Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes

Abstract

A transparent oil-in-water nanoemulsion system consisting of eucalyptus oil, Tween-20 as organic phase and water as an aqueous phase was developed using a low energy emulsification method. Physicochemical properties such as droplet size, optical transparency, and long-term stability was studied. The stable eucalyptus oil nanoemulsion (1:2) having mean droplet size in the range of 50-100 nm with a polydispersity index <0.2. The optimized nanoemulsion formulation exhibited significantly higher antibacterial activity by the well diffusion method against Listeria monocytogenes. Further, alteration in the membrane integrity was assessed, and it is found higher for nanoemulsion treated cells than control cells. Atomic force microscopic observations showed distorted morphology of treated bacterial cells. These results propose the possible use of eucalyptus oil nanoemulsion in the food industries. H o w t o c i t e t h i s a r t i c l e : S u g u m a r S , M u k h e r j e e A , Chandrasekaran N. Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes. Asian J Pharm 2015;9:23-8.

Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay

“…Other than that, in the aspect of manipulated surfactant concentration used, all Eucalyptus micellar formulations are reported to become stable as increased of surfactant concentrations. This finding is significant with the other previous literatures, whereas the mean micelles particle diameter decreased with increasing surfactant concentration and formed stable nanoemulsion system through spontaneous emulsification technique 45,46 . Saberi et al .…”

“…Similar result and explanation were stated by Sugumar et al . 45 which developed micellar formulation of antimicrobial Eucalyptus essential oil in nanoemulsion system via spontaneous emulsification.…”

Nanotherapeutics as promising approaches to combat fungal infections

“…They are generally composed of oil, surfactant, cosurfactant, and water. Nanoemulsion have a droplet size which is generally ranging from 10 to 200 nm (Chandrasekaran, 2015), they are homogenous, thermodynamically stable with a transparent or translucent appearance (Figure 3). Additionally, prolonged drug release can be attained (Kogan & Garti, 2006).…”

“…Additionally, prolonged drug release can be attained (Kogan & Garti, 2006). Nanoemulsion have a droplet size which is generally ranging from 10 to 200 nm (Chandrasekaran, 2015), they are homogenous, thermodynamically stable with a transparent or translucent appearance (Figure 3).…”

Formulation and evaluation of phenylephrine and amikacin nanoemulsion

“…Therefore lipid digestion is considered as an important phase to ensure the absorption of carotenoids. However, the bioavailability of bioactive components will be increased due to encapsulation in nanoemulsion, which leads to a reduction in the droplet size (Suyal et al, 2018;Chandrasekaran et al, 2015).…”