyanine-type photosensitizer (i.e., IR-780) was subjected to in vitro studies to determine its release characteristics on a molecular level. The photosensitizers were delivered to carcinoma and normal cells cultured under model conditions using multiwell plates and with the use of the specially designed hybrid (poly(dimethylsiloxane) (PDMS)/ glass) microfluidic system. The specific geometry of our microsystem allows for the examination of intercellular interactions between cells cultured in the microchambers connected with microchannels of precisely defined length. Our microsystem allows investigating various therapeutic procedures (e.g., photodynamic therapy) on monoculture, coculture, and mixed culture, simultaneously, which is very difficult to perform using standard multiwell plates. In addition, we tested the cellular internalization of nanoparticles (differing in size, surface properties) in carcinoma and normal lung cells. We proved that cellular uptake of nanocapsules loaded with cyanine IR-780 in carcinoma cells was more significant than in normal cells. We demonstrated non cytotoxic effect of newly synthesized nanocapsules built with polyelectrolytes (PEs) of opposite surface charges: polyanion-polysodium-4-styrenesulphonate and polycation-poly(diallyldimethyl-ammonium) chloride loaded with cyanine IR-780 on human lung carcinoma and normal cell lines. However, the differences observed in the photocytotoxic effect between two types of tested nanocapsules can result from the type of last PE layer and their different surface charge. V C 2016 AIP Publishing LLC. [http://dx

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Section: -8supporting

confidence: 80%

Section: Cellular Uptakementioning

confidence: 99%

Section: Characterization Of Nanocapsule Size Surface Charge and Momentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

et al. 2016

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“…Terapia fotodinâmica (PDT) é outro exemplo de modalidade terapêutica baseada em luz, tendo por característica ser minimamente invasiva e sendo considerada uma alternativa promissora para tratamentos clássicos contra câncer como a remoção cirúrgica, radioterapia e quimioterapia (BAZYLIŃSKA et al, 2012). A PDT baseia-se na internalização de compostos sensíveis à luz, chamados de fotossensibilizadores, pelas células cancerosas alvo, sobre as quais vão exercer uma fototoxicidade seletiva após a iluminação local com luz visível ou na região do infra-vermelho próximo.…”

Section: Terapia Fotodinâmicaunclassified

Ação sinérgica entre terapia fotodinâmica e terapia hipertérmica utilizando nanobarras de ouro

Freitas¹

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A todos aqueles que travam uma batalha diária contra o câncer, passando por sofrimentos inimagináveis, dedico este trabalho, na esperança de ter contribuído um pouco mais na erradicação dessa condição. AGRADECIMENTOSA Deus, pela presença, por todo o amparo e proteção durante toda a minha vida.Aos meus pais, Agostinho e Fátima, pelos exemplos e ensinamentos morais que me garantiram uma conduta idôneae pelo sustento material que garantiu meu percurso.Aos meus irmãos, Leandro, Luciana e Lisiane, os quais me apoiaram desde o início e contribuíram significativamente para que realizasse minhas aspirações.À professora Dra. Ana Maria de Guzzi Plepis, cuja orientação foi fundamental para meu crescimento profissional e intelectual.A Vitor Basso Schul que me apresentou a linha de pesquisa, o laboratório e me incentivou desde a graduação. À Dra. Virgínia C. A. Martins, por todo o auxílio imprescindível e não menos abnegado que dispensou a mim, contribuindo de forma fundamental para a conclusão de meu trabalho.Ao Dr. Nelson Junior, por todo o auxílio com a parte histológica e de biotério, além da amizade em todos esses anos. À professora Dra. Janice Rodrigues Perussi, que gentilmente cedeu alguns dos materiais utilizados neste trabalho.Ao professor Dr. Anderson Ribeiro, da Universidade Federal do ABC, que gentilmente sintetizou e cedeu as ftalocianinas utilizadas neste trabalho. À professora Dra. Cristina Kurachi, juntamente com Dr. Clóvis Grecco, Dra. Lilian Tan Moriyama e Dra. Natalia Inada, os quais me auxiliaram em toda a parte in vivo deste trabalho e me acolhem até hoje de forma calorosa em seu laboratório.A todos os integrantes do Grupo Yanagi Taiko, cujos momentos passados juntos me proporcionaram o alívio dos problemas do dia-a-dia e me fizeram sentir bem acolhido na cidade de São Carlos. Um agradecimento especial a Sônia Mitsue Kaibara, e Guilherme Kawamata, Kamila Kotsubo e Érika Takahashipela amizade sincera.A todos os meus colegas república, de doutorado e de laboratório, com os quais me diverti durante todos os momentos juntos, e aprendi lições preciosas para a vida. Estudos com tratamento hipertérmico de tumores utilizando nanopartículas metálicas têm sido realizados durante as últimas décadas e mostram resultados bons quanto à remissão de tumores, por vezes chegando à cura completa. O mesmo acontece em relação aos tratamentos baseados em ação fotodinâmica de fotossensibilizadores. Tratamentos aliando a terapia hipertérmica com nanopartículas de ouro e a terapia fotodinâmica com diversos fotossensibilizadores tem efeito sinérgico e apresenta excelente potencial terapêutico, em que pese serem necessários mais estudos para que uma nova terapia conjunta possa ser implementada. A proposta deste trabalho foi investigar esse efeito sinérgico utilizando nanobastões de ouro complexados com fotossensibilizadores. Após a síntese dos nanobastões pelo método de seeding, a eficácia do tratamento fotodinâmico e da terapia hipertérmica, separadamente, foi investigada. A metodologia do recobrimento dos nanobastões por fotosse...

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Biocompatible and Biogenic Microcapsules

Zhao

Fei

2015

Layer‐by‐Layer Films for Biomedical Applications

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Nanoemulsion-templated multilayer nanocapsules for cyanine-type photosensitizer delivery to human breast carcinoma cells

Cited by 55 publications

References 42 publications

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Ação sinérgica entre terapia fotodinâmica e terapia hipertérmica utilizando nanobarras de ouro

Biocompatible and Biogenic Microcapsules

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